| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90369-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89461-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92638-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38210-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20098-0.025mg | |||
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0002.jpg "Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EBP1 Antibody [NBP2-16257] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16257-img0003.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0001.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP2-16257 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-91020242021926.jpg)
![Immunohistochemistry: PA2G4/EBP1 Antibody [NBP2-16257] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PA2G4/EBP1 antibody [N3C3] (NBP2-16257) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-9102024201579.jpg)
![Immunocytochemistry/Immunofluorescence: DRG2 Antibody [NBP1-89461] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DRG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89461-img0006.jpg "Immunocytochemistry/Immunofluorescence: DRG2 Antibody [NBP1-89461] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DRG2 Antibody [NBP1-89461] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DRG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89461-img0007.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)
![Western Blot: DRG2 Antibody - BSA Free [NBP3-46633] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (DRG2 antibody) at dilution of 1:2500](http://images.novusbio.com/fullsize/nbp3-46633_rabbit-drg2-pab-bsa-free-284202511524737.jpg "Western Blot: DRG2 Antibody - BSA Free [NBP3-46633] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (DRG2 antibody) at dilution of 1:2500")
![Immunohistochemistry: DRG2 Antibody - BSA Free [NBP3-46633] - Immunohistochemistry of paraffin-embedded human kidney cancer using (DRG2 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-46633_rabbit-drg2-pab-bsa-free-284202511553136.jpg)
![Immunoprecipitation: DRG2 Antibody - BSA Free [NBP3-46633] - IP Result of anti-DRG2 (IP:, 4ug; Detection: 1:2000) with mouse brain tissue lysate 2640ug.](http://images.novusbio.com/fullsize/nbp3-46633_rabbit-drg2-pab-bsa-free-28420251149222.jpg)
![Western Blot: ERH Antibody (4A10) [H00002079-M14] - Analysis of ERH expression in transfected 293T cell line by ERH monoclonal antibody (M14), clone 4A10. Lane 1: ERH transfected lysatE (12.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-Western-Blot-H00002079-M14-img0006.jpg "Western Blot: ERH Antibody (4A10) [H00002079-M14] - Analysis of ERH expression in transfected 293T cell line by ERH monoclonal antibody (M14), clone 4A10. Lane 1: ERH transfected lysatE (12.3 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ERH Antibody (4A10) [H00002079-M14] - Analysis of monoclonal antibody to ERH on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-Immunocytochemistry-Immunofluorescence-H00002079-M14-img0003.jpg)
![Immunohistochemistry-Paraffin: ERH Antibody (4A10) [H00002079-M14] - Analysis of monoclonal antibody to ERH on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-Immunohistochemistry-Paraffin-H00002079-M14-img0005.jpg)
![ELISA: ERH Antibody (4A10) [H00002079-M14] - Detection limit for recombinant GST tagged ERH is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ERH-Antibody-4A10-ELISA-H00002079-M14-img0002.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Western-Blot-NBP1-71840-img0007.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71840] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- EBP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-NBP1-71840-img0008.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-71839-img0004.jpg "Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).")
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394115..jpg)
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394246..jpg)
![Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ribosomal-protein-S27-Antibody-Western-Blot-NB100-1103-img0003.jpg "Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Ribosomal-protein-S27-Antibody-Immunohistochemistry-Paraffin-NB100-1103-img0001.jpg)
![Western Blot: DRG2 Antibody [NBP2-92638] - Analysis of extracts of various cell lines, using DRG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/DRG2-Antibody-Western-Blot-NBP2-92638-img0002.jpg "Western Blot: DRG2 Antibody [NBP2-92638] - Analysis of extracts of various cell lines, using DRG2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: DRG2 Antibody [NBP2-92638] - Immunofluorescence analysis of L929 cells using DRG2 antibody (NBP2-92638) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DRG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92638-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DRG2 Antibody [NBP2-92638] - Immunofluorescence analysis of C6 cells using DRG2 antibody (NBP2-92638) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DRG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92638-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: NOLC1 Antibody [NBP3-38210] - Immunofluorescence analysis of U-2 OS cells using NOLC1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38210_rabbit-nolc1-pab-1012202411392920.jpg "Immunocytochemistry/ Immunofluorescence: NOLC1 Antibody [NBP3-38210] - Immunofluorescence analysis of U-2 OS cells using NOLC1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: NOLC1 Antibody [NBP3-38210] - Western blot analysis of various lysates using NOLC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38210_rabbit-nolc1-pab-1012202411381296.jpg)
![Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924236.png "Western Blot: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-30C-18 (NBP3-20098) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-30C-18 (NBP3-20098, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-2742023924292.png)
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (SAIC-30C-18) - Azide and BSA Free [NBP3-20098] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30C-18 (NBP3-20098) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20098, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20098_rabbit-rrm2-mab-saic-30c-18-azide-and-bsa-free-274202396465.png)