| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72427 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49174-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76843-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56310SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56369SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49354-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB33541-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22127-50ul | |||
![Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0002.jpg "Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ANP32B Antibody [NB100-1199] - LPS/IFN gamma-stimulated neuron-glial co-cultures (Glia/PAL31+ neuron) showing expression of both iNOS and PAL31. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1199-img0006.jpg)
![Immunohistochemistry: ANP32B Antibody [NB100-1199] - (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) and 1000X (E&F). Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-NB100-1199-img0005.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - (0.1 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-1199-img0001.jpg)
![Immunohistochemistry-Paraffin: ANP32B Antibody [NB100-1199] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Immunohistochemistry-Paraffin-NB100-1199-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ANP32B Antibody [NB100-1199] - Western blot analysis of pal31 siRNA-treated C6 showing knockdown of PAL31 expression by PAL31 silencer using 41.5 (lane1), 83 (lane2), and 166 (lane 3) picomole of pal31 siRNA or 332 picomole negative control. Actin works as a loading control. Image collected and cropped by CiteAb from the following publication (jbiomedsci.com/content/21/1/60), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Knockdown-Validated-NB100-1199-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Upregulated PAL31 expression was observed not only in microglia/ macrophage but also in glial cells near the injury site. (A) Normal thoracic spinal cord section showing few ED-1 positive cells (red). (B) Normal thoracic spinal cord section showing GFAP positive cells (red). (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing colocalization of upregulated PAL31 with astroglia (GFAP positive cells). (E) High power image showing the colocalization of upregulated PAL31 with ED1 positive macrophage/microglia (arrowhead) (F) High power image demonstrating the colocalization of upregulated PAL31 with GFAP positive astroglia (arrow). Magnification: 200X (A-D) & 1000X (E&F). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-3102024168397.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - Overexpression of PAL31 in C6 did not affect the cell proliferative activity. (A) GFP/bright field-superimposed micrograph in GFP overexpressing cells showing > 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-310202415525215.jpg)
![Western Blot: ANP32B Antibody [NB100-1199] - Effect of overexpression or knockdown of PAL31 in C6 on H2O2-induced toxicity. (A) Representative micrographs showing C6 overexpressing GFP or GFP-tagged PAL31 after being treated with H2O2 (0 ~ 1 mM) for 4 hours. (B) MTT assay in GFP- or GFP-PAL31-overexpressed C6 after H2O2 treatment showing significant difference (cytoprotective effect of PAL31) at 0.5 mM & 1 mM H2O2 treatment between C6/GFP & C6/PAL31 groups. The data in each dosage were analyzed by two-way ANOVA & Bonferroni post hoc test. *P 50% transfective efficiency (B) GFP-PAL31/bright field-micrograph in PAL31 overexpressing C6 showing correct nuclear localization of GFP-tagged PAL31 & transfection effeciency. (C) Western blot analysis showing the expression of GFP (~27 kDa), GFP-tagged PAL31 (~58 kDa), PCNA (~34 kDa), endogenous PAL31 (~31 kDa) & Actin (~43 kDa) in GFP- or PAL31-C6 cultures. The level of PCNA, a protein expressing in nuclear during DNA synthesis, in cells did not alter after overexpression of GFP or GFP-tagged PAL31. Actin works as a loading control. (D) MTT assay in the transfected cells at 4 different time intervals (from Ctrl to 72 hours). Ctrl represents the cells after subculture & overnight incubation. The data in each time points were analyzed by two-way ANOVA & Bonferroni post hoc test. No significance, compared GFP & GFP tagged PAL31 groups. n = 3. Magnification 100X (A-B). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25034417), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1199_goat-polyclonal-anp32b-antibody-31020241682198.jpg)
![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg)
![Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199] - Western blot analysis of extracts of various cell lines, using ANP32B Rabbit mAb (NBP3-16199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/ANP32B-Antibody-4A8E5-Western-Blot-NBP3-16199-img0006.jpg "Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199] - Western blot analysis of extracts of various cell lines, using ANP32B Rabbit mAb (NBP3-16199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded rat liver tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-72202512432045.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199] - Confocal imaging of C6 cells using ANP32B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-7220251243537.jpg)
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded rat lung tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-722025124534.jpg)
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded human liver tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-72202512435376.jpg)
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded human thyroid tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-7220251244334.jpg)
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded human esophagus tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-72202512432092.jpg)
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded mouse liver tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-72202512443396.jpg)
![Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199] - Confocal imaging of paraffin-embedded Mouse colon tissue using ANP32B Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-72202512443380.jpg)
![Immunohistochemistry: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry analysis of ANP32B in paraffin-embedded mouse heart tissue using ANP32B Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16199_rabbit-anp32b-mab-4a8e5-7220251244333.jpg)
![Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Western-Blot-NBP2-45549-img0015.jpg "Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.")
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0014.jpg)
![Flow Cytometry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Flow-Cytometry-NBP2-45549-img0001.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0002.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0003.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0004.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0005.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0006.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0007.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0008.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0009.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0010.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0011.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0012.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0013.jpg)
![Western Blot: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Western-Blot-NBP2-72427-img0001.jpg "Western Blot: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.")
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0014.jpg)
![Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of live Sup-B15 cells, using BTLA antibody , compared to IgG1 isotype control (Black).](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72427-img0002.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0003.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0004.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0005.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0006.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0007.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0008.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0009.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0010.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0011.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0012.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0013.jpg)
![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) - Azide and BSA Free [NBP2-72427] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-OTI2E4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72427-img0015.jpg)
![Western Blot: TCFL5 Antibody [NBP2-49174] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Western-Blot-NBP2-49174-img0006.jpg "Western Blot: TCFL5 Antibody [NBP2-49174] - Analysis in human cell line MOLT-4.")
![Immunocytochemistry/Immunofluorescence: TCFL5 Antibody [NBP2-49174] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49174-img0004.jpg)
![Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunohistochemistry-Paraffin-NBP2-49174-img0012.jpg)
![Western Blot: TCFL5 Antibody [NBP2-49174] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/TCFL5-Antibody-Western-Blot-NBP2-49174-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Analysis in human testis and pancreas tissues. Corresponding TCFL5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunohistochemistry-Paraffin-NBP2-49174-img0007.jpg)
![Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunohistochemistry-Paraffin-NBP2-49174-img0008.jpg)
![Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Staining of human rectum shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunohistochemistry-Paraffin-NBP2-49174-img0009.jpg)
![Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunohistochemistry-Paraffin-NBP2-49174-img0010.jpg)
![Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TCFL5-Antibody-Immunohistochemistry-Paraffin-NBP2-49174-img0011.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF/BLyS/TNFSF13B antibody at 1 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-271120242010580.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 ug of lysates per lane.Antibodies: BAFF/BLyS/TNFSF13B (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285892.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF/BLyS/TNFSF13B with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285848.gif)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NB100-56310-img0003.jpg "Western Blot: BAFF/BLyS/TNFSF13B Antibody [NB100-56310] - Analysis of BAFF/BLyS/TNFSF13B in mouse and human lysates using BLyS antibody at 2 ug/ml. Lane A: mouse testis, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human). Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0001.jpg "Western Blot: ANP32B Antibody [NB100-56369] - Western blot analysis of APRIL in HL-60 whole cell lysate using NB100-56369 at 2 ug/ml.")
![Western Blot: ANP32B Antibody [NB100-56369] - analysis of APRIL in HL-60 whole cell lysate using APRIL antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/ANP32B-Antibody-Western-Blot-NB100-56369-img0002.jpg)
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human tonsil shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0009.jpg "Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human tonsil shows moderate positivity in lymphoid cells.")
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0006.jpg)
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0007.jpg)
![Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining of human small intestine shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BTLA/CD272 Antibody [NBP2-49354] - Staining in human tonsil and pancreas tissues . Corresponding BTLA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BTLA-CD272-Antibody-Immunohistochemistry-Paraffin-NBP2-49354-img0010.jpg)
![Western Blot: ANP32B Antibody (SR1488) [NBP3-22127] - Western blot analysis using NBP3-22127 in (1) Jurkat cell lysate; (2) RAW264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22127_rabbit-anp32b-mab-sr1488-57202316582132.jpg "Western Blot: ANP32B Antibody (SR1488) [NBP3-22127] - Western blot analysis using NBP3-22127 in (1) Jurkat cell lysate; (2) RAW264.7 cell lysate.")