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![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NB100-56310-img0003.jpg "Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [7612].")
![<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 30 ug 293T B. 30 ug A431 C. 30 ug HeLa D. 30 ug HepG2 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:500.](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0001.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0002.jpg)
![Western Blot: Fumarase Antibody (7612) [NBP2-43578] - Analysis of A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [7612] dilution: 1:1000](http://images.novusbio.com/fullsize/Fumarase-Antibody-7612-Western-Blot-NBP2-43578-img0003.jpg)
![Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] (NBP2-43578) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43578_mouse-monoclonal-fumarase-antibody-7612-immunocytochemistry-immunofluorescence-261120251821337.jpg)
![Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.](http://images.novusbio.com/fullsize/SET-Antibody-Western-Blot-NBP1-30888-img0008.jpg "Western Blot: SET Antibody [NBP1-30888] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SET Antibody [NBP1-30888] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SET protein stained by SET antibody, isoform 2-specific diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/SET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30888-img0007.jpg)
![Immunohistochemistry-Paraffin: SET Antibody [NBP1-30888] - A549 xenograft, using SET antibody, isoform 2 specific at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SET-Antibody-Immunohistochemistry-Paraffin-NBP1-30888-img0003.jpg)
![Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Western-Blot-NBP2-44731-img0002.jpg "Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)")
![Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) [NBP2-44731] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Immunohistochemistry-Paraffin-NBP2-44731-img0001.jpg)
![Flow Cytometry: PCNA Antibody (PCNA/694) [NBP2-44731] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Flow-Cytometry-NBP2-44731-img0003.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![<b>Independent Antibodies Validation. </b>Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)
![Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0014.jpg "Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.")
![Western Blot: CYLD Antibody [NB100-78600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody (NB100-78600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-78600_rabbit-polyclonal-cyld-antibody-910202420134080.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0002.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).")
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0001.jpg)
![Western Blot: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRD7-Antibody-Western-Blot-NBP1-28727-img0008.jpg "Western Blot: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (NBP1-28727) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunohistochemistry-Paraffin-NBP1-28727-img0007.jpg)
![Immunoprecipitation: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 (lot NBP1-28727-2) used for IP at 6 ug per reaction. BRD7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-28727-1). For blotting immunoprecipitated BRD7, NBP1-28727 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BRD7-Antibody-Immunoprecipitation-NBP1-28727-img0009.jpg)
![Western Blot: BNC1 Antibody [NBP2-24721] - Analysis of BNC1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using NBP2-24721 at 7 ug/ml and 4 ug/ml, respectively.](http://images.novusbio.com/fullsize/BNC1-Antibody-Western-Blot-NBP2-24721-img0001.jpg "Western Blot: BNC1 Antibody [NBP2-24721] - Analysis of BNC1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using NBP2-24721 at 7 ug/ml and 4 ug/ml, respectively.")
![Western Blot: Gastrokine 1 Antibody [NBP2-24766] - Analysis of GKN1 in human stomach tissue lysate using this antibody. 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Gastrokine-1-Antibody-Western-Blot-NBP2-24766-img0001.jpg "Western Blot: Gastrokine 1 Antibody [NBP2-24766] - Analysis of GKN1 in human stomach tissue lysate using this antibody. 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")