V2 Vasopressin R/AVPR2 Antibody

Product Discontinued

V2 Vasopressin R/AVPR2 Antibody Summary

• Immunogen: Peptide with sequence CARGRTPPSLGPQDE corresponding to C-Terminus according to NP_000045.1.
• Epitope: CARGRTPPSLGPQDE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AVPR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 250 kDa band in human brain (cerebellum) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.3 kDa band according to NP_000045.1. The 250 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  V2 Vasopressin R/AVPR2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for V2 Vasopressin R/AVPR2 Antibody

• ADHR
• ADHRMGC138386
• Antidiuretic hormone receptor
• arginine vasopressin receptor 2
• AVPR V2
• AVPR2
• DI1
• DIR3
• DIRNDI
• NDI
• Renal-type arginine vasopressin receptor
• V2 Vasopressin R
• V2 VasopressinR
• V2R
• V2RMGC126533
• vasopressin V2 receptor

Background

The human AVPR2 locus encodes vasopressin receptor type 2, a member of the vasopressin/oxytocin family subfamily. V2 receptor has been shown to concentrate the urine and maintain water homeostasis by responding to the pituitary hormone arginine vasopressin (AVP). Loss of gene function results in the disease Nephrogenic Diabetes Insipidus (NDI). When extrarenal V2 receptors are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known; its absence does not appear to be detrimental in NDI patients. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts. The V2 Receptor is also expressed outside the kidney although its tissue localization is uncertain. AVPR2 expression has also been described in brain, breast, fetal lung tissue and lung cancer associated with alternative splicing. ESTs have been isolated from normal lung and pancreatic cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V2 Vasopressin R/AVPR2 Antibody (NB100-96918)(1)

Publication using NB100-96918

• Mutig K, Paliege A, Kahl T et al. Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL. Am J Physiol Renal Physiol 2007-10-01 [PMID: 17626156]

Bioinformatics

• Gene Symbol: AVPR2
• Entrez:
  • 554(Human)
• Uniprot:
  • NP_000045.1(Human)