| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38481-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18565-25ul | |||
| Azide and BSA Free | NBP3-44373 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92397-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57151SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71242 | |||
![Western Blot: EEF1G Antibody [NBP3-38481] - Western blot analysis of various lysates using EEF1G Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38481_rabbit-eef1g-pab-1012202411535862.jpg "Western Blot: EEF1G Antibody [NBP3-38481] - Western blot analysis of various lysates using EEF1G Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunohistochemistry: EEF1G Antibody [NBP3-38481] - Immunohistochemistry analysis of paraffin-embedded Rat brain using EEF1G Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38481_rabbit-eef1g-pab-1012202411545557.jpg)
![Immunocytochemistry/ Immunofluorescence: EEF1G Antibody [NBP3-38481] - Immunofluorescence analysis of NIH-3T3 cells using EEF1G Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38481_rabbit-eef1g-pab-101220241159280.jpg)
![Immunocytochemistry/ Immunofluorescence: EEF1G Antibody [NBP3-38481] - Immunofluorescence analysis of C6 cells using EEF1G Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38481_rabbit-eef1g-pab-1012202411552813.jpg)
![Immunohistochemistry: EEF1G Antibody [NBP3-38481] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using EEF1G Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38481_rabbit-eef1g-pab-10122024121032.jpg)
![Immunohistochemistry: EEF1G Antibody [NBP3-38481] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using EEF1G Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38481_rabbit-eef1g-pab-101220241224112.jpg)
![Western Blot: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipin 1.](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Western-Blot-NBP2-71727-img0001.jpg "Western Blot: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipin 1.")
![Immunohistochemistry: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71727-img0006.jpg)
![Immunohistochemistry: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71727-img0002.jpg)
![Immunohistochemistry: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71727-img0003.jpg)
![Immunohistochemistry: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71727-img0004.jpg)
![Immunohistochemistry: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71727-img0005.jpg)
![Immunohistochemistry: Lipin 1 Antibody (OTI5B5) - Azide and BSA Free [NBP2-71727] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/Lipin-1-Antibody-OTI5B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71727-img0007.jpg)
![Western Blot: GSPT1 Antibody (CL13332) [NBP3-18565] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Western-Blot-NBP3-18565-img0007.jpg "Western Blot: GSPT1 Antibody (CL13332) [NBP3-18565] - Analysis in human cell line K562.")
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0013.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0008.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human ovarian cancer (papillary carcinoma) shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0009.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human colorectal cancer (metastasis to ovary) shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0010.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0011.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13332) [NBP3-18565] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13332-Immunohistochemistry-Paraffin-NBP3-18565-img0012.jpg)
![Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP2-92397-img0004.jpg "Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Rat ovary using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunohistochemistry-Paraffin-NBP2-92397-img0006.jpg)
![Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Mouse lung using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATF5-Antibody-Immunohistochemistry-Paraffin-NBP2-92397-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] - Immunofluorescence analysis of U2OS cells using ATF5 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92397_rabbit-polyclonal-atf5-antibody-42202514275267.jpg)
![Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] - Immunofluorescence analysis of PC-12 cells using ATF5 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92397_rabbit-polyclonal-atf5-antibody-4220251429071.jpg)
![Western Blot: EEF1G Antibody [NB100-2262] - Detection of Human and Mouse EEF1G on HeLa whole cell lystate using NB100-2262.](http://images.novusbio.com/fullsize/EEF1G-Antibody-Western-Blot-NB100-2262-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EEF1G Antibody [NB100-2262] - Detection of Human and Mouse EEF1G on HeLa whole cell lystate using NB100-2262.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: EEF1G Antibody [NB100-2262] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-eEF1G used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EEF1G-Antibody-Immunohistochemistry-NB100-2262-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - GSPT1 antibody [HL1345] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-21202493917.jpg "Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - GSPT1 antibody [HL1345] detects GSPT1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSPT1 stained by GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Western Blot: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-212024939192.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - GSPT1 antibody [HL1345] detects GSPT1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GSPT1 stained by GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-212024940861.jpg)
![Western Blot: GSPT1 Antibody (HL1345) - Azide and BSA Free [NBP3-25492] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GSPT1 antibody [HL1345] (NBP3-25492) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25492_rabbit-gspt1-mab-hl1345-azide-and-bsa-free-2120249403866.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252] - Analysis of extracts of various cell lines, using MKP-3/DUSP6 Rabbit mAb (NBP3-15252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-1V5L10-Western-Blot-NBP3-15252-img0002.jpg "Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252] - Analysis of extracts of various cell lines, using MKP-3/DUSP6 Rabbit mAb (NBP3-15252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252] - Human colon using MKP-3/DUSP6 Rabbit mAb (NBP3-15252) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MKP-3-DUSP6-Antibody-1V5L10-Immunohistochemistry-Paraffin-NBP3-15252-img0001.jpg)
![Immunohistochemistry: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using MKP-3/DUSP6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15252_rabbit-mkp-3-dusp6-mab-1v5l10-52202517162364.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] - Western blot analysis of various lysates using MKP-3/DUSP6 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15252_rabbit-mkp-3-dusp6-mab-1v5l10-52202517251912.jpg)
![Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] - Western blot analysis of various lysates using MKP-3/DUSP6 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15252_rabbit-mkp-3-dusp6-mab-1v5l10-52202517273518.jpg)
![Western Blot: LPIN1 Antibody [NB110-57151] - Detection of LPIN1 in 3T3L1 lysate.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Western-Blot-NB110-57151-img0002.jpg "Western Blot: LPIN1 Antibody [NB110-57151] - Detection of LPIN1 in 3T3L1 lysate.")
![Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Western-Blot-NBP1-47854-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP1-47854-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunohistochemistry-Paraffin-NBP1-47854-img0005.jpg)
![Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP1-51908-img0002.jpg "Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ATF5 Antibody [NBP1-51908] - Analysis of ATF5 antibody in human heart lysate (RIPA buffer, 35 ug total loaded per lane) using NBP1-51908 at 1.5 ug/ml. Primary incubated for 1 hour. ATF5 antibody detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/ATF5-Antibody-Western-Blot-NBP1-51908-img0001.jpg)
![Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-278202416331295.jpg "Western Blot: NFIB Antibody (JE35-21) [NBP3-32647] - Western blot analysis of NFIB on different lysates with Rabbit anti-NFIB antibody (NBP3-32647) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: C6 cell lysate (20 ug/Lane) Lane 3: Human lung tissue lysate (40 ug/Lane) Lane 4: Mouse lung tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32647) at 1/10,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NFIB Antibody (JE35-21) [NBP3-32647] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB antibody (NBP3-32647) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32647) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-298202413585588.jpg)
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody (JE35-21) [NBP3-32647] - Immunocytochemistry analysis of HeLa cells labeling NFIB with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB antibody (NBP3-32647) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32647_rabbit-nfib-mab-je35-21-29202410234232.jpg)
![Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Western-Blot-NBP2-19526-img0002.jpg "Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526] - Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] dilution: 1:500.](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunohistochemistry-Paraffin-NBP2-19526-img0003.jpg)
![Western Blot: NFIB Antibody [NBP2-19526] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIB antibody [N1C2] (NBP2-19526) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19526_rabbit-polyclonal-nfib-antibody-9102024209148.jpg)
![NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] (NBP2-19526) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-19526_rabbit-polyclonal-nfib-antibody-immunohistochemistry-2611202518345220.jpg)