| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92967-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44730 | |||
| Azide and BSA Free | NBP2-34767-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26609-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47835-0.1mg | |||
| IHC-Prediluted | NBP2-48284 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54476-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-07903 | |||
![Western Blot: Cdk6 Antibody [NBP2-92967] - analysis of lysates from HeLa cells, using CDK6 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25Ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92967_rabbit-polyclonal-cdk6-antibody-western-blot-1542024133214..jpg "Western Blot: Cdk6 Antibody [NBP2-92967] - analysis of lysates from HeLa cells, using CDK6 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25Ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Geminin Antibody [NBP2-16639] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16639-img0002.jpg "Immunocytochemistry/Immunofluorescence: Geminin Antibody [NBP2-16639] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-16639] - Detects Geminin protein at nucleus on breast carcinoma.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunohistochemistry-Paraffin-NBP2-16639-img0003.jpg)
![Immunoprecipitation: Geminin Antibody [NBP2-16639] - HCT-116 whole cell extracts using 5 ug of Geminin antibody. Western blot analysis was performed using Geminin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunoprecipitation-NBP2-16639-img0004.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NBP1-31098-img0008.jpg "Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Importin alpha 2/KPNA2 Antibody [NBP1-31098] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA2 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-NBP1-31098-img0011.jpg)
![karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term (NBP1-31098) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-31098_rabbit-polyclonal-importin-alpha-2-kpna2-antibody-immunocytochemistry-immunofluorescence-141120251844345.jpg)
![Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0005.jpg "Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (339) [NB100-478] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using 1:500 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Immunocytochemistry-Immunofluorescence-NB100-478-img0002.jpg)
![Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat , B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0003.jpg)
![Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PCNA-Antibody-339-Western-Blot-NB100-478-img0004.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![<b>Independent Antibodies Validation. </b>Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).](http://images.novusbio.com/fullsize/PCNA-Antibody-SPM350-Immunohistochemistry-Paraffin-NBP2-32989-img0002.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (SPM350) [NBP2-32989] - Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Antibody (SPM350).")
![Western Blot: CIRBP Antibody [NBP2-15905] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CIRBP-Antibody-Western-Blot-NBP2-15905-img0001.jpg "Western Blot: CIRBP Antibody [NBP2-15905] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CIRBP Antibody [NBP2-15905] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIRBP protein stained by CIRBP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CIRBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15905-img0003.jpg)
![Immunohistochemistry-Paraffin: CIRBP Antibody [NBP2-15905] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CIRBP-Antibody-Immunohistochemistry-Paraffin-NBP2-15905-img0002.jpg)
![Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-262202414471553.jpg "Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-27220248471914.jpg)
![Western Blot: E2F-1 Antibody (1D12) [NBP3-26609] - Positive Western Blot detected in: Jurkat whole cell lysate, PC-3 whole cell lysate, Rat Heart tissue. All lanes: E2F-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-2822024115259.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (1D12) [NBP3-26609] - Staining of Hela Cells with E2F-1 Antibody (1D12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-282202412493640.jpg)
![Flow Cytometry: E2F-1 Antibody (1D12) [NBP3-26609] - Overlay histogram showing Hela cells stained with E2F-1 Antibody (1D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-282202414312778.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0004.jpg "Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cdk6 Antibody [NBP2-15846] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0001.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0002.jpg)
![CDK6 antibody [N3C3] detects CDK6 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK6 protein stained by CDK6 antibody [N3C3] (NBP2-15846) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15846_rabbit-polyclonal-cdk6-antibody-immunocytochemistry-immunofluorescence-2611202518395722.jpg)
![Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Western-Blot-NBP2-44731-img0002.jpg "Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)")
![Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) [NBP2-44731] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Immunohistochemistry-Paraffin-NBP2-44731-img0001.jpg)
![Flow Cytometry: PCNA Antibody (PCNA/694) [NBP2-44731] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PCNA-Antibody-PCNA-694-Flow-Cytometry-NBP2-44731-img0003.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0002.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).")
![Immunohistochemistry-Paraffin: RB1 Antibody (RB1/1754) [NBP2-53400] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).](http://images.novusbio.com/fullsize/RB1-Antibody-RB1-1754-Immunohistochemistry-Paraffin-NBP2-53400-img0001.jpg)
![Western Blot: E-Cadherin Antibody (CDH1/2208R) [NBP3-07902] - Western Blot Analysis of MCF-7 cells using E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-2208R-Western-Blot-NBP3-07902-img0002.jpg "Western Blot: E-Cadherin Antibody (CDH1/2208R) [NBP3-07902] - Western Blot Analysis of MCF-7 cells using E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CDH1/2208R) [NBP3-07902] - Immunofluorescence Analysis of MCF-7 cells labeling CHD1 with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-2208R-Immunocytochemistry-Immunofluorescence-NBP3-07902-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CDH1/2208R) [NBP3-07902] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-2208R-Immunohistochemistry-Paraffin-NBP3-07902-img0005.jpg)
![Flow Cytometry: E-Cadherin Antibody (CDH1/2208R) [NBP3-07902] - Flow Cytometric Analysis of MCF-7 cells using E-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH1/2208R) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CDH1-2208R-Flow-Cytometry-NBP3-07902-img0004.jpg)