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![Immunocytochemistry/Immunofluorescence: HDAC7 Antibody [NBP2-16795] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16795-img0004.jpg "Immunocytochemistry/Immunofluorescence: HDAC7 Antibody [NBP2-16795] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: HDAC7 Antibody [NBP2-16795] - Human colon carcinoma, using HDAC7 antibody at 1:250 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunohistochemistry-Paraffin-NBP2-16795-img0002.jpg)
![Immunoprecipitation: HDAC7 Antibody [NBP2-16795] - 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 ug of HDAC7 antibody 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunoprecipitation-NBP2-16795-img0003.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC7 antibody [C2C3], C-term (NBP2-16795) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16795_rabbit-polyclonal-hdac7-antibody-western-blot-261120251842313.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NB100-56310-img0003.jpg "Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.](http://images.novusbio.com/fullsize/DMC1-Antibody-1D12-4-Western-Blot-NB100-181-img0003.jpg "Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.")
![Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27370-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: 14-3-3 Antibody [NBP2-27370] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27370-img0002.jpg)
![Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-30888-img0008.jpg "Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30888-img0007.jpg)
![Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - A549 xenograft, using SET antibody, isoform 2 specific at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SET-Antibody-Immunohistochemistry-Paraffin-NBP1-30888-img0003.jpg)
![Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Western-Blot-NBP2-44731-img0002.jpg "Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)")
![Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) [NBP2-44731] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Immunohistochemistry-Paraffin-NBP2-44731-img0001.jpg)
![Flow Cytometry: PCNA Antibody (PCNA/694) [NBP2-44731] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Flow-Cytometry-NBP2-44731-img0003.jpg)
![Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SA1-Antibody-1515-Immunoprecipitation-NBP2-43751-img0004.jpg "Immunoprecipitation: SA1 Antibody (1515) [NBP2-43751] - Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 ug of SA1 antibody [1515]. Western blot analysis was performed using SA1 antibody [1515] EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-91020242011502.jpg)
![Western Blot: SA1 Antibody (1515) [NBP2-43751] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-91020242014628.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (NBP2-43751) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-western-blot-2611202518222011.jpg)
![SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SA1 protein stained by SA1 antibody [GT1515] (NBP2-43751) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43751_mouse-monoclonal-sa1-antibody-1515-immunocytochemistry-immunofluorescence-2611202518272316.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.](http://images.novusbio.com/fullsize/NUP160-Antibody-Immunohistochemistry-Paraffin-NBP2-23718-img0001.jpg "Immunohistochemistry-Paraffin: NUP160 Antibody [NBP2-23718] - Analysis of NUP160 in formalin-fixed, paraffin-embedded human brain tissue using preimmune sera (top) and this antibody (bottom) at 1:100.")
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![<b>Independent Antibodies Validation. </b>Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)
![Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0014.jpg "Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.")
![Western Blot: CYLD Antibody [NB100-78600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody (NB100-78600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-78600_rabbit-polyclonal-cyld-antibody-910202420134080.jpg)
