| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87338-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38026-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-219SS | |||
NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PAWR was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human PAWR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6885-SP | |||
![Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-Western-Blot-NBP1-87338-img0017.jpg "Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in human cell lines Caco-2 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Western-Blot-NBP1-87338-img0010.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0012.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0013.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody [NBP1-87338] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Immunohistochemistry-Paraffin-NBP1-87338-img0014.jpg)
![Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Western blot analysis of extracts of various cell lines, using PAWR / PAR4 Rabbit mAb (NBP3-16523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-10Q2G9-Western-Blot-NBP3-16523-img0003.jpg "Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Western blot analysis of extracts of various cell lines, using PAWR / PAR4 Rabbit mAb (NBP3-16523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunofluorescence analysis of HeLa cells using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-10Q2G9-Immunocytochemistry-Immunofluorescence-NBP3-16523-img0002.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-10Q2G9-Immunohistochemistry-Paraffin-NBP3-16523-img0001.jpg)
![Western Blot: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - PAWR / PAR4 Antibody (3G9H7) Western blot analysis using PAR4 mouse mAb against full-length Trx-Par4 recombinant protein (1) and Hela cell lysate (2).](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-3G9H7-Western-Blot-NBP1-28892-img0006.jpg "Western Blot: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - PAWR / PAR4 Antibody (3G9H7) Western blot analysis using PAR4 mouse mAb against full-length Trx-Par4 recombinant protein (1) and Hela cell lysate (2).")
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - Analysis of human brain (left) and breast carcinoma (right), showing cytoplasmic and membrane localization using PAR4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-3G9H7-Immunohistochemistry-Paraffin-NBP1-28892-img0005.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - Analysis of human Small Intestine tissues using PAR4 mouse mAb.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-3G9H7-Immunohistochemistry-Paraffin-NBP1-28892-img0004.jpg)
![Western Blot: PAWR / PAR4 Antibody [NBP3-13289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PAWR / PAR4 antibody (NBP3-13289) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PAWR---PAR4-Antibody-Western-Blot-NBP3-13289-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PAWR / PAR4 Antibody [NBP3-13289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PAWR / PAR4 antibody (NBP3-13289) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Western Blot: PAWR / PAR4 Antibody [NBP3-13289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAWR / PAR4 antibody (NBP3-13289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13289_rabbit-pawr-par4-pab-910202420122089.jpg)
![Western Blot: PAWR / PAR4 Antibody [NBP3-13289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAWR / PAR4 antibody (NBP3-13289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13289_rabbit-pawr-par4-pab-910202420122046.jpg)
![Western Blot: PAWR / PAR4 Antibody [NBP3-38026] - Western blot analysis of various lysates using PAWR / PAR4 Rabbit pAb at 1:400 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38026_rabbit-pawr-par4-pab-912202414201380.jpg "Western Blot: PAWR / PAR4 Antibody [NBP3-38026] - Western blot analysis of various lysates using PAWR / PAR4 Rabbit pAb at 1:400 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.")
![Western Blot: PAWR / PAR4 Overexpression Lysate (Adult Normal) [NBL1-14127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAWR / PAR4.](http://images.novusbio.com/fullsize/PAWR-PAR4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14127-img0002.jpg "Western Blot: PAWR / PAR4 Overexpression Lysate (Adult Normal) [NBL1-14127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAWR / PAR4.")
