| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70604 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73604 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38129-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03740-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35353-20ul | |||
![Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/RAD50-Antibody-Immunohistochemistry-Paraffin-NBP2-20055-img0003.jpg "Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.")
![Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 antibody [N1N2], N-term dilution: 1:1000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20055-img0001.jpg)
![Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20055-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Rad50 Antibody [NBP2-20055] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0004.jpg)
![Western Blot: Rad50 Antibody [NBP2-20055] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0005.jpg)
![Western Blot: Rad50 Antibody [NBP2-20055] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0006.jpg)
![Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in JAR.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Western-Blot-H00007150-M01-img0014.jpg "Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in JAR.")
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (1A1) [H00007150-M01] - Analysis of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1~10 ug/ml.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Immunohistochemistry-Paraffin-H00007150-M01-img0011.jpg)
![Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in HeLa.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Western-Blot-H00007150-M01-img0013.jpg)
![Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in human ovarian cancer.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Western-Blot-H00007150-M01-img0015.jpg)
![Immunohistochemistry-Paraffin: Topoisomerase I Antibody (1A1) [H00007150-M01] - Analysis of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-Immunohistochemistry-Paraffin-H00007150-M01-img0009.jpg)
![ELISA: Topoisomerase I Antibody (1A1) [H00007150-M01] - Detection limit for recombinant GST tagged TOP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Topoisomerase-I-Antibody-1A1-ELISA-H00007150-M01-img0016.jpg)
![Western Blot: HERC5 Antibody [H00051191-A01] - Detection against Immunogen (38.21 KDa) .](http://images.novusbio.com/fullsize/HERC5-Antibody-Western-Blot-H00051191-A01-img0001.jpg "Western Blot: HERC5 Antibody [H00051191-A01] - Detection against Immunogen (38.21 KDa) .")
![Western Blot: DTYMK Antibody (1G8) [NBP2-01184] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTYMK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTYMK.](http://images.novusbio.com/fullsize/DTYMK-Antibody-1G8-Western-Blot-NBP2-01184-img0002.jpg "Western Blot: DTYMK Antibody (1G8) [NBP2-01184] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTYMK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTYMK.")
![Immunocytochemistry/Immunofluorescence: DTYMK Antibody (1G8) [NBP2-01184] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DTYMK.](http://images.novusbio.com/fullsize/DTYMK-Antibody-1G8-Immunocytochemistry-Immunofluorescence-NBP2-01184-img0006.jpg)
![Immunohistochemistry-Paraffin: DTYMK Antibody (1G8) [NBP2-01184] - Staining of paraffin-embedded Human prostate tissue using anti-DTYMK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DTYMK-Antibody-1G8-Immunohistochemistry-Paraffin-NBP2-01184-img0005.jpg)
![Flow Cytometry: DTYMK Antibody (1G8) [NBP2-01184] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DTYMK antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/DTYMK-Antibody-1G8-Flow-Cytometry-NBP2-01184-img0001.jpg)
![Immunohistochemistry-Paraffin: DTYMK Antibody (1G8) [NBP2-01184] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DTYMK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DTYMK-Antibody-1G8-Immunohistochemistry-Paraffin-NBP2-01184-img0003.jpg)
![Immunohistochemistry-Paraffin: DTYMK Antibody (1G8) [NBP2-01184] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DTYMK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/DTYMK-Antibody-1G8-Immunohistochemistry-Paraffin-NBP2-01184-img0004.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0007.jpg "Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0001.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0002.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0003.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0004.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0005.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0006.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0008.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of Mouse (A) and Rat (B) Skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0007.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of Mouse (A) and Rat (B) Skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of paraffin embedded human skin. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Image shows strong nuclear staining in selected cells of the basal layer.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NB100-2465-img0004.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - (0.1ug/ml) staining of human skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0003.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of human skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0005.jpg)
![Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of A431 (A) and U2OS (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NB100-2465-img0006.jpg)
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0007.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of Mouse testis, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0008.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (3E9Z3) [Cyclin A2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Cyclin A2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15625_rabbit-cyclin-a2-mab-3e9z3-5220251731556.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (3E9Z3) [Cyclin A2] - Immunohistochemistry analysis of paraffin-embedded Human cervical squamous cell carcinoma using Cyclin A2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15625_rabbit-cyclin-a2-mab-3e9z3-52202517333839.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (3E9Z3) [Cyclin A2] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Cyclin A2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15625_rabbit-cyclin-a2-mab-3e9z3-5220251733575.jpg)
![Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Western blot analysis of extracts of various cell lines, using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Western-Blot-NBP3-16451-img0006.jpg "Western Blot: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Western blot analysis of extracts of various cell lines, using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of NIH-3T3 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunocytochemistry-Immunofluorescence-NBP3-16451-img0005.jpg)
![Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded mouse brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunohistochemistry-Paraffin-NBP3-16451-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunofluorescence analysis of C6 cells using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunocytochemistry-Immunofluorescence-NBP3-16451-img0004.jpg)
![Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded rat brain using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunohistochemistry-Paraffin-NBP3-16451-img0001.jpg)
![Immunohistochemistry-Paraffin: MAD2L2 Antibody (7B6I2) [NBP3-16451] - Immunohistochemistry of paraffin-embedded human colon using MAD2B/MAD2L2 Rabbit mAb (NBP3-16451) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MAD2L2-Antibody-7B6I2-Immunohistochemistry-Paraffin-NBP3-16451-img0002.jpg)
![Immunoprecipitation: RRM2 Antibody [NBP3-38129] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using RRM2 antibody. Western blot was performed from the immunoprecipitate using RRM2 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38129_rabbit-rrm2-pab-91220241581212.jpg "Immunoprecipitation: RRM2 Antibody [NBP3-38129] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using RRM2 antibody. Western blot was performed from the immunoprecipitate using RRM2 antibody at a dilution of 1:1000.")
![Immunohistochemistry: RRM2 Antibody [NBP3-38129] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RRM2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38129_rabbit-rrm2-pab-91220241581248.jpg)
![Immunocytochemistry/ Immunofluorescence: RRM2 Antibody [NBP3-38129] - Immunofluorescence analysis of U2OS cells using RRM2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38129_rabbit-rrm2-pab-912202415152826.jpg)
![Western Blot: RRM2 Antibody [NBP3-38129] - Western blot analysis of lysates from HeLa cells, using RRM2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38129_rabbit-rrm2-pab-912202415121382.jpg)
![Immunohistochemistry: RRM2 Antibody [NBP3-38129] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using RRM2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38129_rabbit-rrm2-pab-912202415175211.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251638096.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of lysates from wild type (WT) and NF2/Merlin knockdown (KD) HeLa cells using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 180 s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: NF2/Merlin Antibody [NBP3-03740] - Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug NF2/Merlin antibody (NBP3-03740). Western blot was performed from the immunoprecipitate using NF2/Merlin antibody (NBP3-03740) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunoprecipitation-NBP3-03740-img0003.jpg)
![Western Blot: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Western blot analysis of various lysates using [KD Validated] NF2/Merlin Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251634529.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP3-03740] - Immunofluorescence analysis of C6 cells using NF2/Merlin antibody (NBP3-03740) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03740-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of U2OS cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-52202516283287.jpg)
![Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody - BSA Free [NF2/Merlin] - Immunofluorescence analysis of L929 cells using [KD Validated] NF2/Merlin Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03740_rabbit-polyclonal-nf2-merlin-antibody-5220251628043.jpg)
![Immunoprecipitation: MCM5 Antibody [NBP3-35353] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411364612.jpg "Immunoprecipitation: MCM5 Antibody [NBP3-35353] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.")
![Western Blot: MCM5 Antibody [NBP3-35353] - Western blot analysis of various lysates using MCM5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411381225.jpg)
![Immunohistochemistry: MCM5 Antibody [NBP3-35353] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411343348.jpg)
![Immunohistochemistry: MCM5 Antibody [NBP3-35353] - Immunohistochemistry analysis of paraffin-embedded Human appendix using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411343375.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-35353] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411334043.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0002.jpg "Western Blot: JWA Antibody [NBP3-12158] - Staining of K562 cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: JWA Antibody [NBP3-12158] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/JWA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12158-img0003.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Small Intestine with antibody at 3.75 g/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0006.jpg)
![Flow Cytometry: JWA Antibody [NBP3-12158] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/JWA-Antibody-Flow-Cytometry-NBP3-12158-img0004.jpg)
![Western Blot: JWA Antibody [NBP3-12158] - Staining of Human Cerebellum lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JWA-Antibody-Western-Blot-NBP3-12158-img0001.jpg)
![Immunohistochemistry-Paraffin: JWA Antibody [NBP3-12158] - Staining of paraffin embedded Human Tonsil with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/JWA-Antibody-Immunohistochemistry-Paraffin-NBP3-12158-img0005.jpg)