V2 Vasopressin R/AVPR2 Recombinant Protein Antigen

Product Discontinued

V2 Vasopressin R/AVPR2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human AVPR2.
  
  

  Source: E. coli

  Amino Acid Sequence: SSSVSSELRSLLCCARGRTPPSLGPQDESCTTASSSLAKDTSS

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: AVPR2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14339.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for V2 Vasopressin R/AVPR2 Recombinant Protein Antigen

• ADHR
• ADHRMGC138386
• Antidiuretic hormone receptor
• arginine vasopressin receptor 2
• AVPR V2
• AVPR2
• DI1
• DIR3
• DIRNDI
• NDI
• Renal-type arginine vasopressin receptor
• V2 Vasopressin R
• V2 VasopressinR
• V2R
• V2RMGC126533
• vasopressin V2 receptor

Background

The human AVPR2 locus encodes vasopressin receptor type 2, a member of the vasopressin/oxytocin family subfamily. V2 receptor has been shown to concentrate the urine and maintain water homeostasis by responding to the pituitary hormone arginine vasopressin (AVP). Loss of gene function results in the disease Nephrogenic Diabetes Insipidus (NDI). When extrarenal V2 receptors are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known; its absence does not appear to be detrimental in NDI patients. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts. The V2 Receptor is also expressed outside the kidney although its tissue localization is uncertain. AVPR2 expression has also been described in brain, breast, fetal lung tissue and lung cancer associated with alternative splicing. ESTs have been isolated from normal lung and pancreatic cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AVPR2