| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37291-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52979SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34921-1mg | |||
| Carrier-Free | NBP2-34921-100ug | |||
| Carrier-Free | NBP2-34921-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54426-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59619 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54559-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37349-0.025ml | |||
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0004.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Analysis of Hela cells with Interferon gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Flow-Cytometry-NBP2-66900-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Mouse liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver carcinoma tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0003.jpg)
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
![Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Dual-RNAscope-ISH-IHC-NBP2-37291-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Confocal image of hCD14-transfected HEKT cells, 4% formalin fixed, indirectly labeled with anti-CD14 1:200, and AF568-conjugated Ab 1:1000. Note that cells have different immunoreactivity due to various expression level of CD14 in single cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Immunocytochemistry-NBP2-37291-img0007.jpg)
![Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Flow-Cytometry-NBP2-37291-img0008.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0002.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0004.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0003.jpg)
![ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-ELISA-NBP2-37291-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody (NBP2-52979) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0006.jpg "Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody (NBP2-52979) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Western blot analysis using Apolipoprotein A-I/ApoA1 antibody. Harvested from mouse, separated on a 4-12 gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 3% BSA/TBST. Probed with 1:8000 anti-apoA1 in 1%BSA/TBST, and detect edwith an anti-rabbit HRP secondary antibody using chemiluminescence. WB dilution 1: 8000, block before incubation overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody conjugated to FITC (NBP2-52979F) at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0007.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse liver using APOA1 antibody at 1:400 dilution. The antibody generated a weak/mild cytoplasmic signal in hepatocytes with a very strong positivity near the hepatocyte membranes as well as in the inter-cellular spaces.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-Paraffin-NBP2-52979-img0002.jpg)
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-1/ApoA1 Antibody NBP2-52979 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Flow-Cytometry-NBP2-52979-img0004.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-APOA1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-APOA1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0001.jpg)
![SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]](http://images.novusbio.com/fullsize/Human-FGF-basic-FGF2-Protein-SDS-Page-NBP2-34921-img0001.jpg "SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]")
![Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Western-Blot-NBP2-53188-img0003.jpg "Western Blot: S100B Antibody (S100B/1706R) [NBP2-53188] - Western blot analysis of human brain tissue lysate using S100B antibody (S100B/1706R).")
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1706R) [NBP2-53188] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Rabbit Monoclonal Antibody (S100B/1706R).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Immunohistochemistry-Paraffin-NBP2-53188-img0001.jpg)
![Protein Array: S100B Antibody (S100B/1706R) [NBP2-53188] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100B Antibody (S100B/1706R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1706R-Protein-Array-NBP2-53188-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0007.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Protein Array: S100B Antibody (S100B/1012) [NBP2-59618] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Protein-Array-NBP2-59618-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (S100B/1012) [NBP2-59618] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-59618-img0006.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (S100B/1012) [NBP2-59618] - Formalin-fixed, paraffin-embedded human Schwanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).](http://images.novusbio.com/fullsize/S100B-Antibody-S100B-1012-Immunohistochemistry-Paraffin-NBP2-59618-img0004.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0010.jpg "Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0011.jpg)
![Flow Cytometry: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Flow-Cytometry-NBP2-67182-img0001.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-53187] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-53187-img0005.jpg "Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-53187] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Negative Control)")
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9 + S100B/1012) [NBP2-53187] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunohistochemistry-Paraffin-NBP2-53187-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9 + S100B/1012) [NBP2-53187] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Antibody (4C4.9 + S100B/1012) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-+-S100B-1012-Immunocytochemistry-Immunofluorescence-NBP2-53187-img0004.jpg)
![Western Blot: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Western blot analysis of extracts of various cell lines, using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CXCL4-PF4-Antibody-6Q10H2-Western-Blot-NBP3-16251-img0005.jpg "Western Blot: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Western blot analysis of extracts of various cell lines, using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Immunohistochemistry of paraffin-embedded rat lung using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CXCL4-PF4-Antibody-6Q10H2-Immunohistochemistry-Paraffin-NBP3-16251-img0001.jpg)
![Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Immunohistochemistry of paraffin-embedded mouse bone marrow using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CXCL4-PF4-Antibody-6Q10H2-Immunohistochemistry-Paraffin-NBP3-16251-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Confocal imaging of Mouse leg bone using CXCL4/PF4 Rabbit mAb. DAPI was used for nuclear staining (blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16251_rabbit-cxcl4-pf4-mab-6q10h2-52202511361215.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Western-Blot-NBP2-37349-img0004.jpg "Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of Hela cells using IL2RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Immunocytochemistry-Immunofluorescence-NBP2-37349-img0005.jpg)
![Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of bladder cancer tissues using IL2RA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Immunohistochemistry-NBP2-37349-img0007.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Western-Blot-NBP2-37349-img0002.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Western-Blot-NBP2-37349-img0003.jpg)
![Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of rectum cancer tissues using IL2RA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Immunohistochemistry-NBP2-37349-img0006.jpg)
![ELISA: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-ELISA-NBP2-37349-img0001.jpg)