| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03559-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-35293-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04398-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35749-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56441SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50054SS | |||
![Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Western-Blot-NBP2-46199-img0006.jpg "Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.")
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0005.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0001.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0002.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0003.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0004.jpg)
![Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Western-Blot-NBP1-06076-img0002.jpg "Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Breast tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0003.jpg)
![Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Tonsil tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-06076-img0004.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0004.jpg "Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Brain, Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry-Paraffin: Septin-11 Antibody [NBP1-58144] - Human Spleen Tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Immunohistochemistry-Paraffin-NBP1-58144-img0003.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - 1. Human NT-2 cells (60ug) 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody : IRDye 800CW goat anti-rabbit Secondary Antibody Dilution: 1: 20,000](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0001.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - NT-2 cell line, mouse brain, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0002.jpg)
![Western Blot: Septin-11 Antibody [NBP1-58144] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-58144-img0005.jpg)
![Western Blot: PARD6A Antibody [NB300-953] - (2ug/ml) staining of Jurkat (A) and U251 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PARD6A-Antibody-Western-Blot-NB300-953-img0006.jpg "Western Blot: PARD6A Antibody [NB300-953] - (2ug/ml) staining of Jurkat (A) and U251 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953] - (10ug/ml) staining of paraffin embedded Human Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/PARD6A-Antibody-Immunohistochemistry-Paraffin-NB300-953-img0005.jpg)
![Flow Cytometry: PARD6A Antibody [NB300-953] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PARD6A-Antibody-Flow-Cytometry-NB300-953-img0007.jpg)
![Immunohistochemistry-Paraffin: PARD6A Antibody [NB300-953] - (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PARD6A-Antibody-Immunohistochemistry-Paraffin-NB300-953-img0004.jpg)
![Western Blot: MLL5 Antibody [NBP3-03559] - Western blot analysis of extracts of C2C12 cells, using (NBP3-03559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MLL5 Antibody-Western Blot-NBP3-03559-img0005.jpg "Western Blot: MLL5 Antibody [NBP3-03559] - Western blot analysis of extracts of C2C12 cells, using (NBP3-03559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry: MLL5 Antibody [NBP3-03559] - Analysis of mouse brain using MLL5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MLL5-Antibody-Immunohistochemistry-NBP3-03559-img0001.jpg)
![Immunohistochemistry: MLL5 Antibody - Azide and BSA Free [MLL5] - Immunohistochemistry analysis of paraffin-embedded Rat lung using MLL5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03559_rabbit-polyclonal-mll5-antibody-52202516321453.jpg)
![Immunohistochemistry: MLL5 Antibody - Azide and BSA Free [MLL5] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using MLL5 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03559_rabbit-polyclonal-mll5-antibody-5220251639636.jpg)
![Immunohistochemistry: Septin-5 Antibody [NBP3-35293] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Septin-5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-10122024122413.jpg "Immunohistochemistry: Septin-5 Antibody [NBP3-35293] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Septin-5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: Septin-5 Antibody [NBP3-35293] - Western blot analysis of various lysates, using Septin-5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-10122024125287.jpg)
![Immunohistochemistry: Septin-5 Antibody [NBP3-35293] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Septin-5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-101220241214467.jpg)
![Immunocytochemistry/ Immunofluorescence: Septin-5 Antibody [NBP3-35293] - Immunofluorescence analysis of U-2 OS cells using Septin-5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-1012202411531073.jpg)
![Western Blot: KLHL9 Antibody [NBP3-04398] - Analysis of extracts of 293T cells, using KLHL9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/KLHL9-Antibody-Western-Blot-NBP3-04398-img0005.jpg "Western Blot: KLHL9 Antibody [NBP3-04398] - Analysis of extracts of 293T cells, using KLHL9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .")
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded mouse testis using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0004.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded rat ovary using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0001.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded rat heart using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0002.jpg)
![Immunohistochemistry-Paraffin: KLHL9 Antibody [NBP3-04398] - Paraffin-embedded human breast cancer using KLHL9 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KLHL9-Antibody-Immunohistochemistry-Paraffin-NBP3-04398-img0003.jpg)
![Western Blot: Septin-1 Antibody [NBP3-35749] - Western blot analysis of various lysates, using Septin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411343345.jpg "Western Blot: Septin-1 Antibody [NBP3-35749] - Western blot analysis of various lysates, using Septin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Western Blot: Septin-1 Antibody [NBP3-35749] - Western blot analysis of lysates from Rat spleen, using Septin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-101220241136056.jpg)
![Immunohistochemistry: Septin-1 Antibody [NBP3-35749] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Septin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411334077.jpg)
![Immunohistochemistry: Septin-1 Antibody [NBP3-35749] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Septin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411343372.jpg)
![Immunohistochemistry: Septin-1 Antibody [NBP3-35749] - Immunohistochemistry analysis of paraffin-embedded Human appendix using Septin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35749_rabbit-septin-1-pab-1012202411353057.jpg)
![Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-278202415242619.jpg "Western Blot: KIFC1 Antibody (JE00-46) [NBP3-32504] - Western blot analysis of KIFC1 on different lysates with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32504) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32504) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-298202414262691.jpg)
![Immunocytochemistry/ Immunofluorescence: KIFC1 Antibody (JE00-46) [NBP3-32504] - Immunocytochemistry analysis of Jurkat cells labeling KIFC1 with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KIFC1 antibody (NBP3-32504) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202410334073.jpg)
![Flow Cytometry: KIFC1 Antibody (JE00-46) [NBP3-32504] - Flow cytometric analysis of Jurkat cells labeling KIFC1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32504, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32504_rabbit-kifc1-mab-je00-46-29202416453263.jpg)
![Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.](http://images.novusbio.com/fullsize/EB3-Antibody-Western-Blot-NB100-56441-img0002.jpg "Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.")
![Western Blot: Septin-11 Antibody [NBP1-31717] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-11 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-31717-img0009.jpg "Western Blot: Septin-11 Antibody [NBP1-31717] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-11 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Septin-11 Antibody [NBP1-31717] - Sample: Paraffin-embedded hepatoma. Septin-11 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Immunohistochemistry-Paraffin-NBP1-31717-img0006.jpg)
![Western Blot: Septin-11 Antibody [NBP1-31717] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-31717-img0004.jpg)
![Western Blot: Septin-11 Antibody [NBP1-31717] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-11 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-31717-img0007.jpg)
![Western Blot: Septin-11 Antibody [NBP1-31717] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-11 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Septin-11-Antibody-Western-Blot-NBP1-31717-img0008.jpg)
![Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Western-Blot-NBP2-50054-img0004.jpg "Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - Analysis of HeLa cells stained with mouse mAb to aurora B kinase, NBP2-50054, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. NBP2-50054 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - HeLa cell cultures were stained with NBP2-50054 (green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0003.jpg)