| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12892-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97603-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99648-50ul | |||
![Western Blot: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Western blot analysis of extracts of various cell lines, using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-5G5L9-Western-Blot-NBP3-16588-img0002.jpg "Western Blot: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Western blot analysis of extracts of various cell lines, using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (5G5L9) [NBP3-16588] - Immunofluorescence analysis of U-2 OS cells using UbcH10/UBE2C Rabbit mAb (NBP3-16588) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-5G5L9-Immunocytochemistry-Immunofluorescence-NBP3-16588-img0001.jpg)
![Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CDCA5-Antibody-1Y1C9-Western-Blot-NBP3-16318-img0002.jpg "Western Blot: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Western blot analysis of extracts of HepG2 cells, using CDCA5 Rabbit mAb (NBP3-16318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: CDCA5 Antibody (1Y1C9) [NBP3-16318] - Immunohistochemistry of paraffin-embedded human thyroid cancer using CDCA5 Rabbit mAb (NBP3-16318) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDCA5-Antibody-1Y1C9-Immunohistochemistry-Paraffin-NBP3-16318-img0001.jpg)
![Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/CENPA-Antibody-5A7-2E11-Western-Blot-NBP2-12892-img0002.jpg "Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11 . Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/CENPA-Antibody-5A7-2E11-Immunocytochemistry-Immunofluorescence-NBP2-12892-img0003.jpg)
![Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP2-97603-img0003.jpg "Western Blot: NCAPH Antibody [NBP2-97603] - Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa")
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP2-97603] - Immunofluorescence staining of NCAPH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97603 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97603_rabbit-polyclonal-ncaph-antibody-23520251433150.png)
![Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRC1-Antibody-Immunohistochemistry-Paraffin-NBP2-99648-img0002.jpg "Immunohistochemistry-Paraffin: PRC1 Antibody [NBP2-99648] - Immunochemical staining of human PRC1 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: PRC1 Antibody [NBP2-99648] - Immunofluorescence staining of PRC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99648 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-99648_rabbit-polyclonal-prc1-antibody-23520251628112.png)
![Western Blot: Apc2 Antibody [NBP2-87013] - WB Suggested Anti-APC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta](http://images.novusbio.com/fullsize/Apc2-Antibody-Western-Blot-NBP2-87013-img0002.jpg "Western Blot: Apc2 Antibody [NBP2-87013] - WB Suggested Anti-APC2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta")
![Immunohistochemistry: Apc2 Antibody [NBP2-87013] - Skin](http://images.novusbio.com/fullsize/Apc2-Antibody-Immunohistochemistry-NBP2-87013-img0001.jpg)
![SMC5 Antibody [NBP3-12159] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/SMC5-Antibody-NBP3-12159-img0001.jpg "SMC5 Antibody [NBP3-12159] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![SMC5 Antibody [NBP3-12159] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/SMC5-Antibody-NBP3-12159-img0002.jpg)
![Western Blot: Cullin 3 Antibody [NBP2-88798] - WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NBP2-88798-img0002.jpg "Western Blot: Cullin 3 Antibody [NBP2-88798] - WB Suggested Anti-CUL3 Antibody Titration: 1 ug/ml. Positive Control: Fetal liver cell lysate")
![Immunohistochemistry: Cullin 3 Antibody [NBP2-88798] - Immunohistochemistry with Testis tissue at an antibody concentration of 10ug/ml using anti-CUL3 antibody](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NBP2-88798-img0001.jpg)
![Western Blot: MCM4 Antibody (JE38-76) [NBP3-32578] - Western blot analysis of MCM4 on different lysates with Rabbit anti-MCM4 antibody (NBP3-32578) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32578) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32578_rabbit-mcm4-mab-je38-76-27820241529551.jpg "Western Blot: MCM4 Antibody (JE38-76) [NBP3-32578] - Western blot analysis of MCM4 on different lysates with Rabbit anti-MCM4 antibody (NBP3-32578) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32578) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM4 Antibody (JE38-76) [NBP3-32578] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MCM4 antibody (NBP3-32578) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32578_rabbit-mcm4-mab-je38-76-298202414161853.jpg)
![Western Blot: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32174) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32174_rabbit-cdc6-p-ser54-mab-je59-52-278202416285220.jpg "Western Blot: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32174) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32174) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32174_rabbit-cdc6-p-ser54-mab-je59-52-29820241420921.jpg)
![Western Blot: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934] - Western blot analysis of SCP3/SYCP3 on SCP3 recombinant protein with Rabbit anti-SCP3/SYCP3 antibody (NBP3-32934) at 1/1,000 dilution. Lysates/proteins at 30 ng/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32934) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32934_rabbit-scp3-sycp3-mab-psh02-24-288202412461684.jpg "Western Blot: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934] - Western blot analysis of SCP3/SYCP3 on SCP3 recombinant protein with Rabbit anti-SCP3/SYCP3 antibody (NBP3-32934) at 1/1,000 dilution. Lysates/proteins at 30 ng/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32934) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SCP3/SYCP3 antibody (NBP3-32934) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32934) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32934_rabbit-scp3-sycp3-mab-psh02-24-29820241430418.jpg)