| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05380-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92806-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04664-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35185-1mg | |||
| Carrier-Free | NBP2-35185-100ug | |||
| Carrier-Free | NBP2-35185-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98994-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52679-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20094-0.025mg | |||
![Western Blot: PI 3-Kinase p110 delta Antibody (3J0F6) [NBP3-15889] - Western blot analysis of extracts of Mouse thymus, using PI 3-Kinase p110 delta Rabbit mAb (NBP3-15889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-delta-Antibody-3J0F6-Western-Blot-NBP3-15889-img0003.jpg "Western Blot: PI 3-Kinase p110 delta Antibody (3J0F6) [NBP3-15889] - Western blot analysis of extracts of Mouse thymus, using PI 3-Kinase p110 delta Rabbit mAb (NBP3-15889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 delta Antibody (3J0F6) [NBP3-15889] - Immunohistochemistry of paraffin-embedded mouse spleen using PI 3-Kinase p110 delta Rabbit mAb (NBP3-15889) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-delta-Antibody-3J0F6-Immunohistochemistry-Paraffin-NBP3-15889-img0002.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 delta Antibody (3J0F6) [NBP3-15889] - Immunohistochemistry of paraffin-embedded rat spleen using PI 3-Kinase p110 delta Rabbit mAb (NBP3-15889) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PI-3-Kinase-p110-delta-Antibody-3J0F6-Immunohistochemistry-Paraffin-NBP3-15889-img0001.jpg)
![Western Blot: Elk-1 [p Ser383] Antibody [NBP3-05380] - Western blot analysis of extracts of Jurkat cells, using Elk-1 antibody (NBP3-05380) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Elk-1-[p-Ser383]-Antibody-Western-Blot-NBP3-05380-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Elk-1 [p Ser383] Antibody [NBP3-05380] - Western blot analysis of extracts of Jurkat cells, using Elk-1 antibody (NBP3-05380) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-52202516283228.jpg)
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-5220251640288.jpg)
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Mouse cerebellum tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-52202516345237.jpg)
![Immunohistochemistry: Elk-1 [p Ser383] Antibody - BSA Free [Elk-1] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Elk-1 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05380_rabbit-polyclonal-elk-1-p-ser383-antibody-5220251636368.jpg)
![Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of HUH-7 cells, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ATG9B-Antibody-Western-Blot-NBP2-92806-img0001.jpg "Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of HUH-7 cells, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: ATG9B Antibody [NBP2-92806] - Western blot analysis of extracts of various cell lines, using ATG9B antibody (NBP2-92806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ATG9B Antibody-Western Blot-NBP2-92806-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - Azide and BSA Free [NBP2-92806] - Immunofluorescence analysis of human placenta cells using ATG9B Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92806_rabbit-polyclonal-atg9b-antibody-4220251430417.jpg%3Ft=1698886526)
![Western Blot: ATG14 Antibody - Azide and BSA Free [ATG14] - Western blot analysis of various lysates using ATG14 Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-04664_rabbit-polyclonal-atg14-antibody-52202516321486.jpg "Western Blot: ATG14 Antibody - Azide and BSA Free [ATG14] - Western blot analysis of various lysates using ATG14 Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: ATG14 Antibody [NBP3-04664] - Analysis of U2OS cells using ATG14 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-04664_rabbit-polyclonal-atg14-antibody-immunocytochemistry-immunofluorescence-3052024111725..jpg)
![Western Blot: ATG14 Antibody [NBP3-04664] - Analysis of extracts of Neuro-2a cells, using ATG14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/antibody/nbp3-04664_rabbit-polyclonal-atg14-antibody-western-blot-3052024111545..jpg)
![SDS-Page: Mouse TNF-alpha Protein [NBP2-35185]](http://images.novusbio.com/fullsize/Mouse-TNF-alpha-Protein-SDS-Page-NBP2-35185-img0001.jpg "SDS-Page: Mouse TNF-alpha Protein [NBP2-35185]")
![Western Blot: TSG101 Antibody [NBP2-98994] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-98994-img0003.jpg "Western Blot: TSG101 Antibody [NBP2-98994] - Lane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-98994] - Immunofluorescence staining of TSG101 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human TSG101 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98994-img0002.jpg)
![Immunoprecipitation: TSG101 Antibody [NBP2-98994] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-TSG101 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TSG101 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunoprecipitation-NBP2-98994-img0004.jpg)
![Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-29820241435299.jpg "Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32894 at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: K-562 cell lysate (30 µg/Lane) Lane 3: Neuro-2a cell lysate (30 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32894 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32894_-western-blot-639204597482976311.jpg)
![Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924252.png "Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-16D-6B9 (NBP3-20094) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20094, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924253.png)
![Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRAS40-Antibody-Immunohistochemistry-Paraffin-NBP2-19924-img0003.jpg "Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
