| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52480-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52481-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04967-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83436-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83274-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48133 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76518-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26235-50ul | |||
![Western Blot: HDAC9 Antibody (3L5E5) [NBP3-16097] - Western blot analysis of extracts of various cell lines, using HDAC9 Rabbit mAb (NBP3-16097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/HDAC9-Antibody-3L5E5-Western-Blot-NBP3-16097-img0004.jpg "Western Blot: HDAC9 Antibody (3L5E5) [NBP3-16097] - Western blot analysis of extracts of various cell lines, using HDAC9 Rabbit mAb (NBP3-16097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097] - Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC9 Rabbit mAb (NBP3-16097) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HDAC9-Antibody-3L5E5-Immunohistochemistry-Paraffin-NBP3-16097-img0002.jpg)
![Immunohistochemistry-Paraffin: HDAC9 Antibody (3L5E5) [NBP3-16097] - Immunohistochemistry of paraffin-embedded rat testis using HDAC9 Rabbit mAb (NBP3-16097) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HDAC9-Antibody-3L5E5-Immunohistochemistry-Paraffin-NBP3-16097-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC9 Antibody (3L5E5) [NBP3-16097] - Confocal imaging of U-2 OS cells using HDAC9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16097_rabbit-hdac9-mab-3l5e5-52202511365133.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC9 Antibody (3L5E5) [NBP3-16097] - Confocal imaging of K-562 cells using HDAC9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16097_rabbit-hdac9-mab-3l5e5-5220251215478.jpg)
![Western Blot: RBBP4/RbAp48 Antibody [NB100-60399] - Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: NB100-60399 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-Western-Blot-NB100-60399-img0004.jpg "Western Blot: RBBP4/RbAp48 Antibody [NB100-60399] - Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: NB100-60399 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate.")
![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NB100-60399] - Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti- RBBP4/RbAp48 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-Immunohistochemistry-NB100-60399-img0008.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Western-Blot-NBP2-52480-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)")
![Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Analysis of paraffin-embedded endometrial cancer tissues using HDAC4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Immunohistochemistry-NBP2-52480-img0002.jpg)
![Flow Cytometry: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Flow-Cytometry-NBP2-52480-img0001.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-Western-Blot-NBP2-52480-img0004.jpg)
![ELISA: Histone Deacetylase 4/HDAC4 Antibody (5E4A5) [NBP2-52480] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-5E4A5-ELISA-NBP2-52480-img0005.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Western-Blot-NBP2-52481-img0003.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Analysis of HeLa cells using HDAC4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Immunofluorescence-NBP2-52481-img0002.jpg)
![Flow Cytometry: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Flow-Cytometry-NBP2-52481-img0001.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-Western-Blot-NBP2-52481-img0004.jpg)
![ELISA: Histone Deacetylase 4/HDAC4 Antibody (7E2E6) [NBP2-52481] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-7E2E6-ELISA-NBP2-52481-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/HDAC4+5+9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-04967-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC4+5+9 Antibody [NBP1-04967] - Staining of methanol-fixed Hela cells.")
![Western Blot: HDAC4+5+9 Antibody [NBP1-04967] - Western blot analysis of extracts from 293 and Hela cells using NBP1-04967](http://images.novusbio.com/fullsize/nbp1-04967_rabbit-polyclonal-hdac4-5-9-antibody-23420241523251.jpg)
![Immunohistochemistry-Paraffin: HDAC4+5+9 Antibody [NBP1-04967] - Paraffin-embedded human lung carcinoma tissue using NBP1-04967(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nbp1-04967_rabbit-polyclonal-hdac4-5-9-antibody-103202523464514.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-26220241422521.jpg "Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-2722024850460.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate. All lanes: RBBP4/RbAp48 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-28220241135166.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Staining of Hela Cells with RBBP4/RbAp48 Antibody (3E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-282202412475121.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP1-83436] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles, cytosol & the Golgi apparatus.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83436-img0004.jpg "Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP1-83436] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles, cytosol & the Golgi apparatus.")
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human skin shows moderate cytoplasmic positivity and strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0007.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human placenta shows moderate cytoplasmic positivity and strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0005.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human skeletal muscle shows weak cytoplasmic and nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0006.jpg)
![Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP1-83436] - Staining of human cerebellum shows weak cytoplasmic positivity and moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/HDAC5-Antibody-Immunohistochemistry-Paraffin-NBP1-83436-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC9 Antibody [NBP1-83274] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HDAC9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83274-img0004.jpg "Immunocytochemistry/Immunofluorescence: HDAC9 Antibody [NBP1-83274] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (KIP2/880) - Azide and BSA Free [NBP2-47766] - Human Prostate Carcinoma stained with p57 Monoclonal Antibody (KIP2/880).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-KIP2-880-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47766-img0001.jpg "Immunohistochemistry-Paraffin: p57 Kip2 Antibody (KIP2/880) - Azide and BSA Free [NBP2-47766] - Human Prostate Carcinoma stained with p57 Monoclonal Antibody (KIP2/880).")
![Immunohistochemistry-Paraffin: p57 Kip2 Antibody (KIP2/880) - Azide and BSA Free [NBP2-47766] - Human Colon Carcinoma stained with p57 Monoclonal Antibody (KIP2/880).](http://images.novusbio.com/fullsize/p57-Kip2-Antibody-KIP2-880-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47766-img0002.jpg)
![Western Blot: ZBTB5 Antibody [NBP1-76518] - Mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ZBTB5-Antibody-Western-Blot-NBP1-76518-img0001.jpg "Western Blot: ZBTB5 Antibody [NBP1-76518] - Mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: ZBTB5 Antibody - BSA Free [NBP1-76518] - Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76518_rabbit-polyclonal-zbtb5-antibody-2711202420115566.jpg)
![Immunocytochemistry/ Immunofluorescence: ZBTB5 Antibody - BSA Free [NBP1-76518] - Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76518_rabbit-polyclonal-zbtb5-antibody-2711202419333471.jpg)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)
![Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] - Image of HDAC9 Antibody (1F2) diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26235_rabbit-hdac9-mab-1f2-27220248274916.jpg "Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] - Image of HDAC9 Antibody (1F2) diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: HDAC9 Antibody (1F2) [NBP3-26235] - Image of HDAC9 Antibody (1F2) diluted at 1:154 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26235_rabbit-hdac9-mab-1f2-2722024856313.jpg)
![Western Blot: HDAC9 Antibody (1F2) [NBP3-26235] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. All lanes: HDAC9 Antibody at 1.54ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 112, 102, 66, 98, 118, 113, 61, 63, 58 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26235_rabbit-hdac9-mab-1f2-28220241136301.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC9 Antibody (1F2) [NBP3-26235] - Staining of Hela cells with HDAC9 Antibody (1F2) at 1:51, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26235_rabbit-hdac9-mab-1f2-28220241247519.jpg)