| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56576-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20802 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24133 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56002-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21353-25ul | |||
![Immunocytochemistry/Immunofluorescence: ULK1 Antibody [NBP2-56576] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ULK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56576-img0001.jpg "Immunocytochemistry/Immunofluorescence: ULK1 Antibody [NBP2-56576] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: YL1 Antibody [NBP2-56002] - Staining of human cell line MCF7 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/YL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56002-img0001.jpg "Immunocytochemistry/Immunofluorescence: YL1 Antibody [NBP2-56002] - Staining of human cell line MCF7 shows localization to nuclear speckles.")
![Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0002.jpg "Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Untreated (-) and treated (+) Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP1-56995] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP1-56995-img0002.jpg "Western Blot: CHMP4B Antibody [NBP1-56995] - Jurkat cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CHMP4B Antibody [NBP1-56995] - Jurkat Whole Cell lysates, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP1-56995-img0003.jpg)
![Western Blot: Rubicon Antibody [NBP1-30461] - Detection of Human Rubicon by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Rubicon-Antibody-Western-Blot-NBP1-30461-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rubicon Antibody [NBP1-30461] - Detection of Human Rubicon by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Rubicon Antibody [NBP1-30461] - Detection of human Rubicon by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 (lot NBP1-30461-2) used for IP at 6 ug per reaction. Rubicon was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30461-1) and another rabbit anti-Rubicon antibody. For blotting immunoprecipitated Rubicon, NBP1-30461 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Rubicon-Antibody-Immunoprecipitation-NBP1-30461-img0004.jpg)
![Immunohistochemistry;WDFY3 Antibody[NBP3-29117]Detection of human WDFY3 by immunohistochemistry. Sample: FFPE section of human glioblastoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29128_rabbit-wdfy3-pab-7820241037267.jpg "Immunohistochemistry;WDFY3 Antibody[NBP3-29117]Detection of human WDFY3 by immunohistochemistry. Sample: FFPE section of human glioblastoma. Antibody: Affinity purified rabbit anti-WDFY3 used at a dilution of 1:5,000 . Detection: DAB")
![Immunohistochemistry;MDC1 Antibody[NBP3-29608]Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29609_rabbit-mdc1-pab-78202410153551.jpg "Immunohistochemistry;MDC1 Antibody[NBP3-29608]Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 used at a dilution of 1:1,000 . Detection: DAB")
![Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunoprecipitation-NBP3-14688-img0001.jpg "Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16608_rabbit-tsg101-mab-4t4w6-western-blot-22202474218..jpg "<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from wild type (WT) and TSG101/VPS23 knockdown (KD) SH-SY5Y (KD) cells, using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4T4W6) [NBP3-16608] - analysis of lysates from PC-12 cells using TSG101/VPS23 Rabbit mAb at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit. Exposuretime:10s.](http://images.novusbio.com/fullsize/nbp3-16608_rabbit-tsg101-mab-4t4w6-western-blot-22202474338..jpg)