| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04952-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97931SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87249-25ul | |||
![Western Blot: BRSK1 Antibody [NBP3-04952] - Western blot analysis of extracts of Raji cells, using BRSK1 Rabbit pAb (NBP3-04952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/BRSK1-Antibody-Western-Blot-NBP3-04952-img0004.jpg "Western Blot: BRSK1 Antibody [NBP3-04952] - Western blot analysis of extracts of Raji cells, using BRSK1 Rabbit pAb (NBP3-04952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: BRSK1 Antibody [NBP3-04952] - Immunohistochemistry of paraffin-embedded mouse brain using BRSK1 antibody (NBP3-04952) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/BRSK1-Antibody-Immunohistochemistry-Paraffin-NBP3-04952-img0003.jpg)
![Immunohistochemistry-Paraffin: BRSK1 Antibody [NBP3-04952] - Immunohistochemistry of paraffin-embedded rat brain using BRSK1 antibody (NBP3-04952) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/BRSK1-Antibody-Immunohistochemistry-Paraffin-NBP3-04952-img0001.jpg)
![Immunohistochemistry-Paraffin: BRSK1 Antibody [NBP3-04952] - Immunohistochemistry of paraffin-embedded human stomach using BRSK1 antibody (NBP3-04952) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/BRSK1-Antibody-Immunohistochemistry-Paraffin-NBP3-04952-img0002.jpg)
![Western Blot: BRSK1 Antibody - Azide and BSA Free [BRSK1] - Western blot analysis of lysates from Raji cells using BRSK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:10s.](http://images.novusbio.com/fullsize/nbp3-04952_rabbit-polyclonal-brsk1-antibody-5220251632144.jpg)
![Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-27820241631628.jpg "Western Blot: CDC25C Antibody (JE59-53) [NBP3-32173] - Western blot analysis of CDC25C on different lysates with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Caco-2 cell lysate Lane 3: HUVEC cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 58 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32173) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32173) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29820241420958.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC25C Antibody (JE59-53) [NBP3-32173] - Immunocytochemistry analysis of HeLa cells labeling CDC25C with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC25C antibody (NBP3-32173) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-2920241023428.jpg)
![Flow Cytometry: CDC25C Antibody (JE59-53) [NBP3-32173] - Flow cytometric analysis of HeLa cells labeling CDC25C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32173, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32173_rabbit-cdc25c-mab-je59-53-29202416453230.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97936 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97936_rabbit-polyclonal-rfc4-antibody-23520251664787.png)
![Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PIF1-Antibody-Immunohistochemistry-NBP1-97931-img0001.jpg "Immunohistochemistry: PIF1 Antibody [NBP1-97931] - IHC analysis of PIF1 in mouse brain using DAB with hematoxylin counterstain.")
![Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0002.jpg)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0009.jpg "Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CDC25A-Antibody-Western-Blot-NB100-213-img0008.jpg)
![Western Blot: Rabbit Polyclonal CDC25A Antibody [NB100-213] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. CDC25A Antibody (NB100-213) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-213_rabbit-polyclonal-cdc25a-antibody-western-blot-3112024151137..png)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.](http://images.novusbio.com/fullsize/Chk2-Antibody-8F12-Immunocytochemistry-Immunofluorescence-NB100-500-img0003.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.")
![Western Blot: Chk2 Antibody (8F12) [NB100-500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (NB100-500) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-500_mouse-monoclonal-chk2-antibody-8f12-91020242014681.jpg)
![Western Blot: PBK Antibody (3A11) [H00055872-M11] - Analysis of PBK expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/PBK-Antibody-3A11-Western-Blot-H00055872-M11-img0008.jpg "Western Blot: PBK Antibody (3A11) [H00055872-M11] - Analysis of PBK expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: PBK Antibody (3A11) [H00055872-M11] - Analysis of monoclonal antibody to PBK on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PBK-Antibody-3A11-Immunocytochemistry-Immunofluorescence-H00055872-M11-img0006.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody (3A11) [H00055872-M11] - Analysis of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/PBK-Antibody-3A11-Immunohistochemistry-Paraffin-H00055872-M11-img0007.jpg)
![Western Blot: PBK Antibody (3A11) [H00055872-M11] - Analysis of PBK expression in human skeletal muscle.](http://images.novusbio.com/fullsize/PBK-Antibody-3A11-Western-Blot-H00055872-M11-img0009.jpg)
![ELISA: PBK Antibody (3A11) [H00055872-M11] - Detection limit for recombinant GST tagged PBK is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PBK-Antibody-3A11-ELISA-H00055872-M11-img0005.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0011.jpg "Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Immunohistochemistry-Paraffin-H00005984-M01-img0007.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0008.jpg)
![Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - RFC4 monoclonal antibody (M01), clone 1C12 Analysis of RFC4 expression in K-562.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Western-Blot-H00005984-M01-img0010.jpg)
![Sandwich ELISA: RFC4 Antibody (1C12) [H00005984-M01] - Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RFC4-Antibody-1C12-Sandwich-ELISA-H00005984-M01-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87249-img0007.jpg "Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP1-87249] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0011.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0008.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0009.jpg)
![Immunohistochemistry-Paraffin: PPM1D Antibody [NBP1-87249] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in white matter.](http://images.novusbio.com/fullsize/PPM1D-Antibody-Immunohistochemistry-Paraffin-NBP1-87249-img0010.jpg)