| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49678SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-91761SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24389SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25093-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0002.jpg "Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Simple Western: PINK1 Antibody [NBP1-49678] - Lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~61kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NBP1-49678-img0006.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NBP1-49678] - Stain in paraffin embedded mouse brain.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NBP1-49678-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested at 1:50 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0005.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0018.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0015.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0016.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0017.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining in human skin and pancreas tissues using anti-POSTN antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82472-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining in human skin and pancreas tissues using anti-POSTN antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Western-Blot-NBP1-82472-img0005.jpg)
![Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82472-img0006.jpg)
![Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82472-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Characteristics of lung cancer cell lines according to Periostin/OSF-2 (POSTN) expression. Comparison of Periostin/OSF-2 expression levels detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/23/3/1240) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody-Knockdown-Validated-NBP1-82472-img0009.jpg)
![Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-82472] - Section of non-small cell lung carcinoma (NSCLC) immunostained for Periostin/OSF-2 in cancer-associated fibroblasts (CAFs). Magnification 50×. Bar = 200 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32987711/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-82472_rabbit-periostin-osf-2-pab-20920231742123.jpg)
![Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - The efficacy of shRNA lentiviral particles transfection. (A) Silencing of periostin (POSTN) expression was confirmed by Western blot analysis. beta -actin was used as an internal control. (B) Confocal images & fluorescence analysis showing the expression of POSTN in control A549.CTRL cells & A549.shRNA cells (*** p 0.05). Objective 60×/1.40 Oil; pinhole airy 1.25. Bar = 20 µm. (C) Immunohistochemical expression level of periostin (POSTN) in the tissue of particular non-small cell lung carcinoma (NSCLC) subtypes: squamous cell carcinoma (SCC) & adenocarcinoma (AC) (p > 0.05). IRS, immunoreactive score. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35163164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82472_rabbit-periostin-osf-2-pab-310202415392583.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg "Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).")
![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg)
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of human placenta tissue lysate using Factor VIII antibody at 1:500.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0002.jpg)
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 with ECL based detection.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0006.jpg)
![Western Blot: CCDC109A Antibody [NBP1-88534] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCU antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Western-Blot-NBP1-88534-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CCDC109A Antibody [NBP1-88534] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCU antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0014.jpg)
![Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0008.jpg)
![Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0009.jpg)
![Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human hippocampus shows moderate to strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-Paraffin-NBP1-88534-img0010.jpg)
![Simple Western: CCDC109A Antibody [NBP1-88534] - Simple Western lane view shows a specific band for MCU in 0.2 mg/ml of RT-4 (left), A549 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Simple-Western-NBP1-88534-img0005.jpg)
![Simple Western: CCDC109A Antibody [NBP1-88534] - Electropherogram image(s) of corresponding Simple Western lane view. CCDC109A antibody was used at 1:20 dilution on RT-4 and A549 lysate(s).](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Simple-Western-NBP1-88534-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)
![Western Blot: Kv1.5 Antibody [NBP2-76939] - Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Western-Blot-NBP2-76939-img0001.jpg "Western Blot: Kv1.5 Antibody [NBP2-76939] - Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1:5000 dilution.")
![Immunocytochemistry/Immunofluorescence: Kv1.5 Antibody [NBP2-76939] - ICC staining KCNA5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76939-img0004.jpg)
![Immunohistochemistry-Paraffin: Kv1.5 Antibody [NBP2-76939] - Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-76939-img0007.jpg)
![Flow Cytometry: Kv1.5 Antibody [NBP2-76939] - Flow cytometric analysis of PANC-1 cells with KCNA5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Flow-Cytometry-NBP2-76939-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Kv1.5 Antibody [NBP2-76939] - ICC staining KCNA5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76939-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Kv1.5 Antibody [NBP2-76939] - ICC staining KCNA5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76939-img0005.jpg)
![Immunohistochemistry-Paraffin: Kv1.5 Antibody [NBP2-76939] - Rodent model of heart failure post-myocardial infarction. 60X micrograph from infarct region. Antibody at 1:1000. Nuclear counterstain. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-76939-img0012.jpg)
![Immunohistochemistry-Paraffin: Kv1.5 Antibody [NBP2-76939] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Kv1.5-Antibody-Immunohistochemistry-Paraffin-NBP2-76939-img0006.jpg)
![Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-24389-img0001.jpg "Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-24389] - Immunohistochemistry analysis of human kidney using this antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-24389-img0002.jpg)
![Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)
![Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP3-11775-img0003.jpg "Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0008.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of mouse testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0002.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on Neuro2a cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0007.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0001.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on THP-1 cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0004.jpg)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-2711202419333416.gif "<b>Genetic Strategies Validation. </b>Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - KO Validation in 293T Cells Loading: 10 ug of lysate Antibodies: beta Amyloid, 0.5 ug/mL and beta-actin, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain tissue with beta Amyloid antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241918631.gif)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunohistochemistry of beta Amyloid in human brain (Alzheimer's disease) tissue with beta Amyloid antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242016992.jpg)
![Immunohistochemistry: beta Amyloid Antibody - BSA Free [NBP2-25093] - Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 ug/mL.Green: beta Amyloid Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120241939277.gif)
![Western Blot: beta Amyloid Antibody - BSA Free [NBP2-25093] - Western blot analysis of beta Amyloid in (A) human, (B) mouse, and (C) rat brain tissue lysates with beta Amyloid antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-25093_rabbit-polyclonal-beta-amyloid-antibody-271120242022942.jpg)