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![Western Blot: Rab24 Antibody (OTI3H1) - Azide and BSA Free [NBP2-73770] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB24.](http://images.novusbio.com/fullsize/Rab24-Antibody-OTI3H1-Azide-and-BSA-Free-Western-Blot-NBP2-73770-img0002.jpg "Western Blot: Rab24 Antibody (OTI3H1) - Azide and BSA Free [NBP2-73770] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB24.")
![Immunocytochemistry/Immunofluorescence: Rab24 Antibody (OTI3H1) - Azide and BSA Free [NBP2-73770] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RAB24.](http://images.novusbio.com/fullsize/Rab24-Antibody-OTI3H1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73770-img0003.jpg)
![Western Blot: Rab24 Antibody (OTI3H1) - Azide and BSA Free [NBP2-73770] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Rab24-Antibody-OTI3H1-Azide-and-BSA-Free-Western-Blot-NBP2-73770-img0001.jpg)
![Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-278202415212474.jpg "Western Blot: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Bcl-2 antibody (NBP3-32072) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32072) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-298202414333630.jpg)
![Flow Cytometry: Bcl-2 Antibody (9F3-R) [NBP3-32072] - Flow cytometric analysis of THP-1 cells labeling Bcl-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32072, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32072_mouse-bcl-2-mab-9f3-r-29202416372125.jpg)
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-29820241420941.jpg "Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling PEDFR/PNPLA2/ATGL with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32004, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32004) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-298202414262678.jpg)
![Western Blot: PEDFR/PNPLA2/ATGL Antibody (JE37-49) [NBP3-32004] - Western blot analysis of PEDFR/PNPLA2/ATGL on different lysates with Rabbit anti-PEDFR/PNPLA2/ATGL antibody (NBP3-32004) at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: HEK-293 cell lysate (30 ug/Lane) Lane 4: A431 cell lysate (30 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32004_rabbit-pedfr-pnpla2-atgl-mab-je37-49-392024848502.jpg)
![Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024920238.jpg%3Fv=1666701343 "Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: ATG14 Antibody (1F7C10) [NBP3-27119] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024916246.jpg%3Fv=1666701343)
![Immunohistochemistry: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis of paraffin-embedded testis tissues using ATG14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-104202491703.jpg%3Fv=1666701343)
![Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against human ATG14 (AA: 43-303) recombinant protein. (Expected MW is 56.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024915810.jpg%3Fv=1666701343)
![Western Blot: HeLa Hypoxic Simulated / Normoxic Tissue Lysate [NBP2-36450] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa cobalt chloride treated and normoxic lysates (NBP2-36450). Tubulin loading control is shown below.](http://images.novusbio.com/fullsize/HeLa-Hypoxic-Simulated---Normoxic-Tissue-Lysate-Western-Blot-NBP2-36450-img0002.jpg "Western Blot: HeLa Hypoxic Simulated / Normoxic Tissue Lysate [NBP2-36450] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa cobalt chloride treated and normoxic lysates (NBP2-36450). Tubulin loading control is shown below.")
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-2622024152248.jpg "Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: TOR/mTOR [p Ser2448] Antibody at 0.825ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241140938.jpg)
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Staining of Hela cells with TOR/mTOR [p Ser2448] Antibody (1G6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241312233.jpg)
![Western Blot: Bcl-2 Antibody (BCL2/782) [NBP2-44783] - Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Antibody (BCL2/782).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-782-Western-Blot-NBP2-44783-img0004.jpg "Western Blot: Bcl-2 Antibody (BCL2/782) [NBP2-44783] - Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Antibody (BCL2/782).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/782) [NBP2-44783] - Human Tonsil stained with Bcl-2 Monoclonal Antibody (BCL2/782).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-782-Immunohistochemistry-Paraffin-NBP2-44783-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/782) [NBP2-44783] - Human Melanoma stained with Bcl-2 Monoclonal Antibody (BCL2/782).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-782-Immunohistochemistry-Paraffin-NBP2-44783-img0001.jpg)
![Immunohistochemistry-Paraffin: RGS19 Antibody [NBP3-41987] - DAB staining on IHC-P; Sample: Human Stomach cancer Tissue; Primary Ab: 10ug/ml NBP3-41987; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41987_rabbit-rgs19-pab-612202421331615.jpg "Immunohistochemistry-Paraffin: RGS19 Antibody [NBP3-41987] - DAB staining on IHC-P; Sample: Human Stomach cancer Tissue; Primary Ab: 10ug/ml NBP3-41987; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: RGS19 Antibody [NBP3-41987] - DAB staining on IHC-P; Sample: Human Liver cancer Tissue; Primary Ab: 10ug/ml NBP3-41987; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41987_rabbit-rgs19-pab-612202421373061.jpg)
![Immunohistochemistry-Paraffin: RGS19 Antibody [NBP3-41987] - DAB staining on IHC-P; Sample: Human Lung cancer Tissue; Primary Ab: 10ug/ml NBP3-41987; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41987_rabbit-rgs19-pab-612202421331663.jpg)
![Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-Paraffin-NBP2-19924-img0003.jpg "Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
