HAF016). A specific band was detected for TRIM21 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox"> HAF016). A specific band was detected for TRIM21 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human kidney tissue. PVDF Membrane was probed with 0.5 µg/mL of Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for TRIM21 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

285-IF) using Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> 285-IF) using Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

285-IF) using Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> 285-IF) using Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TRIM21 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20 ng/mL of Recombinant Human IFN-gamma (285-IF) using Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF007). A specific band was detected for TRIM21 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox"> HAF007). A specific band was detected for TRIM21 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB62191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for TRIM21 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />