| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Cy3 | NB120-11319 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12632-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00051-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12579-50ul | |||
![Western Blot: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY t-Plasminogen.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Western-Blot-NBP2-74568-img0001.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY t-Plasminogen.")
![Immunohistochemistry: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74568-img0006.jpg)
![Immunohistochemistry: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74568-img0002.jpg)
![Immunohistochemistry: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74568-img0003.jpg)
![Immunohistochemistry: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74568-img0004.jpg)
![Immunohistochemistry: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74568-img0005.jpg)
![Immunohistochemistry: t-Plasminogen Activator/tPA Antibody (OTI3H3) - Azide and BSA Free [NBP2-74568] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74568-img0007.jpg)
![Western Blot: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARPC5.](http://images.novusbio.com/fullsize/ARPC5-Antibody-OTI2G1-Azide-and-BSA-Free-Western-Blot-NBP2-71550-img0002.jpg "Western Blot: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARPC5.")
![Immunohistochemistry: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ARPC5-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71550-img0005.jpg)
![Western Blot: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of extracts (10ug) from 4 different cell lines.](http://images.novusbio.com/fullsize/ARPC5-Antibody-OTI2G1-Azide-and-BSA-Free-Western-Blot-NBP2-71550-img0001.jpg)
![Immunohistochemistry: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ARPC5-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71550-img0003.jpg)
![Immunohistochemistry: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ARPC5-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71550-img0004.jpg)
![Immunohistochemistry: ARPC5 Antibody (OTI2G1) - Azide and BSA Free [NBP2-71550] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ARPC5-Antibody-OTI2G1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71550-img0006.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Western-Blot-NBP2-70688-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0006.jpg)
![Western Blot: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.](http://images.novusbio.com/fullsize/HAND1-Antibody-OTI1G10-Azide-and-BSA-Free-Western-Blot-NBP2-71459-img0002.jpg "Western Blot: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.")
![Immunocytochemistry/Immunofluorescence: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.](http://images.novusbio.com/fullsize/HAND1-Antibody-OTI1G10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71459-img0005.jpg)
![Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/HAND1-Antibody-OTI1G10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71459-img0004.jpg)
![Western Blot: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/HAND1-Antibody-OTI1G10-Azide-and-BSA-Free-Western-Blot-NBP2-71459-img0001.jpg)
![Flow Cytometry: HAND1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-71459] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/HAND1-Antibody-OTI1G10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71459-img0003.jpg)
![Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Western-Blot-NB120-11318-img0015.jpg "Western Blot: gamma Tubulin Antibody [NB120-11318] - Cell lysates (lanes 1-7) were separated on SDS-PAGE, blotted with 0.7 ug/mL Anti-gamma-Tubulin, and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. A431")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in Rat2 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in NIH3T3 cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody [NB120-11318] - Centrosomal localization of gamma-tubulin in HeLa cells. Fixed cells were stained with 0.8 ug/mL Anti-gamma-Tubulin, followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (x100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11318-img0013.jpg)
![Western Blot: TUG Antibody [NBP3-12632] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:72 kDa](http://images.novusbio.com/fullsize/TUG-Antibody-Western-Blot-NBP3-12632-img0001.jpg "Western Blot: TUG Antibody [NBP3-12632] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:72 kDa")
![Immunohistochemistry-Paraffin: TUG Antibody [NBP3-12632] - Staining of human ASPSCR1 in human liver with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/TUG-Antibody-Immunohistochemistry-Paraffin-NBP3-12632-img0003.jpg)
![Immunohistochemistry-Paraffin: TUG Antibody [NBP3-12632] - Staining of human ASPSCR1 in human kidney with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/TUG-Antibody-Immunohistochemistry-Paraffin-NBP3-12632-img0002.jpg)
![Immunoprecipitation: TUG Antibody [NBP3-12632] - Lane A:0.5 mg K562 Whole Cell Lysate4 uL anti-ASPSCR1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ASPSCR1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size :72 kDa](http://images.novusbio.com/fullsize/TUG-Antibody-Immunoprecipitation-NBP3-12632-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TUG Antibody [NBP3-12632] - Staining of ASPSCR1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12632 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus?Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp3-12632_rabbit-polyclonal-tug-antibody-235202511254414.png)
![Western Blot: RAIDD/CRADD Antibody [NBP3-00051] - Anti-RAIDD/CRADD rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/RAIDD-CRADD-Antibody-Western-Blot-NBP3-00051-img0007.jpg "Western Blot: RAIDD/CRADD Antibody [NBP3-00051] - Anti-RAIDD/CRADD rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: RAIDD/CRADD Antibody [NBP3-00051] - Immunochemical staining of human RAIDD/CRADD in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/RAIDD-CRADD-Antibody-Immunohistochemistry-Paraffin-NBP3-00051-img0005.jpg)
![Immunohistochemistry-Paraffin: RAIDD/CRADD Antibody [NBP3-00051] - Immunochemical staining of human RAIDD/CRADD in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/RAIDD-CRADD-Antibody-Immunohistochemistry-Paraffin-NBP3-00051-img0004.jpg)
![Immunoprecipitation: RAIDD/CRADD Antibody [NBP3-00051] - RAIDD/CRADD was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 1 ul anti-RAIDD/CRADD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-RAIDD/CRADD rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa](http://images.novusbio.com/fullsize/RAIDD-CRADD-Antibody-Immunoprecipitation-NBP3-00051-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RAIDD/CRADD Antibody [NBP3-00051] - Immunofluorescence staining of RAIDD/CRADD in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-00051 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp3-00051_rabbit-polyclonal-raidd-cradd-antibody-235202511354128.png)
![Western Blot: PTRH2 Antibody [NBP3-12579] - Lane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PTRH2-Antibody-Western-Blot-NBP3-12579-img0001.jpg "Western Blot: PTRH2 Antibody [NBP3-12579] - Lane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP3-12579] - Staining of human PTRH2 in human liver with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PTRH2-Antibody-Immunohistochemistry-Paraffin-NBP3-12579-img0003.jpg)
![Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP3-12579] - Staining of human PTRH2 in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PTRH2-Antibody-Immunohistochemistry-Paraffin-NBP3-12579-img0002.jpg)
![Immunoprecipitation: PTRH2 Antibody [NBP3-12579] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PTRH2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PTRH2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size :19 kDa](http://images.novusbio.com/fullsize/PTRH2-Antibody-Immunoprecipitation-NBP3-12579-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PTRH2 Antibody [NBP3-12579] - Staining of PTRH2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12579 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12579_rabbit-polyclonal-ptrh2-antibody-235202514371335.png)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Western Blot: Ninein Antibody (PSH03-46) [NBP3-32652] - Western blot analysis of Ninein on Ninein recombinant protein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. Lysates/proteins at 20 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32652) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-278202416285254.jpg "Western Blot: Ninein Antibody (PSH03-46) [NBP3-32652] - Western blot analysis of Ninein on Ninein recombinant protein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. Lysates/proteins at 20 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32652) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Ninein Antibody (PSH03-46) [NBP3-32652] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Ninein antibody (NBP3-32652) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32652) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-298202414161899.jpg)
![Immunocytochemistry/ Immunofluorescence: Ninein Antibody (PSH03-46) [NBP3-32652] - Immunocytochemistry analysis of NIH/3T3 cells labeling Ninein with Rabbit anti-Ninein antibody (NBP3-32652) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ninein antibody (NBP3-32652) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-29202410334027.jpg)
![Flow Cytometry: Ninein Antibody (PSH03-46) [NBP3-32652] - Flow cytometric analysis of NIH/3T3 cells labeling Ninein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32652, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32652_rabbit-ninein-mab-psh03-46-29202416372136.jpg)
![Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-278202416285274.jpg "Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD38 Antibody (PD01-49) [NBP3-32151] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD38 antibody (NBP3-32151) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32151) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29820241435231.jpg)
![Immunocytochemistry/ Immunofluorescence: CD38 Antibody (PD01-49) [NBP3-32151] - Immunocytochemistry analysis of A549 cells labeling CD38 with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-2920241192330.jpg)
![Flow Cytometry: CD38 Antibody (PD01-49) [NBP3-32151] - Flow cytometric analysis of THP-1 cells labeling CD38. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32151, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29202416453252.jpg)
![Western Blot: NSUN3 Antibody (PSH01-97) [NBP3-32665] - Western blot analysis of NSUN3 on different lysates with Rabbit anti-NSUN3 antibody (NBP3-32665) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: K-562 cell lysate (40 ug/Lane) Lane 3: HL-60 cell lysate (40 ug/Lane) Lane 4: 293T cell lysate (40 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: Mouse colon tissue lysate (40 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32665) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32665_rabbit-nsun3-mab-psh01-97-27820241628524.jpg "Western Blot: NSUN3 Antibody (PSH01-97) [NBP3-32665] - Western blot analysis of NSUN3 on different lysates with Rabbit anti-NSUN3 antibody (NBP3-32665) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: K-562 cell lysate (40 ug/Lane) Lane 3: HL-60 cell lysate (40 ug/Lane) Lane 4: 293T cell lysate (40 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: Mouse colon tissue lysate (40 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32665) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NSUN3 Antibody (PSH01-97) [NBP3-32665] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NSUN3 antibody (NBP3-32665) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32665) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32665_rabbit-nsun3-mab-psh01-97-29820241430415.jpg)
![Immunocytochemistry/ Immunofluorescence: NSUN3 Antibody (PSH01-97) [NBP3-32665] - Immunocytochemistry analysis of K-562 cells labeling NSUN3 with Rabbit anti-NSUN3 antibody (NBP3-32665) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN3 antibody (NBP3-32665) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32665_rabbit-nsun3-mab-psh01-97-2920241033406.jpg)
![Flow Cytometry: NSUN3 Antibody (PSH01-97) [NBP3-32665] - Flow cytometric analysis of K-562 cells labeling NSUN3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32665, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32665_rabbit-nsun3-mab-psh01-97-29202416453241.jpg)