| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0700A488-100 | |||
| Alexa Fluor 546 | DDX0700A546-100 | |||
| Alexa Fluor 647 | DDX0700A647-100 | |||
FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56409SS | |||
MAB002, open histogram) followed by Anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our View our Staining Membrane-associated Proteins protocol." alt="T47D human breast duct carcinoma cell line was stained with Mouse Anti-Human IL-20 Ra Monoclonal Antibody (Catalog # MAB11762, filled histogram) or isotype control antibody (MAB002, open histogram) followed by Anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our View our Staining Membrane-associated Proteins protocol." class="big_thumb" />
CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IL-20 Ra was detected in immersion fixed paraffin-embedded sections of human skin using 8 µg/mL Mouse Anti-Human IL-20 Ra Monoclonal Antibody (Catalog # MAB11761) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11761-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25264 | |||
| Azide and BSA Free | NBP2-25264SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-306-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22376SS | |||
| Azide and BSA Free | NBP2-80812 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1176-SP | |||
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0003.jpg "Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34")
![Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Flow-Cytometry-DDX0700P-100-img0002.jpg)
![Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-R34-34-Immunoprecipitation-DDX0700P-100-img0001.jpg)
![Western Blot: IL-20 R alpha Antibody [NB100-56409] - Analysis of IL-20 R alpha Antibody in A375 cell lysate in the (A) absence and (B) presence of blocking peptide using this antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IL-20-R-alpha-Antibody-Western-Blot-NB100-56409-img0006.jpg "Western Blot: IL-20 R alpha Antibody [NB100-56409] - Analysis of IL-20 R alpha Antibody in A375 cell lysate in the (A) absence and (B) presence of blocking peptide using this antibody at 2 ug/ml.")
![Flow Cytometry: IL-20 R alpha Antibody [NB100-56409] - Cell-surface flow cytometry analysis of IL-20 R alpha Antibodyin A375 cells using this antibody at 5 ug/10^6 cells. The shaded histogram represents cells only, green represents isotype control, and purple represents anti-IL-20 R alpha Antibody](http://images.novusbio.com/fullsize/IL-20-R-alpha-Antibody-Flow-Cytometry-NB100-56409-img0005.jpg)
![Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-73N8F2-Western-Blot-NBP2-25249-img0005.jpg "Western Blot: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Analysis of (A) IL-17R recombinant protein fragment and (B) mouse thymus lysate using IL-7R antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: IL-7 R alpha/CD127 Antibody (73N8F2) [NBP2-25249] - Mouse testis stained with IL-7R antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-7-R-alpha-CD127-Antibody-73N8F2-Immunohistochemistry-Paraffin-NBP2-25249-img0004.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-22F3D9-Western-Blot-NBP2-22376-img0003.jpg "Western Blot: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of CD127 in A) a partial recombinant protein and B) Daudi lysate using CD127 antibody at 0.5 ug/ml and 5 ug/ml respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IL-7R alpha/CD127 Antibody (22F3D9) [NBP2-22376] - Analysis of IL-7 R alpha in human PMBCs using anti-IL-7 R alpha antibody. Image from verified customer review. Image using the Alexa Fluor 405 format of this antibody.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-22F3D9-Flow-Cytometry-NBP2-22376-img0011.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 52 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-278202415242671.jpg "Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 52 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 10% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29820241492652.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunocytochemistry analysis of SiHa cells labeling IL-7R alpha/CD127 with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29202410484826.jpg)
![Flow Cytometry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Flow cytometric analysis of K-562 cells labeling IL-7R alpha/CD127.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32122, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-392024884614.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Western-Blot-NBP3-14749-img0003.jpg "Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0002.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0001.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]- Rabbit anti-CD127/IL-7R alpha recombinantmonoclonal antibody [BLR177J] Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14749_rabbit-il-7r-alpha-cd127-mab-immunohistochemistry-paraffin-1012024122450..jpg)
