![Western Blot: ZNF276 Antibody [NBP1-03342] - Whole cell lysate (50 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF276 antibody usedfor WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/ZNF276-Antibody-Western-Blot-NBP1-03342-img0002.jpg "Western Blot: ZNF276 Antibody [NBP1-03342] - Whole cell lysate (50 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF276 antibody usedfor WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit ZNF276 Antibody - BSA Free (NBP1-03342) is a polyclonal antibody validated for use in WB. Anti-ZNF276 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 564 and 614 of human zinc finger protein 276 using the numbering given in entry NP_001106997.1 (GeneID 92822). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZNF276 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Not recommended for immunoprecipitation. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NBP1-03342 | Applications | Species |
|---|---|---|
| Ito T, Asatsuma-Okumura T, Endo A et al. ZNF276 and WIZ are CRBN neosubstrates involved in the anti-angiogenic activity of thalidomide and immunomodulatory drugs Research Square 2023-11-20 | ||
| Sievers Q L, Petzold G et al. Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN. Science 2018-02-11 [PMID: 30385546] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ZNF276 |
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| Uniprot |
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![Western Blot: ZNF276 Antibody [NBP1-03342] - Whole cell lysate (50 ug) from Jurkat, MCF-7, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF276 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/ZNF276-Antibody-Western-Blot-NBP1-03342-img0001.jpg "Western Blot: ZNF276 Antibody [NBP1-03342] - Whole cell lysate (50 ug) from Jurkat, MCF-7, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZNF276 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")