| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-78783SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85951-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44243 | |||
| BSA Free | NBP2-61430-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71629 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52501-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96907-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34409-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti-Tensin 1 at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Note the localization of Tensin1 to focal adhesion sites.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78783-img0004.jpg "Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti-Tensin 1 at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Note the localization of Tensin1 to focal adhesion sites.")
![Western Blot: Tensin 1 Antibody [NBP2-78783] - Total protein from human U2OS and HeLa cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tensin1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Western-Blot-NBP2-78783-img0003.jpg)
![Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP2-78783] - Analysis of a FFPE tissue section of the human kidney using 1:200 dilution of Tensin1 antibody (NBP2-78783). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in all cells in glomeruli and tubules.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-78783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP2-78783] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tensin1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tensin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-78783-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP1-85951] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/CSK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85951-img0009.jpg "Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP1-85951] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.")
![Western Blot: CSK Antibody [NBP1-85951] - Analysis in human cell line RT-4 and human cell line HeLa.](http://images.novusbio.com/fullsize/CSK-Antibody-Western-Blot-NBP1-85951-img0010.jpg)
![Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CSK-Antibody-Immunohistochemistry-Paraffin-NBP1-85951-img0013.jpg)
![Immunohistochemistry-Paraffin: CSK Antibody [NBP1-85951] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells and cells in granular layer.](http://images.novusbio.com/fullsize/CSK-Antibody-Immunohistochemistry-Paraffin-NBP1-85951-img0012.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of A549 cells showing specific staining on microtubules in green. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunocytochemistry-Immunofluorescence-NBP2-61430-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunocytochemistry-Immunofluorescence-NBP2-61430-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebellum shows strong positivity in both neurons and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0007.jpg)
![Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Western-Blot-NBP2-37816-img0014.jpg "Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin in SHSY5Y cells (human neuroblast) either left untreated (left panel) or treated with 10uM retinoic acid (right panel) for 72 hours. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37816-img0011.jpg)
![Immunohistochemistry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Normal biopsies of deparaffinized human brain tissues.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunohistochemistry-NBP2-37816-img0012.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody was performed and 10,000 cells were acquired for each sample.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Flow-Cytometry-NBP2-37816-img0010.jpg)
![Immunoprecipitation: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of a beta-3 Tubulin monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. SHSY5Y cell lysate (30ug) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunoprecipitation-NBP2-37816-img0013.jpg)
![Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] Analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Western-Blot-NBP2-03143-img0008.jpg "Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] Analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunocytochemistry-Immunofluorescence-NBP2-03143-img0007.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human prostate tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0006.jpg)
![Western Blot: ALDH1L1 Antibody (3D2) [NBP2-03143] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Western-Blot-NBP2-03143-img0001.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0002.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0003.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human Kidney tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0004.jpg)
![Immunohistochemistry-Paraffin: ALDH1L1 Antibody (3D2) [NBP2-03143] - Staining of paraffin-embedded Human liver tissue using anti-ALDH1L1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-3D2-Immunohistochemistry-Paraffin-NBP2-03143-img0005.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0012.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunofluorescence-NBP2-52501-img0011.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0009.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0010.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0002.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0003.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunocytochemistry-Immunofluorescence-NBP2-52501-img0006.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0008.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0007.jpg)
![ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-ELISA-NBP2-52501-img0004.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0003.jpg "Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Flow Cytometry: c-jun Antibody (5B1) [NBP2-37576] - Analysis of HepG2 cells using c-Jun mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Flow-Cytometry-NBP2-37576-img0005.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mAb against human c-Jun (AA: 199-331) recombinant protein.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Western-Blot-NBP2-37576-img0002.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human intima cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-Immunohistochemistry-NBP2-37576-img0006.jpg)
![ELISA: c-jun Antibody (5B1) [NBP2-37576] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-jun-Antibody-5B1-ELISA-NBP2-37576-img0001.jpg)
![Western Blot: p38 alpha Antibody [NB110-96907] - Western blot analysis of Human cancer cell lines showing detection of p38 alpha protein using Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Western-Blot-NB110-96907-img0008.jpg "Western Blot: p38 alpha Antibody [NB110-96907] - Western blot analysis of Human cancer cell lines showing detection of p38 alpha protein using Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 alpha Polyclonal Antibody (NB110-96907) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody [NB110-96907] - Analysis using Rabbit Anti-p38 Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96907-img0007.jpg)
![Western Blot: p38 alpha Antibody [NB110-96907] - CtsK & NFATc1 expression levels were decreased during osteoclastogenesis by PSTP-3,5-Me treatment. (A) mRNA expression levels of osteoclast-specific markers were determined by RT-PCR in 0 or 6 µM PSTP-3,5-Me-treated cells. *p < 0.05, & **p < 0.01 indicate the statistically significant difference between non-PSTP-3,5-Me-treated groups (0 µM) & PSTP-3,5-Me-treated groups (6 µM) on each day. Mean ± standard error. (B) Western blotting was performed to determine phosphorylation of NF-kB, p-38, ERK1/2, & JNK. Cells were pre-treated with 6 µM PSTP-3,5-Me or vehicle (Ctrl) for 2 h, & then treated with RANKL for the indicated times. beta -Actin expression level was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31540026), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96907_rabbit-polyclonal-p38-alpha-antibody-31020241534327.jpg)
![Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Western-Blot-NB100-58788-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cullin 3 Antibody [NB100-58788] - Whole cell lysate (1 mg/IP, 20% IP loaded) HeLa cells. NB100-58788 used for IP(3 ug/mg lysate). Cul3 also immunoprecipitated by other rabbit anti-Cul3 antibodies(recognize downstream epitopes).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Cullin 3 Antibody [NB100-58788] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cul3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cullin-3-Antibody-Immunohistochemistry-NB100-58788-img0003.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)