| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54153SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44677 | |||
| Azide and BSA Free | NBP2-34772-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86626-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85393-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87327-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71242 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70286 | |||
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyoD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0005.jpg "Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.](http://images.novusbio.com/fullsize/MyoD1-Antibody-Western-Blot-NBP1-54153-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MyoD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0003.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0010.jpg "Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Co-localization of necdin and CCAR1 in primary myoblasts of wt (C57/Bl6) mice. Images taken at confocal laser scanning microscope at 40x (i-v) and 63x (zoomed 1.67 times) magnification. Nuclei are stained with DAPI (i, vi; blue). Panels shows immunostaining experiment using the polyclonal anti-CCAR1 (ii, vii, Ab-CCAR1-red) or monoclonal anti-Ndn (iii, viii; Ab-Ndn-green). Panel iv shows the merged images of ii and iii and panel ix the merged images of vii and viii (Ab-CCAR1 + Ab-Ndn-yellow). Panel v shows the merged images of i, ii and iii and panel x the merged images of vi, vii, viii, (Ab-CCAR + Ab-Ndn + DAPI). Scale bar (i-iv) = 17 um; (vi-x) = 41,75 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0011.jpg)
![Immunohistochemistry-Paraffin: CCAR1 Antibody [NB500-186] - Human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-CCAR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunohistochemistry-Paraffin-NB500-186-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human CCAR1on HeLa whole cell lysate using NB500-186. CCAR1 was also IPed with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. IPed CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human and Mouse CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0006.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Interaction of necdin and CCAR1 in C2C12 myoblasts and primary myoblast cells. Co-IP experiments were performed on protein extract from C2C12 transfected with pCMV-FLAG-Necdin (C2C12 pCMV-FLAG-Ndn) or primary myoblasts from C57/Bl6 newborn mice. Proteins were immunoprecipitated using the polyclonal anti-Ndn (alpha-Ndn) or polyclonal anti-CCAR1 (alpha-CCAR1) and with a non-specific rabbit antisera as control (IgG). Necdin and CCAR1 were detected in immunoprecipitated samples using specific antibody. Input (I) represents 10% of the immunoprecipitated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0008.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing CCAR1 and necdin (beta-actin as loading control) expression in C2C12 mock transfected (M) or transfected with pSG5-HA-CCAR1 and/or pCMV-FLAG-Necdin (N or N+C). Both the endogenous and transfected CCAR1 were detected with the polyclonal anti-CCAR1, the transfected CCAR1 with anti-HA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Images taken at confocal laser scanning microscope (63x magnif. zoomed 1.67 x) showing co-immunostaining on C2C12 transfected with pSG5-HA-CCAR1 (i) and/or pCMV-Ndn (C2C12, CCAR1-Ndn-N+C) (ii, iii) and on primary myoblasts from Ndn-/- (Ndn-/-) (iv) and tgMlcNec (MlcNec) (v) newborn mice. Panels i-v show immunostaining using the specific monoclonal anti-Ndn (Ndn: Ab-Ndn-green); panels i'-v' show immunostaining using the polyclonal anti-CCAR1 (CCAR1: Ab-CCAR1-red). Co-immunostained images of anti-Ndn and anti-CCAR1 are shown in panels i''-v'' (Ab-CCAR1 + Ab-Ndn-yellow). Panels i'''-v''' show merged images with nuclei stained with DAPI (merge Ab-CCAR1 + ab-Ndn + DAPI). Scale bars (i-iii) 41,75 um; (iv-v) 44,7 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0012.jpg)
![Immunoprecipitation: CCAR1 Antibody [NB500-186] - Detection of human CCAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 (lot NB500-186-2) used for IP at 6 ug/mg lysate. CCAR1 was also immunoprecipitated by a previous lot (lot NB500-186-1) of the antibody. For blotting immunoprecipitated CCAR1, NB500-186 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunoprecipitation-NB500-186-img0007.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416182617.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416165639.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-24679-img0002.jpg "Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.")
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)
![Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).](http://images.novusbio.com/fullsize/MyoD-Antibody-SPM427-Immunohistochemistry-Paraffin-NBP2-32882-img0003.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).")
![Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-212024939119.jpg "Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunofluorescent analysis. Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-2120249383179.jpg)
![Immunohistochemistry-Paraffin: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:100. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-212024938313.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MyoD antibody [HL1372] (NBP3-25573) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-western-blot-1411202518243310.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD antibody [HL1372] (NBP3-25573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-western-blot-1411202518334516.jpg)
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592116.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 3. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420594212.jpg)
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 2. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-81120242059212.jpg)
![Western Blot: MyoD Antibody (5.2F) [NBP3-30815] - Left: Immunohistochemical staining using MyoD antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Right: Western blot using 60 ng of purified MyoD protein with MyoD antibody at 2 ug/ml and detected using goat anti-mouse HRP and visualized with Pierce West-Femto substrate.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592184.jpg)
![Immunohistochemistry-Paraffin: CCAR1 Antibody [NBP1-86626] - Staining of human lymph node shows strong nuclear positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunohistochemistry-Paraffin-NBP1-86626-img0007.jpg "Immunohistochemistry-Paraffin: CCAR1 Antibody [NBP1-86626] - Staining of human lymph node shows strong nuclear positivity in germinal center and non-germinal center cells.")
![Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Analysis in human testis and skeletal muscle tissues using NBP1-85393 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunohistochemistry-Paraffin-NBP1-85393-img0007.jpg "Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Analysis in human testis and skeletal muscle tissues using NBP1-85393 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.")
![Immunocytochemistry/Immunofluorescence: SPAG9 Antibody [NBP1-85393] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85393-img0006.jpg)
![Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunohistochemistry-Paraffin-NBP1-85393-img0010.jpg)
![Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunohistochemistry-Paraffin-NBP1-85393-img0008.jpg)
![Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Lymph node shows very weak cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunohistochemistry-Paraffin-NBP1-85393-img0009.jpg)
![Immunohistochemistry-Paraffin: SPAG9 Antibody [NBP1-85393] - Staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunohistochemistry-Paraffin-NBP1-85393-img0011.jpg)
![Western Blot: SPAG9 Antibody [NB100-79794] - Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50 ug), Renca (50 ug), and CT26 (50 ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Western-Blot-NB100-79794-img0007.jpg "Western Blot: SPAG9 Antibody [NB100-79794] - Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50 ug), Renca (50 ug), and CT26 (50 ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: SPAG9 Antibody [NB100-79794] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunohistochemistry-NB100-79794-img0004.jpg)
![Western Blot: SPAG9 Antibody [NB100-79794] - Nuclear extract (NE, 20 ug) from HeLa cells. Antibody used at 0.3 ug/ml.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Western-Blot-NB100-79794-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SPAG9 Antibody [NB100-79794] - Detection of Human JLP by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and 293T (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Western-Blot-NB100-79794-img0006.jpg)
![Immunoprecipitation: SPAG9 Antibody [NB100-79794] - Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 (lot NB100-79794-2) used for IP at 6 ug/mg lysate. JLP was also immunoprecipitated by a previous lot (NB100-79794-1) of this antibody. For blotting immunoprecipitated JLP, NB100-79794 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SPAG9-Antibody-Immunoprecipitation-NB100-79794-img0008.jpg)
![Western Blot: IFRD1 Antibody [NBP1-87327] - Analysis in control (vector only transfected HEK293T lysate) and IFRD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP1-87327-img0006.jpg "Western Blot: IFRD1 Antibody [NBP1-87327] - Analysis in control (vector only transfected HEK293T lysate) and IFRD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: IFRD1 Antibody [NBP1-87327] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87327-img0005.jpg)
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP1-87327] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP1-87327-img0002.jpg)
![Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Western-Blot-NBP1-47854-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP1-47854-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunohistochemistry-Paraffin-NBP1-47854-img0005.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0004.jpg "Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0001.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0002.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0003.jpg)
![Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/BTG1-Antibody-7H6-Immunohistochemistry-NBP2-46586-img0005.jpg)