| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27072SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80972-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73452 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89338-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87592-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86099-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37507-0.025ml | |||
![Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-NBP2-19859-img0013.jpg "Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.")
![Western Blot: Plasminogen Antibody [NBP2-19859] - Plasminogen antibody detects PLG protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGEPlasminogen antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0001.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0002.jpg)
![Western Blot: Plasminogen Antibody [NBP2-19859] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Western-Blot-NBP2-19859-img0003.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0004.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0005.jpg)
![Immunohistochemistry-Paraffin: Plasminogen Antibody [NBP2-19859] - Paraffin-embedded rat prostate Plasminogen antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Plasminogen-Antibody-Immunohistochemistry-Paraffin-NBP2-19859-img0008.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-27072-img0004.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-27072-img0005.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-27072-img0002.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0007.jpg "Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from EL4, mIMCD-3, NIH 3T3,Renca, and TCMK-1 cells prepared using NETN lysis buffer.Antibody: Rabbit anti-E-Cadherin recombinant monoclonalantibody [BLR088G] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] . Secondary:HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRPconjugated goat anti-rabbit IgG. DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0004.jpg)
![Western Blot: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Whole cell lysate (5 ug) from GaMG, HCT 116, U2OS,OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cellsprepared using NETN lysis buffer. Antibody: Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Western-Blot-NBP3-14687-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunocytochemistry-Immunofluorescence-NBP3-14687-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (BLR088G) [NBP3-14687] - Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-BLR088G-Immunohistochemistry-Paraffin-NBP3-14687-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP3-14687] - Whole cell lysate(1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer.Antibodies: Rabbit anti-E-Cadherin recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody. For blotting immunoprecipitated E-Cadherin, was used at 1:1000.Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP3-14687-img0001.jpg)
![Western Blot: SPARC Antibody [NBP1-80972] - Analysis in human cell lines U2OS and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SPARC Antibody [NBP1-80972] - Analysis in human cell lines U2OS and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SPARC Antibody [NBP1-80972] - Analysis using Anti-SPARC antibody NBP1-80972 (A) shows similar pattern to independent antibody NBP1-80971 (B).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SPARC Antibody [NBP1-80972] - Staining SPARC in MG63 cells using anti-SPARC antibody. Cell were fixed in 4% Paraformaldehyde (20 min, RT); and permeabilized in 0,2% Triton (15 min, RT). Image from verified customer review.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80972-img0006.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80972] - Staining of human placenta shows strong positivity in endothelial cells and fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80972-img0011.jpg)
![Western Blot: SPARC Antibody [NBP1-80972] - Analysis of SPARC MC3T3 (mouse), MG63 (human) cell lysates and conditions media using anti-SPARC antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0005.jpg)
![Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Western-Blot-NBP2-02275-img0003.jpg "Western Blot: PKMYT1 Antibody (5E1) [NBP2-02275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1.")
![Immunocytochemistry/Immunofluorescence: PKMYT1 Antibody (OTI5E1) [NBP2-02275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP2-02275-img0014.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human thyroid tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0012.jpg)
![Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Jurkat cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Flow-Cytometry-NBP2-02275-img0002.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0004.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0005.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0006.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human breast tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0007.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human endometrium tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0008.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human Kidney tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0009.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human liver tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0010.jpg)
![Immunohistochemistry-Paraffin: PKMYT1 Antibody (5E1) [NBP2-02275] - Staining of paraffin-embedded Human prostate tissue using anti-PKMYT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Immunohistochemistry-Paraffin-NBP2-02275-img0011.jpg)
![Flow Cytometry: PKMYT1 Antibody (5E1) [NBP2-02275] - Analysis of Hela cells, using anti-PKMYT1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PKMYT1-Antibody-5E1-Flow-Cytometry-NBP2-02275-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0006.jpg "Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunocytochemistry-Immunofluorescence-NBP2-67540-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Flow-Cytometry-NBP2-67540-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0004.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0008.jpg)
![Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Western-Blot-NBP2-89338-img0002.jpg "Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa")
![Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Immunoprecipitation-NBP2-89338-img0001.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T1 (Ccnt1) gene is required for ER stress-mediated CHOP induction and vascular calcification. Immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, and GAPDH proteins in BSA (Veh) or 500 um C18:0 treated scrambled (Scramb.) sg or Ccnt1 KO VSMCs for 6 h. Image collected and cropped by CiteAb from the following publication (//www.jbc.org/lookup/doi/10.1074/jbc.RA118.004706), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Western-Blot-NBP1-87592-img0007.jpg "Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T1 (Ccnt1) gene is required for ER stress-mediated CHOP induction and vascular calcification. Immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, and GAPDH proteins in BSA (Veh) or 500 um C18:0 treated scrambled (Scramb.) sg or Ccnt1 KO VSMCs for 6 h. Image collected and cropped by CiteAb from the following publication (//www.jbc.org/lookup/doi/10.1074/jbc.RA118.004706), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Cyclin T2 Antibody [NBP1-87592] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87592-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin T2 Antibody [NBP1-87592] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Immunohistochemistry-Paraffin-NBP1-87592-img0005.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T2 (Ccnt2) and cyclin K (Ccnk) deficiency induces CHOP expression and calcification, opposite of cyclin T1 deficiency. Immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, and GAPDH proteins in BSA (Veh) or 500 um C18:0 treated scrambled (Scramb.) sg or Ccnt2 KO VSMCs for 6 h. Image collected and cropped by CiteAb from the following publication (//www.jbc.org/lookup/doi/10.1074/jbc.RA118.004706), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyclin-T2-Antibody-Knockout-Validated-NBP1-87592-img0006.jpg)
![Western Blot: Cyclin T2 Antibody [NBP1-87592] - Cyclin T2 (Ccnt2) & cyclin K (Ccnk) deficiency induces CHOP expression & calcification, opposite of cyclin T1 deficiency.A, immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, & GAPDH proteins in BSA (Veh) or 500 μm C18:0 treated scrambled (Scramb.) sg or Ccnt2 KO VSMCs for 6 h. B, qRT-PCR analysis of CHOP in BSA (Veh) or 250 μm C18:0 treated Scramb. sg or Ccnt2 KO VSMCs (n = 4). C, immunoblot analysis of CDK9, cyclin T1, cyclin T2, cyclin K, ATF4, CHOP, & GAPDH proteins in BSA (Veh) or 500 μm C18:0 treated Scramb. sg or Ccnk heterozygous KO VSMCs for 6 h. D, qRT-PCR analysis of CHOP in BSA (Veh) or 250 μm C18:0 treated Scramb. sg or Ccnk heterozygous KO VSMCs (n = 4). E, mineralization of Ccnt2 KO VSMCs. VSMCs were incubated with 2.0 mm phosphate with or without 250 μm C18:0 for 7 days (n = 6). F, mineralization of Ccnk hetero-KO VSMCs. VSMCs were incubated with 2.0 mm phosphate with or without 250 μm C18:0 for 7 days (n = 6). One-way ANOVA with a Student-Newman post hoc test was used for statistical analysis. *, p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30209133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87592_rabbit-polyclonal-cyclin-t2-antibody-310202416114781.jpg)
![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining in human epididymis and skeletal muscle tissues using anti-CUL4B antibody. Corresponding CUL4B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining in human epididymis and skeletal muscle tissues using anti-CUL4B antibody. Corresponding CUL4B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0014.jpg)
![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0013.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0003.jpg "Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-37507-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of colon cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0007.jpg)
![Flow Cytometry: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of Jurkat cells using CDK2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Flow-Cytometry-NBP2-37507-img0005.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mAb against human CDK2 (AA: 197-295) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of cervical cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of CDK2 in colon cancer tissue using anti-CDK2 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-37507-img0008.jpg)
![ELISA: CDK2 Antibody (1A6) [NBP2-37507] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-ELISA-NBP2-37507-img0001.jpg)
![Western Blot: Fibrinogen Antibody [NBP2-80414] - Analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Western-Blot-NBP2-80414-img0004.jpg "Western Blot: Fibrinogen Antibody [NBP2-80414] - Analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Fibrinogen Antibody [NBP2-80414] - Staining Fibrinogen in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80414-img0003.jpg)
![Flow Cytometry: Fibrinogen Antibody [NBP2-80414] - Analysis of NIH-3T3 cells with Fibrinogen antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Flow-Cytometry-NBP2-80414-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrinogen Antibody [NBP2-80414] - Staining Fibrinogen in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80414-img0002.jpg)
