| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76910-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73104 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86099-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87466-25ul | |||
![Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Western-Blot-NBP2-80137-img0008.jpg "Western Blot: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of TDP43 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded human pancreas tissue usingTDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0007.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP-43/TARDBP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0009.jpg)
![Flow Cytometry: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of Hela cells with TDP-43/TARDBP Antibody (JM51-10) at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Flow-Cytometry-NBP2-80137-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Staining TDP-43/TARDBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunocytochemistry-Immunofluorescence-NBP2-80137-img0006.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (JM51-10) [NBP2-80137] - Analysis of paraffin-embedded mouse placenta tissue using TDP-43/TARDBP Antibody (JM51-10). Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-JM51-10-Immunohistochemistry-Paraffin-NBP2-80137-img0003.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0008.jpg "Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575] - Rat brain. APP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP2-15575-img0003.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0004.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0006.jpg)
![Western Blot: APP Antibody [NBP2-15575] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0007.jpg)
![ELISA: APP Antibody [NBP2-15575] - Detection of non-transfected and transfected 293T whole cell extracts using NBP2-15575 as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/APP-Antibody-ELISA-NBP2-15575-img0013.jpg)
![Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NBP1-76910-img0012.jpg "Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419451253.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 ug/mL. Green: APP antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419541571.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419251561.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemical staining of rat brain using APP antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-271120241932669.jpg)
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Western-Blot-NBP2-01086-img0009.jpg "Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunocytochemistry-Immunofluorescence-NBP2-01086-img0008.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0007.jpg)
![Flow Cytometry: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nudel antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Flow-Cytometry-NBP2-01086-img0001.jpg)
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nudel (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nudel.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Western-Blot-NBP2-01086-img0002.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0003.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0004.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Kidney tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0005.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human liver tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0006.jpg)
![Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Western-Blot-NBP3-15677-img0007.jpg "Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0003.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0001.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded human esophageal using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0002.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315540.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517352713.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunoprecipitation of TDP-43/TARDBP from 500 ug extracts of K-562 cells was performed using 2 ug of TDP-43/TARDBP Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TDP-43/TARDBP Rabbit mAb at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-5220251734597.jpg)
![Chromatin Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517344289.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of paraffin-embedded Mouse brain tissue using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517363742.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517361227.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Human colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315511.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517311072.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517304973.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining in human esophagus and pancreas tissues . Corresponding S100A16 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining in human esophagus and pancreas tissues . Corresponding S100A16 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: S100A16 Antibody [NBP1-92361] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-S100A16 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/S100A16-Antibody-Western-Blot-NBP1-92361-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: S100A16 Antibody [NBP1-92361] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92361-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100A16 Antibody [NBP1-92361] - Analysis in human cell lines A-431 and HEK293 using Anti-S100A16 antibody. Corresponding S100A16 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/S100A16-Antibody-Western-Blot-NBP1-92361-img0025.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of glial cells.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0018.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human esophagus shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0019.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0020.jpg)
![Immunohistochemistry-Paraffin: S100A16 Antibody [NBP1-92361] - Staining of human skin shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/S100A16-Antibody-Immunohistochemistry-Paraffin-NBP1-92361-img0021.jpg)
![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining in human epididymis and skeletal muscle tissues using anti-CUL4B antibody. Corresponding CUL4B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining in human epididymis and skeletal muscle tissues using anti-CUL4B antibody. Corresponding CUL4B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0014.jpg)
![Immunohistochemistry-Paraffin: CUL4B Antibody [NBP1-86099] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/CUL4B-Antibody-Immunohistochemistry-Paraffin-NBP1-86099-img0013.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human cerebral cortex, liver, placenta and rectum using Fibrinogen beta chain antibody NBP1-90955 (A) shows similar protein distribution across tissues to independent antibody NBP1-90956 (B).](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90955-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human cerebral cortex, liver, placenta and rectum using Fibrinogen beta chain antibody NBP1-90955 (A) shows similar protein distribution across tissues to independent antibody NBP1-90956 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Fibrinogen beta chain Antibody [NBP1-90955] - Analysis using Fibrinogen beta chain antibody NBP1-90955 (A) shows similar pattern to independent antibody NBP1-90956 (B).](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Western-Blot-NBP1-90955-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human cell line Hep G2 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90955-img0006.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90955-img0011.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90955-img0009.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human placenta shows strong positivity in plasma in blood vessels.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90955-img0010.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90955] - Staining of human rectum shows moderate positivity in plasma in blood vessels, as well as positivity in extracellular matrix.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90955-img0012.jpg)
![Western Blot: Fibrinogen Antibody [NBP2-80414] - Analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Western-Blot-NBP2-80414-img0004.jpg "Western Blot: Fibrinogen Antibody [NBP2-80414] - Analysis of Fibrinogen on mouse bone marrow and human kidney tissue lysates using anti-Fibrinogen antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Fibrinogen Antibody [NBP2-80414] - Staining Fibrinogen in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80414-img0003.jpg)
![Flow Cytometry: Fibrinogen Antibody [NBP2-80414] - Analysis of NIH-3T3 cells with Fibrinogen antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Flow-Cytometry-NBP2-80414-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrinogen Antibody [NBP2-80414] - Staining Fibrinogen in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibrinogen-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80414-img0002.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of dissociated rat hippocampal culture. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-NBP1-87466-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0008.jpg)