| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99017-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27149-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20107-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20098-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52682-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
![Western Blot: PRC1 Antibody [NBP2-99017] - Anti-PRC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: MDA-MB231 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 68 kDa](http://images.novusbio.com/fullsize/PRC1-Antibody-Western-Blot-NBP2-99017-img0002.jpg "Western Blot: PRC1 Antibody [NBP2-99017] - Anti-PRC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: MDA-MB231 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 68 kDa")
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99017] - Immunochemical staining of human PRC1 in human testis with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99017-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99017] - Immunofluorescence staining of PRC1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99017 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-99017_rabbit-polyclonal-prc1-antibody-235202511444419.png)
![Western Blot: GFI1B Antibody [NBP2-84040] - WB Suggested Anti-GFI1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/GFI1B-Antibody-Western-Blot-NBP2-84040-img0003.jpg "Western Blot: GFI1B Antibody [NBP2-84040] - WB Suggested Anti-GFI1B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate")
![Immunohistochemistry-Paraffin: GFI1B Antibody [NBP2-84040] - Rabbit Anti-GFI1B Antibody. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule and renal corpuscle. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/GFI1B-Antibody-Immunohistochemistry-Paraffin-NBP2-84040-img0002.jpg)
![Western Blot: GFI1B Antibody [NBP2-84040] - Host: Rabbit. Target: GFI1B. Positive control (+): Mouse testis (M-TE). Negative control (-): Rat liver (R-LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/GFI1B-Antibody-Western-Blot-NBP2-84040-img0004.jpg)
![Immunohistochemistry-Paraffin: GFI1B Antibody [NBP2-84040] - Rabbit Anti-GFI1B Antibody. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/GFI1B-Antibody-Immunohistochemistry-Paraffin-NBP2-84040-img0001.jpg)
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-278202415242648.jpg "Western Blot: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Western blot analysis of CD30/TNFRSF8 on different lysates with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: HUT 102 cell lysate Lane 3: U-937 cell lysate (negative) Lane 4: HL-60 cell lysate (negative) Lane 5: Daudi cell lysate (negative) Lane 6: HeLa cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 75-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma tissue with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32143) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-298202414333636.jpg)
![Immunocytochemistry/ Immunofluorescence: CD30/TNFRSF8 Antibody (PSH04-11) [NBP3-32143] - Immunocytochemistry analysis of HDLM-2 (positive) and HeLa (negative) labeling CD30/TNFRSF8 with Rabbit anti-CD30/TNFRSF8/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD30/TNFRSF8 antibody (NBP3-32143) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32143_rabbit-cd30-tnfrsf8-mab-psh04-11-29202410334015.jpg)
![Western Blot: TOB1 Antibody [H00010140-B01P] - Analysis of TOB1 expression in transfected 293T cell line by TOB1 polyclonal antibody. Lane 1: TOB1 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TOB1-Antibody-Western-Blot-H00010140-B01P-img0006.jpg "Western Blot: TOB1 Antibody [H00010140-B01P] - Analysis of TOB1 expression in transfected 293T cell line by TOB1 polyclonal antibody. Lane 1: TOB1 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TOB1 Antibody [H00010140-B01P] - Analysis of purified antibody to TOB1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/TOB1-Antibody-Immunocytochemistry-Immunofluorescence-H00010140-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: TOB1 Antibody [H00010140-B01P] - Analysis of purified antibody to TOB1 on formalin-fixed paraffin-embedded human endometrium. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/TOB1-Antibody-Immunohistochemistry-Paraffin-H00010140-B01P-img0005.jpg)
![SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004000-P01-1.jpg "SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-10420249141932.jpg%3Fv=1666704272 "Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (4E12H12) [NBP3-27149] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915839.jpg%3Fv=1666704272)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915849.jpg%3Fv=1666704272)
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024920235.jpg%3Fv=1666704272)
![Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924254.png "Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023964610.png)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924231.png)
![Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924236.png "Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (NBP3-20098, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924292.png)
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30C-18 (NBP3-20098) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20098, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-274202396465.png)
![Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Immunohistochemistry-NBP2-52682-img0001.jpg "Immunohistochemistry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Immunohistochemical staining of human tonsil tissue using anti-CD30 antibody. Ki-3 Anti-CD30 staining of paraffin embedded human tonsil tissue using the rabbit-chimeric version of Ki-3 (NBP2-52682). Antigen retreival was acheived by microwaving in citrate buffer, followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-52682 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of the cellular membrane of dividing cells may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-3) - Chimeric [NBP2-52682] - Flow-cytometry using the anti-CD30 (Tumor necrosis factor receptor superfamily member 8) antibody Ki-3. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Ki-3 (NBP2-52682, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD30-TNFRSF8-Antibody-Ki-3-Chimeric-Flow-Cytometry-NBP2-52682-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48351-img0003.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) - IHC-Prediluted [NBP2-48351] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (DCS-60.2).")