| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21529-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77375-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34683-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37968-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92813-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
![Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-572023156479.jpg "Western Blot: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Western blot analysis using NBP3-21529 in NIH/3T3 cell lysate treated with Anisomycin.")
![Immunohistochemistry: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunohistochemical analysis of paraffin-embedded human breast, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-5720231558484.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.](http://images.novusbio.com/fullsize/nbp3-21529_rabbit-jnk1-2-3-p-thr-183-mab-sr1788-6720231051264.jpg)
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) antibody.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP).](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0005.jpg)
![Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82097(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82097_rabbit-polyclonal-p38-alpha-p-tyr182-antibody-10320252332107.jpg)
![Western Blot: Apc1 Antibody [NBP1-77375] - analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Apc1-Antibody-Western-Blot-NBP1-77375-img0001.jpg "Western Blot: Apc1 Antibody [NBP1-77375] - analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: Apc1 Antibody [NBP1-77375] - Human brain tissue with APC1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Apc1-Antibody-Immunohistochemistry-Paraffin-NBP1-77375-img0002.jpg)
![Flow Cytometry: Apc1 Antibody [NBP1-77375] - APC1 expression in human lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/Apc1-Antibody-Flow-Cytometry-NBP1-77375-img0003.jpg)
![Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-1238] - 3T3 lysates (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/COP9-signalosome-complex-subunit-2-Antibody-Western-Blot-NB100-1238-img0002.jpg "Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-1238] - 3T3 lysates (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry: COP9 signalosome complex subunit 2 Antibody [NB100-1238] - In paraffin embedded Human Thyroid Gland shows nclear and cytoplasm staining in activated epithelial cells Recommended concentration 3-5/ugml.](http://images.novusbio.com/fullsize/COP9-signalosome-complex-subunit-2-Antibody-Immunohistochemistry-NB100-1238-img0014.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0005.jpg "Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml")
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP2-87183] - Rabbit Anti-CHMP4B antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP2-87183-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0002.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Mouse. Target Name: CHMP4B. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0003.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target: CHMP4B. Positive control (+): MCF7 (N10). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0004.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0006.jpg)
![Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) [NBP2-34296] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with TGF alpha Monoclonal Antibody (MF9).](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-MF9-Immunohistochemistry-Paraffin-NBP2-34296-img0001.jpg "Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) [NBP2-34296] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with TGF alpha Monoclonal Antibody (MF9).")
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411545578.jpg "Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: Cullin 1 Antibody [NBP3-37968] - Western blot analysis of various lysates, using Cullin 1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241243916.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-1012202411572689.jpg)
![Immunohistochemistry: Cullin 1 Antibody [NBP3-37968] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37968_rabbit-cullin-1-pab-101220241252829.jpg)
![Western Blot: 14-3-3 zeta/delta Antibody [NBP2-92813] - Analysis of extracts of various cell lines, using 14-3-3 zeta/delta at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/14-3-3-zeta-delta-Antibody-Western-Blot-NBP2-92813-img0003.jpg "Western Blot: 14-3-3 zeta/delta Antibody [NBP2-92813] - Analysis of extracts of various cell lines, using 14-3-3 zeta/delta at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta/delta Antibody [NBP2-92813] - Rat kidney using YWHAZ Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/14-3-3-zeta-delta-Antibody-Immunohistochemistry-Paraffin-NBP2-92813-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/delta Antibody - Azide and BSA Free [NBP2-92813] - Immunofluorescence analysis of paraffin-embedded Rat brain using 14-3-3 zeta/delta Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92813_rabbit-polyclonal-14-3-3-zeta-delta-antibody-42202514251835.jpg)
![Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cullin-3-Antibody-7W3E3-Western-Blot-NBP3-15821-img0004.jpg "Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded rat brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512435347.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded mouse brain tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512432070.jpg)
![Immunohistochemistry: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry analysis of Cullin 3 in paraffin-embedded human colon carcinoma tissue using Cullin 3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15821_rabbit-cullin-3-mab-7w3e3-72202512443371.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH2. Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena.](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0006.jpg "Western Blot: SIAH2 Antibody [NBP2-85730] - Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH2. Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena.")
![Western Blot: SIAH2 Antibody [NBP2-85730] - WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0001.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: HepG2. Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0002.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: MCF7. Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0003.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Tissue: Human OVCAR-3 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0005.jpg)
![Western Blot: SIAH2 Antibody [NBP2-85730] - Host: Rabbit. Target Name: SIAH2. Sample Type: Hela. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide. Primary Antibody Concentration: 1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/lane. Gel Concentration: 0.12](http://images.novusbio.com/fullsize/SIAH2-Antibody-Western-Blot-NBP2-85730-img0004.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)