| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20194-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29858-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25160SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59690-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94478-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47602-0.025mg | |||
![Western Blot: S Opsin Antibody [NBP1-20194] - WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution, incubated overnight at 4C.](http://images.novusbio.com/fullsize/S-Opsin-Antibody-Western-Blot-NBP1-20194-img0003.jpg "Western Blot: S Opsin Antibody [NBP1-20194] - WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution, incubated overnight at 4C.")
![Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/S-Opsin-Antibody-Immunohistochemistry-Paraffin-NBP1-20194-img0002.jpg)
![Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/S-Opsin-Antibody-Immunohistochemistry-Paraffin-NBP1-20194-img0001.jpg)
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2144] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2144-img0005.jpg "Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2144] - Brain, Glioblastoma")
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2144] - Analysis of anti-OPN5 antibody with testis at 30 ug/ml.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2144-img0001.jpg)
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2144] - Analysis of anti-OPN5 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2144-img0002.jpg)
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2144] - Anti-OPN5 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2144-img0003.jpg)
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2144] - Brain, Cortex, neurons and glia](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2144-img0004.jpg)
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2146] - Anti-OPN5 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2146-img0004.jpg "Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2146] - Anti-OPN5 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2146] - Analysis of anti-OPN5 antibody with human ovary, carcinoma.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2146-img0003.jpg)
![Immunohistochemistry-Paraffin: Opsin 5 Antibody [NLS2146] - Anti-OPN5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Immunohistochemistry-Paraffin-NLS2146-img0005.jpg)
![Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2133] - Brain, Medulla](http://images.novusbio.com/fullsize/Opsin-3-Antibody-Immunohistochemistry-Paraffin-NLS2133-img0004.jpg "Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2133] - Brain, Medulla")
![Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2133] - Analysis of anti-Encephalopsin / OPN3 antibody with brain, neurons, and glia](http://images.novusbio.com/fullsize/Opsin-3-Antibody-Immunohistochemistry-Paraffin-NLS2133-img0001.jpg)
![Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2133] - Analysis of anti-Encephalopsin / OPN3 antibody with human lung, small cell carcinoma.](http://images.novusbio.com/fullsize/Opsin-3-Antibody-Immunohistochemistry-Paraffin-NLS2133-img0002.jpg)
![Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2133] - Anti-Encephalopsin / OPN3 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Opsin-3-Antibody-Immunohistochemistry-Paraffin-NLS2133-img0003.jpg)
![Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ABCA4+/+ (left), ABCA4+/- (middle), and ABCA4-/- (right) rod outer segments. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Immunohistochemistry-NBP2-25160-img0004.jpg "Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ABCA4+/+ (left), ABCA4+/- (middle), and ABCA4-/- (right) rod outer segments. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.")
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication. 50 ug of protein samples were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and immunoblotted with the following primary antibodies: ABCA4 (NBP1-30032, 1:1000) and Rhodopsin (NBP2-25160, 1:5000), followed by Anti-Mouse IgG horseradish peroxidase-conjugated secondary antibody (R&D Systems, HAF007, 1:5000. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Western-Blot-NBP2-25160-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Immunocytochemistry-Immunofluorescence-NBP2-25160-img0002.jpg)
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the Rhodopsin protein and appearance of more of this high molecular weight material.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Western-Blot-NBP2-25160-img0003.jpg)
![Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Western-Blot-NBP2-59690-img0005.jpg "Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.")
![Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Immunohistochemistry-NBP2-59690-img0006.jpg)
![Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Western-Blot-NBP2-59690-img0004.jpg)
![Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Immunohistochemistry-Frozen-NBP2-59690-img0002.jpg)
![Western Blot: Opsin 5 Antibody [NBP2-94478] - Analysis of extracts of various cell lines, using Opsin 5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/Opsin-5-Antibody-Western-Blot-NBP2-94478-img0001.jpg "Western Blot: Opsin 5 Antibody [NBP2-94478] - Analysis of extracts of various cell lines, using Opsin 5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Western Blot: Opsin 5 Overexpression Lysate (Adult Normal) [NBP2-04896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Opsin 5.](http://images.novusbio.com/fullsize/Opsin-5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04896-img0001.jpg "Western Blot: Opsin 5 Overexpression Lysate (Adult Normal) [NBP2-04896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Opsin 5.")
![Western Blot: Opsin 3 Overexpression Lysate (Adult Normal) [NBP2-10328] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Opsin 3.](http://images.novusbio.com/fullsize/Opsin-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10328-img0001.jpg "Western Blot: Opsin 3 Overexpression Lysate (Adult Normal) [NBP2-10328] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Opsin 3.")
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Western-Blot-NBP1-47602-img0007.jpg "Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.")
![Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Immunohistochemistry-NBP1-47602-img0006.jpg)
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Western-Blot-NBP1-47602-img0005.jpg)