| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33168 | |||
| IHC-Prediluted | NBP2-44590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37291-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81832-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89755-25ul | |||
![Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Western-Blot-NBP2-29403-img0001.jpg "Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunocytochemistry-Immunofluorescence-NBP2-29403-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - CODEX multiplexing image of S100b in primary melanoma of the skin. Image from verified customer review.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100B antibody(4C4.9).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0007.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Antibody (4C4.9)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0008.jpg)
![Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Dual-RNAscope-ISH-IHC-NBP2-37291-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Confocal image of hCD14-transfected HEKT cells, 4% formalin fixed, indirectly labeled with anti-CD14 1:200, and AF568-conjugated Ab 1:1000. Note that cells have different immunoreactivity due to various expression level of CD14 in single cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Immunocytochemistry-NBP2-37291-img0007.jpg)
![Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Flow-Cytometry-NBP2-37291-img0008.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0002.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - BMDM were unchallenged (control) or challenged with 500 HU/mL SLO WT, PFO or an equivalent mass of SLO ML for 10 min at 37C and centrifuged at 2000 x g for 5 min to yield cell pellet (C). Supernatants were spun at 100,000xg for 40 min at 4C to collect the high speed supernatant (S) and microvesicle pellet (M). Samples were solubilized at 95C in SDS-sample buffer, resolved by SDS-PAGE and transferred to nitrocellulose. Portions of the blot were probed with 6D11 anti-SLO, anti-IFNgammaR1, 4B4F12 anti-CD14, 76B357.1 anti-TLR4, O91B8 anti-MyD88, 1H4B01 anti-Trif, EPR4477 anti-Alkaline Phosphatase, MANLAC-4A7 anti-Lamin A/C, and AC-15 anti-beta-Actin, followed by relevant secondary antibodies and ECL. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24955-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0010.jpg)
![Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0003.jpg)
![ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD14-Antibody-4B4F12-ELISA-NBP2-37291-img0001.jpg)
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Western Blot: MIF Antibody [NBP1-81832] - Analysis in human cell lines HEK293 and U-251MG using Anti-MIF antibody. Corresponding MIF RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-81832-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: MIF Antibody [NBP1-81832] - Analysis in human cell lines HEK293 and U-251MG using Anti-MIF antibody. Corresponding MIF RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MIF Antibody [NBP1-81832] - Expression and purification of MaMIF. Western blot analysis with isotype-matched primary antibody shows the background level of antibody binding to the protein. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-51947-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-81832-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-81832] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81832-img0015.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0019.jpg)
![Western Blot: MIF Antibody [NBP1-81832] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-81832-img0011.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0016.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human kidney moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0017.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-81832] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-81832-img0018.jpg)
![Western Blot: MIF Antibody [NBP1-81832] - Expression & purification of MaMIF. (A) SDS-PAGE analysis of purified MaMIF suggesting an approximate molecular mass of ~12.5 kDa for the monomer. (B) Superdex 75 10/300 GL analytical size-exclusion chromatography profile of MaMIF suggesting an apparent molecular mass of ~39 kDa in solution, corresponding to the size of a trimeric form. (C) CD spectrum of MaMIF confirming a properly folded protein. The pie chart in the inset shows the secondary structure make-up of the protein. (D) Western blot analysis with polyclonal anti-His & anti-MIF antibodies confirming the expressed protein to be MaMIF. (E) Western blot analysis with isotype-matched primary antibody shows the background level of antibody binding to the protein. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31664114), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81832_rabbit-polyclonal-mif-antibody-31020241534334.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0004.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Analysis of Hela cells with Interferon gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Flow-Cytometry-NBP2-66900-img0001.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Mouse liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0002.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver carcinoma tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-JM10-10-Immunohistochemistry-Paraffin-NBP2-66900-img0003.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0010.jpg "Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0003.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0011.jpg)
![Flow Cytometry: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Flow-Cytometry-NBP2-67182-img0001.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0007.jpg)
![Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Human tonsil. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16948-img0006.jpg "Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Human tonsil. Image from verified customer review.")
![Flow Cytometry: IL-2 Antibody [NBP2-16948] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: IL2 antibody dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/IL-2-Antibody-Flow-Cytometry-NBP2-16948-img0007.jpg)
![Immunohistochemistry-Paraffin: IL-2 Antibody [NBP2-16948] - Human gastric cancer, using IL2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/IL-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16948-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GMF-beta Antibody [NBP1-89755] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GMF-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89755-img0015.jpg "Immunocytochemistry/Immunofluorescence: GMF-beta Antibody [NBP1-89755] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry: GMF-beta Antibody [NBP1-89755] - GMF-beta knockdown suppresses tumor growth & the formation of human-CD31 positive microvessels (hCD31-MVs) in orthotopic U87 xenograft modelsA. The cross-sectional appearance of xenografted gliomas from U87-mock group B. The cross-sectional appearance of xenografted gliomas from U87-shGMF-beta group. Gross tumor boundaries were delineated by white dotlines. C. A hCD31-microvessel & several hCD31-negative vessels in U87-mock tumor. D. No hCD31-MVs, only hCD31-negative vessels in U87-shGMF-beta tumor. Red solid arrows indicate hGMF-beta staining in tumor cells, black solid arrows show hCD31 staining on microvasular endothelia, & open arrows denote vessels negative for hCD31. Scale bar: 100 μm in upper pannels; 50 μm in lower pannels. E. Quantitative comparisons of gross tumor volume between U87-mock & U87-shGMF-beta group. F. Quantitative comparisons of hCD31-microvessel densities (MVDs) between U87-mock & U87-shGMF-beta group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26515590), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89755_rabbit-polyclonal-gmf-beta-antibody-310202416121977.jpg)
![Western Blot: GMF-beta Antibody [NBP1-89755] - Tubulogenesis of human U87 glioblastoma cells is inhibited by GMF-beta knockdownA. Protein levels of GMF-beta in human glial cell line (HEB) & human glioma cell lines of the different grades (CHG5, SHG44, U87). B. Assessment of GMF-beta knockdown in U87 cells by western blotting. C. Tube formation by U87 mock cells (left panel); impaired tube formation by U87-shGMF-beta cells (right panel). Inspection under a phase contrast fluorescent microscope (× 100). D. Quantified tubulogenesis of U87 mock cells & U87-shGMF-beta cells by pattern recognition system (left panel) & branch point counting system (right panel).*** indicates significant difference with P < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26515590), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89755_rabbit-polyclonal-gmf-beta-antibody-310202415371963.jpg)
![Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NB300-812-img0007.jpg "Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Human breast cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NB300-812-img0008.jpg)
![Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NB300-812-img0004.jpg)
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney. Antibody at 0.3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NB300-812-img0006.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Cross-linking analysis of fmHDL apolipoprotein. fmHDL (apo A-I), fmHDL (apo A-I-SH), and fmHDL (no apo A-I) were cross-linked with BS3. The apolipoprotein cross-link products were resolved on a 4-20% SDS PAGE gel and visualized by apo A-I immunoblotting. An oligomer ladder was prepared with lipid-free apo A-I (apo A-I lane) for cross-link product identification. fmHDL migrated on the PAGE gel as a clearly visible band and were detectible by apo A-I immunoblotting (marked as fmHDL on the left). Image collected and cropped by CiteAb from the following publication (//www.jlr.org/lookup/doi/10.1194/jlr.M054635) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NB400-147-img0004.jpg "Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Cross-linking analysis of fmHDL apolipoprotein. fmHDL (apo A-I), fmHDL (apo A-I-SH), and fmHDL (no apo A-I) were cross-linked with BS3. The apolipoprotein cross-link products were resolved on a 4-20% SDS PAGE gel and visualized by apo A-I immunoblotting. An oligomer ladder was prepared with lipid-free apo A-I (apo A-I lane) for cross-link product identification. fmHDL migrated on the PAGE gel as a clearly visible band and were detectible by apo A-I immunoblotting (marked as fmHDL on the left). Image collected and cropped by CiteAb from the following publication (//www.jlr.org/lookup/doi/10.1194/jlr.M054635) licensed under a CC-BY license.")
![Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-NB400-147-img0002.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Coomassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using anti-Human apolipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NB400-147-img0003.jpg)
