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![Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/SLC6A2-NET-Noradrenaline-transporter-Antibody-Western-Blot-NBP1-60120-img0008.jpg "Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Human Adult Placenta](http://images.novusbio.com/fullsize/SLC6A2-NET-Noradrenaline-transporter-Antibody-Western-Blot-NBP1-60120-img0005.jpg)
![Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Tissue:Human Fetal Lung.](http://images.novusbio.com/fullsize/SLC6A2-NET-Noradrenaline-transporter-Antibody-Western-Blot-NBP1-60120-img0006.jpg)
![Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Sample Tissue: Human Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/SLC6A2-NET-Noradrenaline-transporter-Antibody-Western-Blot-NBP1-60120-img0007.jpg)
![Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Titration: 0.2-1 ug/ml, Positive Control: Human heart.](http://images.novusbio.com/fullsize/SLC6A2-NET-Noradrenaline-transporter-Antibody-Western-Blot-NBP1-60120-img0003.jpg)
![Western Blot: SLC6A2/NET/Noradrenaline transporter Antibody [NBP1-60120] - Human Fetal Heart.](http://images.novusbio.com/fullsize/SLC6A2-NET-Noradrenaline-transporter-Antibody-Tissue-Culture-Substratum-NBP1-60120-img0004.jpg)
![Western Blot: HADHA Antibody (PSH01-28) [NBP3-32414] - Western blot analysis of HADHA on different lysates with Rabbit anti-HADHA antibody (NBP3-32414) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: COS-1 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Human kidney tissue lysate Lane 10: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 83 kDa Observed band size: 74 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32414) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32414_rabbit-hadha-mab-psh01-28-278202415242638.jpg "Western Blot: HADHA Antibody (PSH01-28) [NBP3-32414] - Western blot analysis of HADHA on different lysates with Rabbit anti-HADHA antibody (NBP3-32414) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: COS-1 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Human kidney tissue lysate Lane 10: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 83 kDa Observed band size: 74 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32414) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HADHA Antibody (PSH01-28) [NBP3-32414] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-HADHA antibody (NBP3-32414) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32414) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32414_rabbit-hadha-mab-psh01-28-298202413585542.jpg)
![Immunocytochemistry/ Immunofluorescence: HADHA Antibody (PSH01-28) [NBP3-32414] - Immunocytochemistry analysis of HeLa cells labeling HADHA with Rabbit anti-HADHA antibody (NBP3-32414) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HADHA antibody (NBP3-32414) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32414_rabbit-hadha-mab-psh01-28-29202410334054.jpg)
![Immunohistochemistry-Paraffin: MYL7 Antibody [NBP2-19449] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MYL7-Antibody-Immunohistochemistry-Paraffin-NBP2-19449-img0003.jpg "Immunohistochemistry-Paraffin: MYL7 Antibody [NBP2-19449] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.")
![Western Blot: MYL7 Antibody [NBP2-19449] - MYL7 antibody detects MYL7 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MYL7 antibody (NBP2-19449) diluted by 1:1000.](http://images.novusbio.com/fullsize/nbp2-19449_rabbit-polyclonal-myl7-antibody-91020242024620.jpg)
![Immunocytochemistry/Immunofluorescence: CYP2R1 Antibody [NBP1-32883] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CYP2R1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32883-img0005.jpg "Immunocytochemistry/Immunofluorescence: CYP2R1 Antibody [NBP1-32883] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CYP2R1 Antibody [NBP1-32883] - Mouse liver. Cytochrome P450 2R1 antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CYP2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-32883-img0007.jpg)
![Immunohistochemistry-Paraffin: CYP2R1 Antibody [NBP1-32883] - FaDu xenograft, using CYP2R1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CYP2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-32883-img0003.jpg)
![Immunohistochemistry-Paraffin: CYP2R1 Antibody [NBP1-32883] - Rat brain. Cytochrome P450 2R1 antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CYP2R1-Antibody-Immunohistochemistry-Paraffin-NBP1-32883-img0006.jpg)
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0010.jpg "Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane.Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C.Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.(Right Blot-red)Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT.Block overnight at 4C.Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.")
![Western Blot: Lysozyme Antibody [NBP1-77790] - Western Blot of Lysozyme antibody.Lane 1: Lysozyme (Hen Egg White).Load: 50 ng per lane.Primary antibody: Lysozyme antibody at 1:1,000 overnight at 4C.Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).](http://images.novusbio.com/fullsize/Lysozyme-Antibody-Western-Blot-NBP1-77790-img0009.jpg)
![ELISA: Lysozyme Antibody [NBP1-77790] - ELISA Results of Lysozyme antibody. Each well was coated in duplicate with 1.0 ug of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-ELISA-NBP1-77790-img0011.jpg)
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (NBP2-43557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43557_rabbit-polyclonal-hemoglobin-epsilon-antibody-91020242041254.jpg "Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (NBP2-43557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (NBP2-43557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43557_rabbit-polyclonal-hemoglobin-epsilon-antibody-91020241959350.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-43557_rabbit-polyclonal-hemoglobin-epsilon-antibody-1610202419405766.jpg)
![Untreated (-) and treated (+) mouse tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (NBP2-43557) diluted at 1:500. (CFA: Complete Freunds adjuvant)](http://images.novusbio.com/fullsize/nbp2-43557_rabbit-polyclonal-hemoglobin-epsilon-antibody-western-blot-2611202518475724.jpg)
![Western Blot: FSTL1 Antibody [NBP2-16539] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FSTL1-Antibody-Western-Blot-NBP2-16539-img0001.jpg "Western Blot: FSTL1 Antibody [NBP2-16539] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: FSTL1 Antibody [NBP2-16539] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FSTL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16539-img0003.jpg)
![Immunohistochemistry-Paraffin: FSTL1 Antibody [NBP2-16539] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/FSTL1-Antibody-Immunohistochemistry-Paraffin-NBP2-16539-img0002.jpg)
![U87-MG whole cell extract and conditioned medium (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FSTL1 antibody [C2C3], C-term (NBP2-16539) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16539_rabbit-polyclonal-follistatin-like-1-fstl1-antibody-western-blot-261120251840487.jpg)
![Western Blot: NAB1 Antibody [NBP1-71838] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NAB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB1 was also immunoprecipitated by rabbit anti-NAB1 antibody NBP1-71837, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NAB1-Antibody-Western-Blot-NBP1-71838-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NAB1 Antibody [NBP1-71838] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NAB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB1 was also immunoprecipitated by rabbit anti-NAB1 antibody NBP1-71837, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: NAB1 Antibody [NBP1-71838] - Simple Western lane view shows a specific band for NAB1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NAB1-Antibody-Simple-Western-NBP1-71838-img0002.jpg)
![Western Blot: MCTS1 Antibody [NBP2-17242] - Non-transfected (-) and transfected (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MCTS1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MCTS1-Antibody-Western-Blot-NBP2-17242-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MCTS1 Antibody [NBP2-17242] - Non-transfected (-) and transfected (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MCTS1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: MCTS1 Antibody [NBP2-17242] - Rat spleen. MCTS1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MCTS1-Antibody-Immunohistochemistry-Paraffin-NBP2-17242-img0005.jpg)
![Western Blot: MCT1 Antibody [NBP2-17242] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MCT1-Antibody-Western-Blot-NBP2-17242-img0001.jpg)
![Immunohistochemistry-Paraffin: MCTS1 Antibody [NBP2-17242] - Mouse lung. MCTS1 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MCTS1-Antibody-Immunohistochemistry-Paraffin-NBP2-17242-img0004.jpg)
![Western Blot: RHCG Antibody (5A4) [H00051458-M06] - Analysis of RHCG expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/RHCG-Antibody-5A4-Western-Blot-H00051458-M06-img0004.jpg "Western Blot: RHCG Antibody (5A4) [H00051458-M06] - Analysis of RHCG expression in PC-12 (Cat # L012V1).")
![Immunohistochemistry-Paraffin: RHCG Antibody (5A4) [H00051458-M06] - Analysis of monoclonal antibody to RHCG on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/RHCG-Antibody-5A4-Immunohistochemistry-Paraffin-H00051458-M06-img0003.jpg)
![DHCR7 antibody [HL3854] detects DHCR7 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.DHCR7 stained by DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48851_rabbit-dhcr7-mab-hl3854-immunohistochemistry-paraffin-141120251829410.jpg "<b>Orthogonal Strategies Validation. </b>DHCR7 antibody [HL3854] detects DHCR7 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.DHCR7 stained by DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48851_rabbit-dhcr7-mab-hl3854-western-blot-1411202518432421.jpg)
![Boiled and unboiled MCF-7 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp3-48851_rabbit-dhcr7-mab-hl3854-western-blot-1411202518394117.jpg)
![DHCR7 antibody [HL3854] detects DHCR7 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.DHCR7 stained by DHCR7 antibody [HL3854] (NBP3-48851) diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48851_rabbit-dhcr7-mab-hl3854-immunohistochemistry-paraffin-1411202518412913.jpg)