![Western Blot: V5 Epitope Tag Antibody (SR2319) [NBP3-21864] - Western blot analysis of extracts from V5 Epitope Tag fusion protein, using NBP3-21864.](http://images.novusbio.com/fullsize/nbp3-21864_rabbit-v5-epitope-tag-mab-sr2319-5720231754884.jpg "Western Blot: V5 Epitope Tag Antibody (SR2319) [NBP3-21864] - Western blot analysis of extracts from V5 Epitope Tag fusion protein, using NBP3-21864.")
| Reactivity | ETSpecies Glossary |
| Applications | WB |
| Clone | SR2319 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit V5 Epitope Tag Antibody (SR2319) - BSA Free (NBP3-21864) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthesized peptide |
| Specificity | Detects endogenous levels of V5 Epitope Tag |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, 50% glycerol. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
|
V5 Tags are preferable for detection of Exogenous Proteins in Vivo Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of... Read full blog post. |
|
Working with the V5 Tag Antibody The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")