| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27403 | |||
| BSA Free | NB100-56546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-1487SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1768SS | |||
| Azide and BSA Free | NBP2-80668 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80610-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27072SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52501-0.025mg | |||
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-27403-img0006.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-27403-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Flow-Cytometry-NBP2-27403-img0005.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Azide-Free-Western-Blot-NBP2-27403-img0001.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0003.jpg "Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunocytochemistry-Immunofluorescence-NB100-56546-img0004.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Immunohistochemistry-Paraffin-NB100-56546-img0002.jpg)
![Flow Cytometry: Chk2 Antibody (73C175.1.1) [NB100-56546] - An intracellular stain was performed on Hek293 cells with Chk2 [73C175.1.1] Antibody NB100-56546 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Flow-Cytometry-NB100-56546-img0006.jpg)
![Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Chk2-Antibody-73C175-1-1-Western-Blot-NB100-56546-img0001.jpg)
![Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NB100-1487-img0003.jpg "Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.")
![Simple Western: Rad50 Antibody [NB100-1487] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Rad50-Antibody-Simple-Western-NB100-1487-img0004.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0003.jpg "Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).")
![Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunocytochemistry-Immunofluorescence-NB100-1768-img0005.jpg)
![Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Immunohistochemistry-Paraffin-NB100-1768-img0006.jpg)
![Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using anti-Chk1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Chk1-Antibody-2G1D5-Western-Blot-NB100-1768-img0002.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0021.jpg "Western Blot: MAD2L1 Antibody [NBP1-31311] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MAD2L1 Antibody [NBP1-31311] - Analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31311-img0008.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0013.jpg)
![Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-31311-img0019.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - DLD-1 xenograft, using Mad2L1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0009.jpg)
![Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-31311-img0012.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NBP1-31311-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunocytochemistry-Immunofluorescence-NBP2-43697-img0010.jpg "Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunoprecipitation-NBP2-43697-img0002.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunocytochemistry-immunofluorescence-26112025183418.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-26112025194811.jpg)
![Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251846105.jpg)
![p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.p21 Cip1 stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-immunohistochemistry-paraffin-2611202518315421.jpg)
![p21 Cip1 antibody [GT1032] (NBP2-43697) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT1032] (NBP2-43697) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-flow-cytometry-2611202518321914.jpg)
![Untreated (-) and treated (+) HCT 116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-western-blot-261120251836336.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Staining in human skin and skeletal muscle tissues using anti-SFN antibody. Corresponding SFN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP1-80610-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Staining in human skin and skeletal muscle tissues using anti-SFN antibody. Corresponding SFN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Analysis in human cell lines A-431 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP1-80610-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80610-img0008.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP1-80610-img0006.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP1-80610-img0007.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP1-80610-img0002.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP1-80610] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP1-80610-img0011.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0020.jpg "Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Chk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Chk2.")
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (OTI5C4) [NBP1-47695] - Chk2 Antibody (5C4) [NBP1-47695] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Chk2.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunocytochemistry-Immunofluorescence-NBP1-47695-img0013.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human pancreas tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0019.jpg)
![Flow Cytometry: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of Jurkat cells, using anti-Chk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Flow-Cytometry-NBP1-47695-img0012.jpg)
![Western Blot: Chk2 Antibody (OTI5C4) [NBP1-47695] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Chk2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Western-Blot-NBP1-47695-img0011.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human breast tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0014.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human colon tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0015.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human Kidney tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0016.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human liver tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0017.jpg)
![Immunohistochemistry-Paraffin: Chk2 Antibody (OTI5C4) [NBP1-47695] - Staining of paraffin-embedded Human lung tissue using anti-Chk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Chk2-Antibody-OTI5C4-Immunohistochemistry-Paraffin-NBP1-47695-img0018.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-27072-img0004.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-27072-img0005.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-27072-img0002.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1L4J1-Western-Blot-NBP3-15661-img0010.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] - Analysis of extracts of various cell lines, using p21/CIP1/CDKN1A antibody (NBP3-15661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![<b>Genetic Strategies Validation. </b>Western Blot: p21/CIP1/CDKN1A Antibody (1L4J1) [NBP3-15661] -analysis of lysates from wild type (WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-western-blot-305202492857..jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517344271.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251730319.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-5220251731552.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517361213.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517311090.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (4Z1H9) [p21/CIP1/CDKN1A] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] p21/CIP1/CDKN1A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15661_rabbit-p21-cip1-cdkn1a-mab-4z1h9-52202517352775.jpg)
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NB100-79807-img0011.jpg "Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Detection of human RCH1Importin alpha 2/KPNA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody NB100-79807 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCH1 was also immunoprecipitated by a previous lot of NB100-79807 (lot 1) and by rabbit anti-RCH1 antibody NB100-79808, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79807-img0012.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0012.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunofluorescence-NBP2-52501-img0011.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0009.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0010.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0002.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0003.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunocytochemistry-Immunofluorescence-NBP2-52501-img0006.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0008.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0007.jpg)
![ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-ELISA-NBP2-52501-img0004.jpg)