| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22832SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26645-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26167-50ul | |||
![Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-22832-img0004.jpg "Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.")
![Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP1-33474-img0004.jpg "Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-33474] - Paraformaldehyde-fixed HeLa, using CLIC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33474-img0002.jpg)
![Western Blot: CLIC4 Antibody [NBP1-33474] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CLIC4-Antibody-Western-Blot-NBP1-33474-img0003.jpg)
![Immunohistochemistry: CLIC4 Antibody [NBP1-33474] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CLIC4 antibody (NBP1-33474) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-33474_rabbit-polyclonal-clic4-antibody-91020242063384.jpg)
![Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (NBP1-33474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33474_rabbit-polyclonal-clic4-antibody-910202420104446.jpg)
![Immunohistochemistry: Somatostatin R2/SSTR2 Antibody (1C3) [NBP3-15215] - IHC image of Somatostatin R2/SSTR2 antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Somatostatin-R2-SSTR2-Antibody-1C3-Immunohistochemistry-NBP3-15215-img0001.jpg "Immunohistochemistry: Somatostatin R2/SSTR2 Antibody (1C3) [NBP3-15215] - IHC image of Somatostatin R2/SSTR2 antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NB100-61587-img0004.jpg "Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.")
![Immunohistochemistry-Paraffin: HDAC7 Antibody [NB100-61587] - Human prostate carcinoma (left) and mouse colon carcinoma (right). Antibody used at a dilution of 1:5,000.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunohistochemistry-Paraffin-NB100-61587-img0003.jpg)
![Immunohistochemistry-Paraffin: TUBGCP2 Antibody [NBP2-53097] - Analysis of paraffin-embedded human hepatoma, using GCP2 antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/TUBGCP2-Antibody-Immunohistochemistry-Paraffin-NBP2-53097-img0002.jpg "Immunohistochemistry-Paraffin: TUBGCP2 Antibody [NBP2-53097] - Analysis of paraffin-embedded human hepatoma, using GCP2 antibody at 1:500 dilution.")
![GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis. <BR/>Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.<BR/>Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term (NBP2-53097) diluted at 1:500.<BR/>Blue: Hoechst 33342 staining.<BR/>[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]](http://images.novusbio.com/fullsize/nbp2-53097_rabbit-polyclonal-tubgcp2-antibody-immunocytochemistry-immunofluorescence-261120251830123.jpg)
![Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ACTR1B-Antibody-Western-Blot-NBP2-15275-img0002.jpg "Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-15275] - Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ACTR1B-Antibody-Immunohistochemistry-Paraffin-NBP2-15275-img0003.jpg)
![Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ACTR1B-Antibody-Western-Blot-NBP2-15275-img0001.jpg)
![Western Blot: ANKRD28 Antibody [NB100-41419] - Whole cell lysate from HeLa cells (5, 15 and 50 mcg for WB; 1 mg for IP, 20 percent of IP loaded) and 293T (T; 50 mcg) cells. NB100-41419 used for WB at 0.02 mug/ml (A) and 1 mug/ml (B) and used for IP at 3 mcg/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody NB100-41420, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/ANKRD28-Antibody-Western-Blot-NB100-41419-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ANKRD28 Antibody [NB100-41419] - Whole cell lysate from HeLa cells (5, 15 and 50 mcg for WB; 1 mg for IP, 20 percent of IP loaded) and 293T (T; 50 mcg) cells. NB100-41419 used for WB at 0.02 mug/ml (A) and 1 mug/ml (B) and used for IP at 3 mcg/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody NB100-41420, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: ANKRD28 Antibody [NB100-41419] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/ANKRD28-Antibody-Immunohistochemistry-NB100-41419-img0005.jpg)
![Immunohistochemistry-Paraffin: ANKRD28 Antibody [NB100-41419] - FFPE section of human stomach carcinoma. Antibody used at a dilution of 1:250.](http://images.novusbio.com/fullsize/ANKRD28-Antibody-Immunohistochemistry-Paraffin-NB100-41419-img0001.jpg)
![Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-246-img0002.jpg)
![Immunohistochemistry: COP9 Antibody (3F9) [NBP3-26645] - Image of COP9 Antibody (3F9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-26220241422527.jpg "Immunohistochemistry: COP9 Antibody (3F9) [NBP3-26645] - Image of COP9 Antibody (3F9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: COP9 Antibody (3F9) [NBP3-26645] - Image of COP9 Antibody (3F9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-26220241535576.jpg)
![Western Blot: COP9 Antibody (3F9) [NBP3-26645] - Positive Western Blot detected in: U251 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate.<br /> All lanes: COP9 Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 48, 46 kDa<br /> Observed band size: 40-55 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-282202411472142.jpg)
![Immunocytochemistry/Immunofluorescence: COP9 Antibody (3F9) [NBP3-26645] - Staining of A549 cell with COP9 Antibody (3F9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 535-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-2822024132364.jpg)
![Flow Cytometry: COP9 Antibody (3F9) [NBP3-26645] - Overlay Peak curve showing MCF7 cells stained with COP9 Antibody (3F9) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26645_rabbit-cop9-mab-3f9-282202414312713.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug).<br /> Lane 3: Hela whole cell lysate (20ug).<br />](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202413221512.jpg "Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug).<br /> Lane 3: Hela whole cell lysate (20ug).<br />")
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202414471576.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-2722024820395.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4H7) [NBP3-26167] - Staining of Hela cells with Lamin A + C Antibody (4H7) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202412493637.jpg)
![Flow Cytometry: Lamin A + C Antibody (4H7) [NBP3-26167] - Overlay histogram showing Hela cells stained with Lamin A + C Antibody (4H7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.</p>](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202414312758.jpg)
![Western Blot: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (5, 15, and 50 ug) stained with CEP170 Antibody diluted at 0.04 ug/ml.](http://images.novusbio.com/fullsize/CEP170-Antibody-Western-Blot-NB100-55317-img0002.jpg "Western Blot: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (5, 15, and 50 ug) stained with CEP170 Antibody diluted at 0.04 ug/ml.")
![Immunocytochemistry/Immunofluorescence: CEP170 Antibody [NB100-55317] - NBF-fixed asynchronous HeLa cells, stained wtih CEP170 Antibody diluted at 1:200. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus(TM) 555.](http://images.novusbio.com/fullsize/CEP170-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55317-img0001.jpg)
![Immunoprecipitation: CEP170 Antibody [NB100-55317] - Hela whole cell lysate (1 mg) immunoprecipitated by rabbit anti-CEPT170 antibodies NB100-55317 at 3 ug/mg lysate.CEP170 was also immunoprecipitated by rabbit anti-CEP170 antibodies NB100-55317 and BL4463, which recognize upstream epitopes.](http://images.novusbio.com/fullsize/CEP170-Antibody-Immunoprecipitation-NB100-55317-img0003.jpg)