PAWR Antibody

PAWR was detected in immersion fixed LNCaP human prostate cancer cell line using Sheep Anti-Human PAWR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PAWR Antibody Summary

• Immunogen: E. coli-derived recombinant human PAWR
  Arg2-Ala121
  Accession # CAD88640
• Specificity: Detects human PAWR in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PAWR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAWR Antibody

• PAR4
• PAWR
• WT1 Interacting Protein

Background

PAWR (PRKC Apoptosis WT1 Regulator protein; also PAR-4) is an intracellular, 38-42 kDa pro-apoptotic protein. It is widely expressed, and serves multiple functions. WT1 protein is both a transcriptional activator and repressor. When complexed to PAWR, WT1 activation function is repressed, while its repressor activity is enhanced. Thus, PAWR generates transcriptional repression. PAWR also binds to the atypical lambda PKC and zeta PKC isotypes. Such binding inhibits PKC avtivity, blocks cell division and MAPK activation, and promotes Fas-mediated cell apoptosis. Finally, in neurons, PAWR binds to BACE1, promoting the cleavage of APP. Human PAWR is 340 amino acids (aa) in length. It contains an Ala-rich region (aa 49-120), an NLS (aa 145-161), one coiled-coil region (aa 186-206), and a Leu-zipper domain (aa 300-340). There are at least five utilized Ser/Thr phosphorylation sites. PAWR forms noncovalent homodimers and is reported to homooligomerize. There is one potential splice form that shows a three aa substitution for aa 173-340, and a P-P-A-R substitution for A102P103. Full-length PAWR shares 78% aa identity with mouse PAWR.

           

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAWR
• Uniprot:
  • CAD88640()