| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50390-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20098-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26414-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26406-50ul | |||
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0004.jpg "Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in cynomolgus heart with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0001.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in human colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0002.jpg)
![Immunohistochemistry-Paraffin: GCR2 Antibody [NBP2-99443] - Immunochemical staining GCR2 in mouse colon with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/GCR2-Antibody-Immunohistochemistry-Paraffin-NBP2-99443-img0003.jpg)
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-278202415212460.jpg "Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Immunocytochemistry analysis of 293T cells labeling ERK3/MAPK6 with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-2920241192388.jpg)
![Flow Cytometry: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Flow cytometric analysis of 293T cells labeling ERK3/MAPK6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32567, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-392024884681.jpg)
![Western Blot: TOB1 Antibody [H00010140-B01P] - Analysis of TOB1 expression in transfected 293T cell line by TOB1 polyclonal antibody. Lane 1: TOB1 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TOB1-Antibody-Western-Blot-H00010140-B01P-img0006.jpg "Western Blot: TOB1 Antibody [H00010140-B01P] - Analysis of TOB1 expression in transfected 293T cell line by TOB1 polyclonal antibody. Lane 1: TOB1 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TOB1 Antibody [H00010140-B01P] - Analysis of purified antibody to TOB1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/TOB1-Antibody-Immunocytochemistry-Immunofluorescence-H00010140-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: TOB1 Antibody [H00010140-B01P] - Analysis of purified antibody to TOB1 on formalin-fixed paraffin-embedded human endometrium. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/TOB1-Antibody-Immunohistochemistry-Paraffin-H00010140-B01P-img0005.jpg)
![Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924236.png "Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (NBP3-20098, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924292.png)
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30C-18 (NBP3-20098) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20098, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-274202396465.png)
![Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-2722024850463.jpg "Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: c-jun [p Ser63] Antibody at 0.95 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa Observed band size: 48 KDa](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-282202411472127.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Staining of A549 cells with c-jun [p Ser63] Antibody (4A11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-28220241312248.jpg)
![Western Blot: CDC2L5 Antibody [NB100-68268] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody NB100-68268 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CDC2L5 was also immunoprecipitated by rabbit anti-CDC2L5 antibody that recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/CDC2L5-Antibody-Western-Blot-NB100-68268-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CDC2L5 Antibody [NB100-68268] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody NB100-68268 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CDC2L5 was also immunoprecipitated by rabbit anti-CDC2L5 antibody that recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes (A) and 10 seconds (B).")
![Western Blot: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-336 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Western-Blot-NB200-336-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-336 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: NCOA6 Antibody [NB200-336] - Detection of Human ASC2 by Western Blot of Immunoprecipitates](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunoprecipitation-NB200-336-img0006.jpg)
![Western Blot: NFIC Antibody [NBP1-71879] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71878, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NFIC-Antibody-Western-Blot-NBP1-71879-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NFIC Antibody [NBP1-71879] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-NFIC antibody used for IP at 6 mcg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody NBP1-71878, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP1-71879-img0003.jpg)
![Immunohistochemistry: NFIC Antibody [NBP1-71879] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- NFIC Cat. No. Lot1, left image used at a dilution of 1:200 (1ug/ml) and Cat. No. Lot1, right image used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/NFIC-Antibody-Immunohistochemistry-NBP1-71879-img0002.jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-2622024152248.jpg "Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: TOR/mTOR [p Ser2448] Antibody at 0.825ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241140938.jpg)
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Staining of Hela cells with TOR/mTOR [p Ser2448] Antibody (1G6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241312233.jpg)
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)