| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84322-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48318 | |||
| Azide and BSA Free | NBP3-11498 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72134 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89346-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76549-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71125 | |||
![Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84322] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84322-img0010.jpg "Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84322] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0021.jpg)
![Western Blot: STIM1 Antibody [NBP1-84322] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/STIM1-Antibody-Western-Blot-NBP1-84322-img0007.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0013.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts..](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0014.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84322 (A) shows similar protein distribution across tissues to independent antibody NBP1-84323 (B).](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0020.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54548-img0001.jpg "Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).")
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or exosomes stained with CF568-labeled CD81 monoclonal antibody (1.3.3.22) (orange).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54548-img0007.jpg)
![Immunohistochemistry-Paraffin: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54548-img0002.jpg)
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - Flow cytometry analysis of MCF-7 cells unstained (gray) or stained with CD81 mouse monoclonal antibody (1.3.3.22) and goat anti-mouse CF647 (red).](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54548-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: CD81 Antibody (1.3.3.22) - Azide and BSA Free [NBP2-54548] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP2-53318) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Azide-and-BSA-Free-Knockout-Validated-NBP2-54548-img0005.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0006.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence analysis of HeLa cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) (green). Nuclei counterstained stained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) [NBP2-44611] - Human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunohistochemistry-Paraffin-NBP2-44611-img0001.jpg)
![Flow Cytometry: Nucleolin Antibody (SPM614) [NBP2-44611] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolin Antibody (SPM614).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Flow-Cytometry-NBP2-44611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of HeLa cells stained with CF647R labeled Nucleolin Antibody (SPM614)(Green) Blue: DAPI.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (SPM614) [NBP2-44611] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (SPM614) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-SPM614-Immunocytochemistry-Immunofluorescence-NBP2-44611-img0005.jpg)
![Western Blot: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEPRE1.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Western-Blot-NBP2-45786-img0011.jpg "Western Blot: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEPRE1.")
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0010.jpg)
![Flow Cytometry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by LEPRE1 antibody.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Flow-Cytometry-NBP2-45786-img0001.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0002.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0003.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0004.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0005.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0006.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0007.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0008.jpg)
![Immunohistochemistry: LEPRE1 Antibody (1H3) [NBP2-45786] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/LEPRE1-Antibody-1H3-Immunohistochemistry-NBP2-45786-img0009.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-western-blot-141120251842302.jpg "Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-western-blot-1411202518433322.jpg)
![Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-western-blot-141120251829413.jpg)
![<b>Orthogonal Strategies Validation. </b>HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-western-blot-1411202518273412.jpg)
![GPS2 antibody [HL2276] detects GPS2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse salivary gland.GPS2 stained by GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-immunohistochemistry-paraffin-141120251842416.jpg)
![GPS2 antibody [HL2276] detects GPS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.GPS2 stained by GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-immunohistochemistry-paraffin-1411202518423016.jpg)
![GPS2 antibody [HL2276] detects GPS2 protein at cytoplasm by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPS2 stained by GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48675_rabbit-gps2-mab-hl2276-immunocytochemistry-immunofluorescence-141120251839102.jpg)
![Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-LGALS3BP antibody. Corresponding LGALS3BP RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Western-Blot-NBP1-89346-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-LGALS3BP antibody. Corresponding LGALS3BP RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-89346-img0020.jpg)
![Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-89346-img0017.jpg)
![Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-89346-img0018.jpg)
![Immunohistochemistry-Paraffin: Galectin-3BP/MAC-2BP/LGALS3BP Antibody [NBP1-89346] - Staining of human skeletal muscle shows no cytoplasmic positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-Western-Blot-NBP1-89346-img0019.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0010.jpg "Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.")
![Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 degrees C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0003.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0009.jpg)
![Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunoprecipitation-NB600-915-img0005.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Twenty one more markedly oxidized proteins identified in 6‐week‐old PLP‐tg66/66 mice in the spinal cord. All non‐mitochondrial oxidized proteins tested by Western blot in the spinal cord are normally expressed. Relative protein level expressed as a percentage of control and in reference to gamma ‐tubulin as a loading marker (n = 6/genotype). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29027761/) licensed under a CC-BY license](http://images.novusbio.com/fullsize/nb600-915_goat-polyclonal-aldolase-a-antibody-2552023153959.jpg)
![Western Blot: TM4SF1/L6 Antibody [NBP1-76549] - Human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TM4SF1-L6-Antibody-Western-Blot-NBP1-76549-img0001.jpg "Western Blot: TM4SF1/L6 Antibody [NBP1-76549] - Human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry-Paraffin: TM4SF1/L6 Antibody [NBP1-76549] - Mouse lung tissue with TM4SF1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/TM4SF1-L6-Antibody-Immunohistochemistry-Paraffin-NBP1-76549-img0010.jpg)
![Immunohistochemistry-Paraffin: TM4SF1/L6 Antibody [NBP1-76549] - Human lung tissue with TM4SF1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TM4SF1-L6-Antibody-Immunohistochemistry-Paraffin-NBP1-76549-img0003.jpg)
![Immunohistochemistry: TM4SF1/L6 Antibody - BSA Free [NBP1-76549] - Immunohistochemistry of TM4SF1/L6 in mouse lung tissue with TM4SF1/L6 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76549_rabbit-polyclonal-tm4sf1-l6-antibody-27112024209207.gif)
![Immunocytochemistry/ Immunofluorescence: TM4SF1/L6 Antibody - BSA Free [NBP1-76549] - Immunofluorescence of TM4SF1/L6 in human lung tissue with TM4SF1/L6 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76549_rabbit-polyclonal-tm4sf1-l6-antibody-271120242004125.jpg)
![Immunocytochemistry/ Immunofluorescence: TM4SF1/L6 Antibody - BSA Free [NBP1-76549] - Immunofluorescence of TM4SF1/L6 in mouse lung tissue with TM4SF1/L6 antibody at 20 ug/mL.Red: TM4SF1/L6 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76549_rabbit-polyclonal-tm4sf1-l6-antibody-271120242022919.gif)
![Western Blot: LGALS3BP Antibody (6B7) [NBP2-01160] Analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3BP monoclonal antibody.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Western-Blot-NBP2-01160-img0008.jpg "Western Blot: LGALS3BP Antibody (6B7) [NBP2-01160] Analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3BP monoclonal antibody.")
![Immunohistochemistry-Paraffin: LGALS3BP Antibody (6B7) [NBP2-01160] - Staining of paraffin-embedded Human prostate tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Immunohistochemistry-Paraffin-NBP2-01160-img0007.jpg)
![Flow Cytometry: LGALS3BP Antibody (6B7) [NBP2-01160] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGALS3BP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Flow-Cytometry-NBP2-01160-img0001.jpg)
![Western Blot: LGALS3BP Antibody (6B7) [NBP2-01160] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Western-Blot-NBP2-01160-img0002.jpg)
![Immunohistochemistry-Paraffin: LGALS3BP Antibody (6B7) [NBP2-01160] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Immunohistochemistry-Paraffin-NBP2-01160-img0003.jpg)
![Immunohistochemistry-Paraffin: LGALS3BP Antibody (6B7) [NBP2-01160] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Immunohistochemistry-Paraffin-NBP2-01160-img0004.jpg)
![Immunohistochemistry-Paraffin: LGALS3BP Antibody (6B7) [NBP2-01160] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Immunohistochemistry-Paraffin-NBP2-01160-img0005.jpg)
![Immunohistochemistry-Paraffin: LGALS3BP Antibody (6B7) [NBP2-01160] - Staining of paraffin-embedded Human pancreas tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LGALS3BP-Antibody-6B7-Immunohistochemistry-Paraffin-NBP2-01160-img0006.jpg)
![Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Western-Blot-NBP2-71125-img0002.jpg "Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.")
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Human pancreas tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0007.jpg)
![Flow Cytometry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LGALS3BP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71125-img0003.jpg)
![Western Blot: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LGALS3BP monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Western-Blot-NBP2-71125-img0001.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0004.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0005.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0006.jpg)
![Immunohistochemistry: Galectin-3BP/MAC-2BP/LGALS3BP Antibody (OTI6B7) - Azide and BSA Free [NBP2-71125] - Staining of paraffin-embedded Human prostate tissue using anti-LGALS3BP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Galectin-3BP-MAC-2BP-LGALS3BP-Antibody-OTI6B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71125-img0008.jpg)