| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86635-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92161-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38191-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88346-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27148-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81615-25ul | |||
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88345-img0017.jpg "Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining in human testis and kidney tissues . Corresponding NCAPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0016.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0014.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88345 (A) shows similar protein distribution across tissues to independent antibody NBP1-88346 (B).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human skin shows nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human tonsil shows nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0015.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0006.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0007.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86635-img0008.jpg "Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP1-86635-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86635_rabbit-polyclonal-smc4-antibody-30420251371429.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Western blot analysis of MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, and 15.5 dpp and adult mice. Each lane was loaded with 50 ug of testis protein extracts. Alpha tubulin was used as internal control. Image collected and cropped by CiteAb from the following publication (//link.springer.com/article/10.1007/s00412-017-0631-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-H00010445-B01P-img0007.jpg "Western Blot: MCRS1 Antibody [H00010445-B01P] - Western blot analysis of MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, and 15.5 dpp and adult mice. Each lane was loaded with 50 ug of testis protein extracts. Alpha tubulin was used as internal control. Image collected and cropped by CiteAb from the following publication (//link.springer.com/article/10.1007/s00412-017-0631-z) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunocytochemistry-Immunofluorescence-H00010445-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: MCRS1 Antibody [H00010445-B01P] - Analysis of purified antibody to MCRS1 on formalin-fixed paraffin-embedded human spleen. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunohistochemistry-Paraffin-H00010445-B01P-img0005.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Analysis of MCRS1 expression in transfected 293T cell line by MCRS1 polyclonal antibody. Lane 1: MCRS1 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-H00010445-B01P-img0006.jpg)
![Western Blot: MCRS1 Antibody [H00010445-B01P] - Identification of interacting partners of PRDM9 by Y2H screening. a Schematic representation of the domain structure of seven PRDM9-interacting proteins identified by Y2H screening. The prey fragments identified in the screens are shown as blue bars underneath each protein. The length (number of amino acid residues) of each full-length protein is indicated on the right. b RNA expression of the PRDM9-interacting proteins in mouse testes (left) & ovaries (right) during meiosis. Data were extracted from RNA-seq data in Margolin et al. (2014) & Soh et al. (2015)). The average gene expression is shown in reads per kilobase per million of reads (RPKM) & fragments per kilobase of transcript per million mapped read (FKPM) for testes & ovaries, respectively. Zoomed-in views of the dashed line boxes are shown below. Spo11 & Spata19 expression were used as positive & negative controls, respectively. c Western blot analysis of PRDM9, CXXC1, PIH1D1, CEP70, & MCRS1 expression in whole testis extracts from 4.5, 6.5, 9.5, 12.5, & 15.5 dpp & adult mice. Each lane was loaded with 50 μg of testis protein extracts. Tubulin alpha was used as internal control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28527011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010445-b01p_mouse-polyclonal-mcrs1-antibody-310202415483646.jpg)
![Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP1-92161-img0005.jpg "Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunocytochemistry/Immunofluorescence: HBO1 Antibody [NBP1-92161] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92161-img0007.jpg)
![Immunohistochemistry-Paraffin: HBO1 Antibody [NBP1-92161] - Staining of human stomach shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HBO1-Antibody-Immunohistochemistry-Paraffin-NBP1-92161-img0002.jpg)
![Western Blot: HBO1 Antibody [NBP1-92161] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/HBO1-Antibody-Western-Blot-NBP1-92161-img0004.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-92161_rabbit-polyclonal-hbo1-antibody-295202516464821.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411421032.jpg "Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411421073.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411343384.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-101220241136061.jpg)
![Immunohistochemistry: HBO1 Antibody [NBP3-38191] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using HBO1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-101220241136069.jpg)
![Western Blot: HBO1 Antibody [NBP3-38191] - Western blot analysis of various lysates using HBO1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38191_rabbit-hbo1-pab-1012202411364661.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88346 (A) shows similar protein distribution across tissues to independent antibody NBP1-88345 (B).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88346-img0015.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88346 (A) shows similar protein distribution across tissues to independent antibody NBP1-88345 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining in human testis and kidney tissues . Corresponding NCAPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88346-img0012.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human testis shows nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88346-img0010.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88346-img0008.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human skin shows nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88346-img0009.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88346] - Staining of human tonsil shows nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88346-img0011.jpg)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491704.jpg%3Fv=1666704274 "Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: HBO1 Antibody (1D9H9) [NBP3-27148] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916212.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded cervical cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202492021.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024916234.jpg%3Fv=1666704274)
![Western Blot: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-1042024917033.jpg%3Fv=1666704274)
![Immunohistochemistry: HBO1 Antibody (1D9H9) [NBP3-27148] - Analysis of paraffin-embedded colon cancer tissues using HBO1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27148_mouse-hbo1-mab-1d9h9-bsa-free-104202491629.jpg%3Fv=1666704274)
![Western Blot: MCRS1 Antibody [NBP1-81615] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-NBP1-81615-img0007.jpg "Western Blot: MCRS1 Antibody [NBP1-81615] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: MCRS1 Antibody [NBP1-81615] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81615-img0009.jpg)
![Immunohistochemistry-Paraffin: MCRS1 Antibody [NBP1-81615] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/MCRS1-Antibody-Immunohistochemistry-Paraffin-NBP1-81615-img0002.jpg)
![Western Blot: MCRS1 Antibody [NBP1-81615] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/MCRS1-Antibody-Western-Blot-NBP1-81615-img0005.jpg)
![Western Blot: NCAPH Antibody [NBP1-32573] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Western-Blot-NBP1-32573-img0005.jpg "Western Blot: NCAPH Antibody [NBP1-32573] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-32573] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32573-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-32573] - Human gastric cancer, using BRRN1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32573-img0004.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Western blot analysis of extracts of various cell lines, using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Western-Blot-NBP3-16792-img0003.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Western blot analysis of extracts of various cell lines, using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Immunofluorescence analysis of U-2 OS cells using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Immunocytochemistry-Immunofluorescence-NBP3-16792-img0002.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Immunohistochemistry of paraffin-embedded human esophageal using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Immunohistochemistry-Paraffin-NBP3-16792-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Confocal imaging of paraffin-embedded Human colon cancer tissue using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16792_rabbit-ubiquitin-activating-enzyme-ube1-mab-6h2i9-5220251255534.jpg)
![Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.")