Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit mAb (NBP3-16236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-5N2N9-Western-Blot-NBP3-16236-img0001.jpg "Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit mAb (NBP3-16236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of lysates from wild type (WT) and ATG16L1 knockout (KO) HeLa cells using [KO Validated] ATG16L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16236_rabbit-atg16l1-mab-5n2n9-5220251215428.jpg)
![Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MEK2-Antibody-6D5K1-Western-Blot-NBP3-15651-img0002.jpg "Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody (6D5K1) [NBP3-15651] - Western blot analysis of extracts from normal (control) and MEK2 knockout (KO) HeLa cells, using MEK2 antibody (NBP3-15651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MEK2-Antibody-6D5K1-Knockout-Validated-NBP3-15651-img0001.jpg)
![Western Blot: AKT2 Antibody [NBP3-05341] - Western blot analysis of extracts of various cell lines, using AKT2 antibody (NBP3-05341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/AKT2-Antibody-Western-Blot-NBP3-05341-img0001.jpg "Western Blot: AKT2 Antibody [NBP3-05341] - Western blot analysis of extracts of various cell lines, using AKT2 antibody (NBP3-05341) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: AKT2 Antibody - Azide and BSA Free [NBP3-05341] - Western blot analysis of lysates from 293T cells, using AKT2 Rabbit pAb (A18019) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-05341_mouse-polyclonal-akt2-antibody-52202519302971.jpg)
![Western Blot: VPS11 Antibody (SR1048) [NBP3-22131] - Western blot analysis using NBP3-22131 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22131_rabbit-vps11-mab-sr1048-5720231721518.jpg "Western Blot: VPS11 Antibody (SR1048) [NBP3-22131] - Western blot analysis using NBP3-22131 in K562 cell lysate.")
![Western Blot: PIST Antibody [NB100-1375] - Analysis of PIST/FIG/GOPC on Human Frontal Cortex lysate using this antibody at 0.05 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PIST-Antibody-Western-Blot-NB100-1375-img0001.jpg "Western Blot: PIST Antibody [NB100-1375] - Analysis of PIST/FIG/GOPC on Human Frontal Cortex lysate using this antibody at 0.05 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: VPS29 Antibody [NB100-1387] - (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/VPS29-Antibody-Western-Blot-NB100-1387-img0002.jpg "Western Blot: VPS29 Antibody [NB100-1387] - (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: Jurkat cell lysateLane 3: 293T cell lysateLane 4: MCF7 cell lysateLane 5: K-562 cell lysateLane 6: COS-1 cell lysateLane 7: NIH/3T3 cell lysateLane 8: C2C12 cell lysateLane 9: PC-12 cell lysateLane 10: Mouse brain tissue lysateLane 11: Rat brain tissue lysateLane 12: Mouse kidney tissue lysateLane 13: Mouse colon tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 44 kDaObserved band size: 47 kDaExposure time: 42 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32058_rabbit-atg4b-mab-psh02-53-3920248453542.jpg "Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: Jurkat cell lysateLane 3: 293T cell lysateLane 4: MCF7 cell lysateLane 5: K-562 cell lysateLane 6: COS-1 cell lysateLane 7: NIH/3T3 cell lysateLane 8: C2C12 cell lysateLane 9: PC-12 cell lysateLane 10: Mouse brain tissue lysateLane 11: Rat brain tissue lysateLane 12: Mouse kidney tissue lysateLane 13: Mouse colon tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 44 kDaObserved band size: 47 kDaExposure time: 42 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ATG14 Antibody [NBP2-43577] - Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-43577-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG14 Antibody [NBP2-43577] - Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG14 Antibody [NBP2-43577] - Analysis of A. 20 ug Huh7 whole cell lysate/extract 0 % SDS-PAGE KIAA0831 antibody dilution: 1:1500.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-43577-img0001.jpg)
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249342744.jpg "Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249374967.jpg)