| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70078 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41233-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43982 | |||
| BSA Free | NBP2-61415-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0020.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0016.jpg)
![Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Western-Blot-NBP1-48251-img0018.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0014.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Knockout-Validated-NBP1-48251-img0021.jpg)
![Simple Western:alpha-Actinin 1 Antibody (OTI7A4)[NBP1-48251]Simple Western� analysis of endogenous protein Cytokeratin 8 from HELA lysates (0.5 mg/mL) using Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp1-48251_mouse-alpha-actinin-1-mab-oti7a4-simple-western-13112025123690.jpg%253fstore=america&image-type=image)
![Western Blot: MMP-9 Antibody [NBP2-41233] - Analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/MMP-9-Antibody-Western-Blot-NBP2-41233-img0001.jpg "Western Blot: MMP-9 Antibody [NBP2-41233] - Analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 ug/ml.")
![Immunohistochemistry: MMP-9 Antibody - BSA Free [NBP2-41233] - Immunohistochemistry of MMP-9 in human lung tissue with MMP-9 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41233_rabbit-polyclonal-mmp-9-antibody-271120242045611.jpg)
![Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP2-41233] - Immunofluorescence of MMP-9 in human lung tissue with MMP-9 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41233_rabbit-polyclonal-mmp-9-antibody-2711202420432313.jpg)
![Western Blot: Integrin alpha V/CD51 Antibody (7O8N2) [NBP3-15647] - Analysis of extracts of various cell lines, using Integrin alpha V/CD51 (ITGAV/CD51) antibody (NBP3-15647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-V-CD51-Antibody-7O8N2-Western-Blot-NBP3-15647-img0002.jpg "Western Blot: Integrin alpha V/CD51 Antibody (7O8N2) [NBP3-15647] - Analysis of extracts of various cell lines, using Integrin alpha V/CD51 (ITGAV/CD51) antibody (NBP3-15647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![ICC/IF: Integrin alpha V/CD51 Antibody (7O8N2) [NBP3-15647] -ITGAV Antibody [NBP3-15647] was used (1:100) for labeling and microscopy analysis of the distribution of ITGAV proteins in SUM159 cells. Validated customer review.](http://images.novusbio.com/fullsize/nbp3-15647_rabbit-integrin-alpha-v-cd51-mab-7o8n2-immunocytochemistry-immunofluorescence-1472023105529..jpg)
![Immunohistochemistry: Integrin alpha V/CD51 Antibody (10T9N2) [Integrin alpha V/CD51] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha V/CD51 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15647_rabbit-integrin-alpha-v-cd51-mab-10t9n2-52202517344272.jpg)
![Immunohistochemistry: Integrin alpha V/CD51 Antibody (10T9N2) [Integrin alpha V/CD51] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha V/CD51 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15647_rabbit-integrin-alpha-v-cd51-mab-10t9n2-52202517344277.jpg)
![Immunohistochemistry: Integrin alpha V/CD51 Antibody (10T9N2) [Integrin alpha V/CD51] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Integrin alpha V/CD51 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15647_rabbit-integrin-alpha-v-cd51-mab-10t9n2-5220251734429.jpg)
![Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Western-Blot-NBP3-15638-img0005.jpg "Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of 300ug extracts of 293T cells using 3ug GRB2 antibody (NBP3-15638). Western blot was performed from the immunoprecipitate using GRB2 (NBP3-15638) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunoprecipitation-NBP3-15638-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (9K6H0) [GRB2] - Confocal imaging of HeLa cells using GRB2 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-52202517344210.jpg)
![Immunohistochemistry: GRB2 Antibody (9K6H0) [GRB2] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-52202517333886.jpg)
![Immunohistochemistry: GRB2 Antibody (9K6H0) [GRB2] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-5220251731554.jpg)
![Immunohistochemistry: GRB2 Antibody (9K6H0) [GRB2] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using GRB2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15638_rabbit-grb2-mab-9k6h0-52202517352718.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0012.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0011.jpg)
![Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Flow-Cytometry-NBP2-67327-img0001.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0002.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0003.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0004.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0010.jpg)
![Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Western blot analysis of Neuropilin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Western-Blot-NBP2-67539-img0009.jpg "Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Western blot analysis of Neuropilin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A")
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in MCF-7 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunocytochemistry-Immunofluorescence-NBP2-67539-img0004.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded mouse kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunohistochemistry-Paraffin-NBP2-67539-img0007.jpg)
![Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of Hela cells with Neuropilin-1 antibody (ST05-30) [NBP2-67539] at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Flow-Cytometry-NBP2-67539-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in HUVEC cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunocytochemistry-Immunofluorescence-NBP2-67539-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in SHG-44 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunocytochemistry-Immunofluorescence-NBP2-67539-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunohistochemistry-Paraffin-NBP2-67539-img0005.jpg)
![Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human liver tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-ST05-30-Immunohistochemistry-Paraffin-NBP2-67539-img0006.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0015.jpg "Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Affinity Purified ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ROBO1 Antibody [NB600-1254] - Biotinylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0012.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western blot using Affinity Purified ROBO1 antibody shows detection of a band at ~181 kDa corresponding to ROBO1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0013.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 35 ug per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 181kDa for ROBO1.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0014.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0011.jpg)
![ELISA: ROBO1 Antibody [NB600-1254] - ELISA results of purified ROBO1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/ROBO1-Antibody-ELISA-NB600-1254-img0017.jpg)
![Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MMP-2-Antibody-Western-Blot-NBP2-27208-img0002.jpg "Western Blot: MMP-2 Antibody [NBP2-27208] - Analysis of MMP2 in A: Human MCF7 and B: Mouse RAW lysate using MMP2 antibody at 3 ug/mL.")
![Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Western-Blot-NBP2-61415-img0007.jpg "Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.")
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![ELISA: Human FGF18 ELISA Kit (Colorimetric) [NBP3-06766] - Samples were spiked with high concentrations of Human FGF18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06766_human-fgf18-elisa-kit-colorimetric-13220241626123.jpg "ELISA: Human FGF18 ELISA Kit (Colorimetric) [NBP3-06766] - Samples were spiked with high concentrations of Human FGF18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human FGF18 ELISA Kit (Colorimetric) [NBP3-06766] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06766_human-fgf18-elisa-kit-colorimetric-4320249151632.jpg)
![ELISA: Rat MMP-7 ELISA Kit (Colorimetric) [NBP3-06896] - Samples were spiked with high concentrations of Rat MMP-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06896_rat-mmp-7-elisa-kit-colorimetric-13220241624342.jpg "ELISA: Rat MMP-7 ELISA Kit (Colorimetric) [NBP3-06896] - Samples were spiked with high concentrations of Rat MMP-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat MMP-7 ELISA Kit (Colorimetric) [NBP3-06896] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06896_rat-mmp-7-elisa-kit-colorimetric-43202497363.jpg)
