| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36430-0.025mg | |||
| Azide and BSA Free | NBP2-80935 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47946-0.1mg | |||
| IHC-Prediluted | NBP2-48095 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54419-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52724SS | |||
| Azide and BSA Free | NBP2-80891 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52722SS | |||
| Azide and BSA Free | NBP2-80890 | |||
![Immunocytochemistry/Immunofluorescence: AF9 Antibody [NBP2-15303] - AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 protein stained by AF9 antibody diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15303-img0004.jpg "Immunocytochemistry/Immunofluorescence: AF9 Antibody [NBP2-15303] - AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 protein stained by AF9 antibody diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse liver. AF9 stained by AF9 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-Paraffin-NBP2-15303-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: AF9 Antibody [NBP2-15303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/AF9-Antibody-Western-Blot-NBP2-15303-img0006.jpg)
![Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - D54 xenograft, using AF9 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-Paraffin-NBP2-15303-img0002.jpg)
![Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse brain. AF9 stained by AF9 antibody diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AF9-Antibody-Immunohistochemistry-Paraffin-NBP2-15303-img0007.jpg)
![Western Blot: AF9 Antibody [NBP2-15303] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (NBP2-15303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15303_rabbit-polyclonal-af9-antibody-91020241957219.jpg)
![Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-27208-img0002.jpg "Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.")
![Western Blot: S100A4 Antibody (1F11) [NBP2-36430] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36430) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/S100A4-Antibody-1F11-Knockout-Validated-NBP2-36430-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: S100A4 Antibody (1F11) [NBP2-36430] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36430) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: S100A4 Antibody (1F11) [NBP2-36430] - Analysis of S100A4 (1F11) in A375 and A549 cell lysate.](http://images.novusbio.com/fullsize/S100A4-Antibody-1F11-Western-Blot-NBP2-36430-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1F11) [NBP2-36430] - S100A4 (1F11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/S100A4-Antibody-1F11-Immunocytochemistry-Immunofluorescence-NBP2-36430-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1F11) [NBP2-36430] - Analysis of FFPE tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, macrophages) developed nuclear staining also.](http://images.novusbio.com/fullsize/S100A4-Antibody-1F11-Immunohistochemistry-Paraffin-NBP2-36430-img0007.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1F11) [NBP2-36430] - Analysis of formalin-fixed paraffin-embedded tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/S100A4-Antibody-1F11-Immunohistochemistry-Paraffin-NBP2-36430-img0003.jpg)
![Immunohistochemistry-Paraffin: S100A4 Antibody (1F11) [NBP2-36430] - Analysis of FFPE tissue section of human normal skin with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml concentration. The epidermal cellular layers as well as the dermal connective tissue showed weak to negligible immunopositivity, however, very strong staining was observed in some dermal cells which are potentially the monocytes, blood vessels and the Langerhans cells.](http://images.novusbio.com/fullsize/S100A4-Antibody-1F11-Immunohistochemistry-Paraffin-NBP2-36430-img0006.jpg)
![Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Western-Blot-NBP2-44827-img0012.jpg "Western Blot: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Western Blot Analysis of human lung lysate using Cytokeratin 19 Antibody (KRT19/800).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19 using Cytokeratin 19 Antibody (KRT19/800) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunocytochemistry-Immunofluorescence-NBP2-44827-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Antibody (KRT19/800).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/800) [NBP2-44827] - Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44827-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody (NBP2-16842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16842_rabbit-polyclonal-hist1h3a-antibody-9102024202460.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)
![Western Blot: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Western Blot of human lung lysate using NBP2-53264.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Western-Blot-NBP2-53264-img0004.jpg "Western Blot: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Western Blot of human lung lysate using NBP2-53264.")
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Immunofluorescent staining of MCF-7 cells. NBP2-53264 labeled with CF488 (green). Nuclei are stained with Reddot (red)](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Immunocytochemistry-Immunofluorescence-NBP2-53264-img0007.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with EpCAM/TROP1 Antibody (EGP40/1372) - (ECD).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Immunohistochemistry-Paraffin-NBP2-53264-img0013.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM/TROP1 Antibody (EGP40/1372).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Flow-Cytometry-NBP2-53264-img0010.jpg)
![Western Blot: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Western Blot of 293, A431 and HCT116 Cell Lysate using EpCAM, Monoclonal Antibody (EGP40/1372).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Western-Blot-NBP2-53264-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Confocal Immunofluorescence of human Colorectal Carcinoma. NBP2-53264 labeled with CF488 (green); Nuclei are labeled with Reddot (red).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Immunocytochemistry-Immunofluorescence-NBP2-53264-img0006.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Formalin-fixed, paraffin-embedded Human Colorectal Carcinoma stained with EpCAM Monoclonal Antibody (EGP40/1372).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Immunohistochemistry-Paraffin-NBP2-53264-img0001.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with NBP2-53264.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Immunohistochemistry-Paraffin-NBP2-53264-img0003.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Formalin-fixed, paraffin-embedded canine (dog) bladder stained with EpCAM/TROP1 Antibody (EGP40/1372) - (ECD).](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Immunohistochemistry-Paraffin-NBP2-53264-img0012.jpg)
![Protein Array: EpCAM/TROP1 Antibody (EGP40/1372) - (ECD) [NBP2-53264] - Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM/TROP1 Antibody (EGP40/1372) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-EGP40-1372-ECD-Protein-Array-NBP2-53264-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) [NBP2-52724] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunocytochemistry-Immunofluorescence-NBP2-52724-img0002.jpg "Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) [NBP2-52724] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0006.jpg)
![Flow (Intracellular): OGG1 Antibody (2B4) [NBP2-52724] - An intracellular stain was performed on HeLa cells with OGG1 (2B4) antibody NBP2-52724 (Blue) and a matched isotype control NBP2-27231 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Flow-Intracellular-NBP2-52724-img0001.jpg)
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells rectum adenocarcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0004.jpg)
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells endometrial carcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0005.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)
![Immunocytochemistry/Immunofluorescence: OGG1 Antibody (4E8) [NBP2-52722] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (4E8) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OGG1-Antibody-4E8-Immunocytochemistry-Immunofluorescence-NBP2-52722-img0003.jpg "Immunocytochemistry/Immunofluorescence: OGG1 Antibody (4E8) [NBP2-52722] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (4E8) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: OGG1 Antibody (4E8) [NBP2-52722] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-52722-img0008.jpg)
![Flow (Intracellular): OGG1 Antibody (4E8) [NBP2-52722] - An intracellular stain was performed on HeLa cells with OGG1 (4E8) antibody NBP2-52722 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/OGG1-Antibody-4E8-Flow-Intracellular-NBP2-52722-img0002.jpg)
![Immunohistochemistry-Paraffin: OGG1 Antibody (4E8) [NBP2-52722] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells rectum adenocarcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-52722-img0006.jpg)
![Immunohistochemistry-Paraffin: OGG1 Antibody (4E8) [NBP2-52722] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells endometrial carcinoma.](http://images.novusbio.com/fullsize/OGG1-Antibody-4E8-Immunohistochemistry-Paraffin-NBP2-52722-img0007.jpg)
![Western Blot: DIS3 Antibody [H00022894-B01P] - analysis of DIS3 in HeLa whole cell extracts treated with (1) si-Control and (2) DIS3 siRNA using anti-DIS3 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/DIS3-Antibody-Western-Blot-H00022894-B01P-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DIS3 Antibody [H00022894-B01P] - analysis of DIS3 in HeLa whole cell extracts treated with (1) si-Control and (2) DIS3 siRNA using anti-DIS3 antibody. Image from verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: DIS3 Antibody [H00022894-B01P] - Analysis of DIS3 expression in transfected 293T cell line by DIS3 polyclonal antibody. Lane 1: KIAA1008 transfected lysate(105.38 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DIS3-Antibody-Western-Blot-H00022894-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DIS3 Antibody [H00022894-B01P] - Analysis of purified antibody to KIAA1008 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/DIS3-Antibody-Immunocytochemistry-Immunofluorescence-H00022894-B01P-img0003.jpg)