| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47836-0.1mg | |||
| IHC-Prediluted | NBP2-48321 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33321-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23891SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56339SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47837-0.1mg | |||
| IHC-Prediluted | NBP2-48322 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27173SS | |||
![Western Blot: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Western Blot Analysis of PC3 cell lysate usingOrnithine Decarboxylase antibody (ODC1/486).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Western-Blot-NBP2-44734-img0005.jpg "Western Blot: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Western Blot Analysis of PC3 cell lysate usingOrnithine Decarboxylase antibody (ODC1/486).")
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Ornithine Decarboxylase Antibody (ODC1/486).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0004.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Human Pancreas stained with ODC1 Monoclonal Antibody (ODC1/486)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0001.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Rat Pancreas stained with ODC1 Monoclonal Antibody (ODC1/486)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0002.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/486) [NBP2-44734] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/486).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-486-Immunohistochemistry-Paraffin-NBP2-44734-img0003.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Western-Blot-NBP2-33320-img0017.jpg "Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Immunohistochemistry-Paraffin-NBP2-33320-img0015.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Flow-Cytometry-NBP2-33320-img0018.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0007.jpg)
![Simple Western: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Simple-Western-NBP2-33320-img0009.jpg)
![Protein Array: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ER alpha/NR3A1 Antibody (ER506). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-ER506-Protein-Array-NBP2-33320-img0016.jpg)
![Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - Gastric cancer. RRM1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RRM1-Antibody-Immunohistochemistry-Paraffin-NBP2-20231-img0005.jpg "Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - Gastric cancer. RRM1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: RRM1 Antibody [NBP2-20231] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug Raw264. 7 whole cell lysate/extract C. 30 ug C2C12 whole cell lysate/extract5 % SDS-PAGE RRM1 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/RRM1-Antibody-Western-Blot-NBP2-20231-img0001.jpg)
![Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - Human colon carcinoma, using RRM1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RRM1-Antibody-Immunohistochemistry-Paraffin-NBP2-20231-img0003.jpg)
![Western Blot: RRM1 Antibody [NBP2-20231] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody (NBP2-20231) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20231_rabbit-polyclonal-rrm1-antibody-91020242011491.jpg)
![Immunocytochemistry/ Immunofluorescence: RRM1 Antibody [NBP2-20231] - RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: RRM1 stained by RRM1 antibody (NBP2-20231) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20231_rabbit-polyclonal-rrm1-antibody-9102024205653.jpg)
![Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - RRM1 antibody detects RRM1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.RRM1 stained by RRM1 antibody (NBP2-20231) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20231_rabbit-polyclonal-rrm1-antibody-910202420122048.jpg)
![Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP2-36737-img0001.jpg "Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP2-36737] - Analysis of FFPE section of human testis using Aurora A antibody at 10 ug/ml concentration. Heat-induced antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP2-36737-img0002.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-23891-img0002.jpg "Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.")
![Flow Cytometry: Dicer Antibody [NBP2-23891] - Analysis of HeLa cells transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using this antibody. Purple represents isotype control staining.](http://images.novusbio.com/fullsize/Dicer-Antibody-Flow-Cytometry-NBP2-23891-img0003.jpg)
![ELISA: Dicer Antibody [NBP2-23891] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.](http://images.novusbio.com/fullsize/Dicer-Antibody-ELISA-NBP2-23891-img0001.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Western-Blot-NBP2-24679-img0002.jpg "Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.")
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)
![Western Blot: Mre11 Antibody [NB100-56339] - Western blot analysis of 293 lysate blotted with MRE11 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Mre11-Antibody-Western-Blot-NB100-56339-img0003.jpg "Western Blot: Mre11 Antibody [NB100-56339] - Western blot analysis of 293 lysate blotted with MRE11 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: Mre11 Antibody [NB100-56339] - Paraffin-embedded human testis using MRE11 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-Paraffin-NB100-56339-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunocytochemistry-Immunofluorescence-NBP2-44735-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).")
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Ornithine Decarboxylase Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0007.jpg)
![Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - An intracellular stain was performed on K562 cells with Ornithine Decarboxylase [ODC1/487] Antibody NBP2-47837AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Flow-Cytometry-NBP2-44735-img0004.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Rat Pancreas stained with ODC1 Monoclonal Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0001.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/487)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-487-Immunohistochemistry-Paraffin-NBP2-44735-img0006.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Western Blot: GFI-1 Antibody [NBP2-27173] - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/GFI-1-Antibody-Western-Blot-NBP2-27173-img0004.jpg "Western Blot: GFI-1 Antibody [NBP2-27173] - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173] - Analysis of Gfi-1 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/GFI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27173-img0003.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0012.jpg "Western Blot: CHAF1A Antibody [NB100-74608] - RECQ5 promotes SUMO2 conjugation of PCNA. Western blot analysis of the indicated proteins in WCE prepared from control or RECQ5 siRNA knockdown HEK293T cells with exogenous overexpression of indicated FLAG-tagged PCNA constructs or CAF1 (CHAF1A) or treated with an empty vector (V). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05236-y), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74608] - Human colon carcinoma peyer's patch. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunohistochemistry-Paraffin-NB100-74608-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CHAF1A Antibody [NB100-74608] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74608. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74609 and NB100-74610.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74608-img0011.jpg)
![Western Blot: CHAF1A Antibody [NB100-74608] - SUMO2-PCNA enriches CAF1 & FACT within the replication complex. a Western blot analysis of the indicated proteins in the CB fractions (left) & the FLAG-PCNA complexes purified from CB fractions (right) prepared from cells expressing the indicated FLAG-tagged proteins. Ub-KR indicates the FLAG-ubiquitin-PCNA (KR) fusion protein. b Western blot analysis of the indicated proteins in the CB input (left panels) used for the in vitro pull-down experiments of purified recombinant strep-tagged PCNA, SUMO2-conjugated PCNA, & GFP anchored to Strep-Tactin resins (right panels). SUMO2-modified PCNA was generated by the in vitro SUMOylation reaction using strep-PCNA & His-SUMO2 shown in Supplementary Fig. 6a, b. c Western blot analysis of the input levels of recombinant strep-PCNA & strep-SUMO2-PCNA fusion (S2-PCNA) purified from E. coli (left panel) & of strep-PCNA & strep-S2-PCNA pulled down by WT or SIM mutant FLAG-CAF1A proteins purified & anchored on FLAG M2 agarose beads from HEK293T cells expressing the corresponding constructs (right panels). d Western blot analysis of strep-S2-PCNA (upper 2 panels) & strep-PCNA (lower 2 panels) pulled down by FLAG-CAF1A purified from E. coli & anchored on FLAG M2 agarose beads. e Western blot analysis of the indicated proteins in the CB input prepared from HEK293T cells expressing FLAG-SSRP1 WT or SIM mutant (left panels) & of FLAG-SSRP1 & SPT16 pulled down from the CB fractions in vitro using purified recombinant strep-tagged PCNA, S2-PCNA, & GFP anchored to Strep-Tactin resins (right panels) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30006506), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74608_rabbit-polyclonal-chaf1a-antibody-310202416575.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB100-418-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunoprecipitation-NB100-418-img0006.jpg)