| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30589-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71373 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-439SS | |||
| Azide and BSA Free | NBP2-80945 | |||
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0009.jpg "Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.")
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92122 (A) shows similar protein distribution across tissues to independent antibody NBP1-92123 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0006.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0007.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0010.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-92122_rabbit-polyclonal-mkks-antibody-29520251625315.jpg)
![Immunocytochemistry/Immunofluorescence: FAM107A Antibody [NBP2-30589] - Staining of human cell line HUVEC TERT2 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30589-img0007.jpg "Immunocytochemistry/Immunofluorescence: FAM107A Antibody [NBP2-30589] - Staining of human cell line HUVEC TERT2 shows localization to nuclear speckles. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining in human fallopian tube and liver tissues . Corresponding FAM107A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0013.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human cerebellum shows moderate to strong positivity.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0008.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human fallopian tube shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0009.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human hippocampus shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0010.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0011.jpg)
![Immunohistochemistry-Paraffin: FAM107A Antibody [NBP2-30589] - Staining of human prostate shows moderate nuclear positivity.](http://images.novusbio.com/fullsize/FAM107A-Antibody-Immunohistochemistry-Paraffin-NBP2-30589-img0012.jpg)
![Western Blot: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of extracts (35ug) from 9 different cell lines by using CUL4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CUL4B-Antibody-1C4-Western-Blot-NBP2-45648-img0005.jpg "Western Blot: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of extracts (35ug) from 9 different cell lines by using CUL4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/CUL4B-Antibody-1C4-Immunohistochemistry-NBP2-45648-img0004.jpg)
![Western Blot: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUL4B.](http://images.novusbio.com/fullsize/CUL4B-Antibody-1C4-Western-Blot-NBP2-45648-img0006.jpg)
![Immunohistochemistry: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/CUL4B-Antibody-1C4-Immunohistochemistry-NBP2-45648-img0001.jpg)
![Immunohistochemistry: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/CUL4B-Antibody-1C4-Immunohistochemistry-NBP2-45648-img0002.jpg)
![Immunohistochemistry: CUL4B Antibody (1C4) [NBP2-45648] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/CUL4B-Antibody-1C4-Immunohistochemistry-NBP2-45648-img0003.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Western Blot: LDOC1 Antibody [NBP1-80323] - Host: Rabbit. Target: LDOC1. Positive control (+): MCF7 (N10). Negative control (-): HeLa (HL). Antibody concentration: 3ug/ml](http://images.novusbio.com/fullsize/LDOC1-Antibody-Western-Blot-NBP1-80323-img0006.jpg "Western Blot: LDOC1 Antibody [NBP1-80323] - Host: Rabbit. Target: LDOC1. Positive control (+): MCF7 (N10). Negative control (-): HeLa (HL). Antibody concentration: 3ug/ml")
![Immunohistochemistry: LDOC1 Antibody [NBP1-80323] - Human Thyroid lysate tissue at an antibody concentration of 5.0 ug/ml using anti-LDOC1 antibody](http://images.novusbio.com/fullsize/LDOC1-Antibody-Immunohistochemistry-NBP1-80323-img0005.jpg)
![Western Blot: LDOC1 Antibody [NBP1-80323] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/LDOC1-Antibody-Western-Blot-NBP1-80323-img0001.jpg)
![Immunohistochemistry: LDOC1 Antibody [NBP1-80323] - Human brain, cortex tissue at an antibody concentration of 5.0 ug/ml using anti-LDOC1 antibody](http://images.novusbio.com/fullsize/LDOC1-Antibody-Immunohistochemistry-NBP1-80323-img0003.jpg)
![Immunohistochemistry: LDOC1 Antibody [NBP1-80323] - Human breast tissue at an antibody concentration of 5.0 ug/ml using anti-LDOC1 antibody](http://images.novusbio.com/fullsize/LDOC1-Antibody-Immunohistochemistry-NBP1-80323-img0004.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/PBK-Antibody-3A7-Western-Blot-H00055872-M07-img0010.jpg "Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: PBK Antibody (3A7) [H00055872-M07] - Analysis of monoclonal antibody to PBK on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PBK-Antibody-3A7-Immunocytochemistry-Immunofluorescence-H00055872-M07-img0006.jpg)
![Immunohistochemistry-Paraffin: PBK Antibody (3A7) [H00055872-M07] - Analysis of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/PBK-Antibody-3A7-Immunohistochemistry-Paraffin-H00055872-M07-img0008.jpg)
![Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in 293 (Cat # L026V1).](http://images.novusbio.com/fullsize/PBK-Antibody-3A7-Western-Blot-H00055872-M07-img0009.jpg)
![Western Blot: PBK Antibody (3A7) [H00055872-M07] - Analysis of PBK expression in Y-79 (Cat # L042V1).](http://images.novusbio.com/fullsize/PBK-Antibody-3A7-Western-Blot-H00055872-M07-img0011.jpg)
![Western Blot: CDK5 Activator 1 Antibody [NBP3-13279] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:1000 and competitor's antibody diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Western-Blot-NBP3-13279-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CDK5 Activator 1 Antibody [NBP3-13279] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:1000 and competitor's antibody diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13279-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunohistochemistry-Paraffin-NBP3-13279-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK5-Activator-1-Antibody-Immunohistochemistry-Paraffin-NBP3-13279-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411531046.jpg "Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from Mouse heart , using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120621.jpg)
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from U-87MG cells, using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535820.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of NIH/3T3 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120660.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of C6 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535868.jpg)
![Western Blot: CEND1 Antibody (1D3Z1) [NBP3-16614] - Western blot analysis of extracts of various cell lines, using CEND1 Rabbit mAb (NBP3-16614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Western-Blot-NBP3-16614-img0003.jpg "Western Blot: CEND1 Antibody (1D3Z1) [NBP3-16614] - Western blot analysis of extracts of various cell lines, using CEND1 Rabbit mAb (NBP3-16614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Immunofluorescence analysis of mouse brain cells using CEND1 Rabbit mAb (NBP3-16614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16614-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Immunofluorescence analysis of rat brain cells using CEND1 Rabbit mAb (NBP3-16614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CEND1-Antibody-1D3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16614-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Confocal imaging of paraffin-embedded Rat brain tissue using CEND1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16614_rabbit-cend1-mab-1d3z1-5220251242528.jpg)
![Immunocytochemistry/ Immunofluorescence: CEND1 Antibody (1D3Z1) [NBP3-16614] - Confocal imaging of paraffin-embedded Mouse brain tissue using CEND1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16614_rabbit-cend1-mab-1d3z1-52202511383719.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.](http://images.novusbio.com/fullsize/Separase-Antibody-XJ11-1B12-Western-Blot-NB100-439-img0002.jpg "Western Blot: Separase Antibody (XJ11-1B12) [NB100-439] - Separase detected in 5C6 cell lysates using NB 100-439. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone.")