| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26493-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44406 | |||
| Azide and BSA Free | NBP2-34552-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88961-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34502-0.1mg | |||
![Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0066.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM6 Antibody [NB100-251] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM6-Antibody-Immunohistochemistry-NB100-251-img0068.jpg)
![Western Blot: MCM6 Antibody [NB100-251] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM6-Antibody-Western-Blot-NB100-251-img0002.jpg)
![Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-26220241510613.jpg "Immunohistochemistry: Chk1 Antibody (2F2) [NBP3-26658] - Image of Chk1 Antibody (2F2) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Chk1 Antibody (2F2) [NBP3-26658] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate. All lanes: Chk1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44, 51 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-282202411442640.jpg)
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2F2) [NBP3-26658] - Staining of Hela Cells with Chk1 Antibody (2F2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26658_rabbit-chk1-mab-2f2-28220241323640.jpg)
![Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-262202413221524.jpg "Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).")
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-26220241547561.jpg)
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-2722024845325.jpg)
![Western Blot: ATM Antibody (4E11) [NBP3-26493] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate. All lanes: ATM Antibody at 2.05ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 350 KDa Observed band size: 350 KDa](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-28220241140928.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) [NBP2-32954] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cdc20 Antibody (AR12).](http://images.novusbio.com/fullsize/Cdc20-Antibody-AR12-Immunohistochemistry-Paraffin-NBP2-32954-img0003.jpg "Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) [NBP2-32954] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cdc20 Antibody (AR12).")
![Immunohistochemistry-Paraffin: Cdc20 Antibody (AR12) [NBP2-32954] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Cdc20 Antibody (AR12).](http://images.novusbio.com/fullsize/Cdc20-Antibody-AR12-Immunohistochemistry-Paraffin-NBP2-32954-img0002.jpg)
![Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (50 ug) from HeLa cells treated with 500 uM EPE for 4 hours (+) or mock treated (-) cells Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody used for WB at 1 ug/ml (upper panel); to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 500 uM EPE for 4 hours (+). Antibodies: Phospho SMC3 was immunoprecipitated (IP) using rabbit anti-Phospho SMC3 (S1083) antibody. The IP reactions were either mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody was used for western blot (WB) of IPs at 1 ug/ml (upper panel); to detect total SMC3 affinity purified rabbit anti-SMC3 was used for WB of IPs at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) and 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Immunoprecipitation-NBP2-37694-img0004.jpg)
![SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot of immunoprecipitates. Samples: Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Phospho SMC3 (S1083) was immunoprecipitated using rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 at 6 ug per reaction. Total SMC3 was immunoprecipitated with another rabbit anti-SMC3 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-NBP2-37694-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32153-img0007.jpg "Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/UbcH3-Cdc34-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32153-img0004.jpg)
![UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32153_rabbit-polyclonal-ube2r1-cdc34-antibody-western-blot-152023124356..jpg)
![Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP1-88961-img0005.jpg "Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: PPP2R5C Antibody [NBP1-88961] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Immunohistochemistry-Paraffin-NBP1-88961-img0002.jpg)
![Western Blot: Orc2 Antibody [NBP1-46176] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46176 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/Orc2-Antibody-Western-Blot-NBP1-46176-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Orc2 Antibody [NBP1-46176] - Detection of human ORC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody NBP1-46176 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Orc2 Antibody [NBP1-46176] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/Orc2-Antibody-Immunohistochemistry-NBP1-46176-img0003.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0007.jpg "Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.")
![Immunohistochemistry: E2F-1 [p Ser364] Antibody [NB600-900] - Affinity Purified E2F-1 [p Ser364] antibody was used at a 10 ug/ml to detect nuclear and occasionally cytoplasmic signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F-1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Immunohistochemistry-NB600-900-img0008.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - NB600-900 Western blot - E2F1 (phospho S364) antibody](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0001.jpg)
![Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-[p-Ser317]-Antibody-Western-Blot-NBP2-37703-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Chk1, Phospho (S317) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Chk1-[p-Ser317]-Antibody-Immunohistochemistry-NBP2-37703-img0003.jpg)
![Western Blot: CDC45L Antibody [NBP2-15834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP2-15834-img0004.jpg "Western Blot: CDC45L Antibody [NBP2-15834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDC45L antibody diluted at 1:1000.")
![Western Blot: CDC45L Antibody [NBP2-15834] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CDC45L-Antibody-Western-Blot-NBP2-15834-img0001.jpg)
![CDC45L antibody [N3C3] detects CDC45L protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDC45L protein stained by CDC45L antibody [N3C3] (NBP2-15834) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15834_rabbit-polyclonal-cdc45l-antibody-immunocytochemistry-immunofluorescence-261120251826136.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TS106) [NBP2-34445] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TS106).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-TS106-Immunohistochemistry-Paraffin-NBP2-34445-img0002.jpg "Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TS106) [NBP2-34445] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TS106).")