Recombinant Human UBE2D4/Ubch5d Protein

Product Discontinued

Recombinant Human UBE2D4/Ubch5d Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-147 of Human UBE2D4/Ubch5d
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2D4
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 10% glycerol, 100 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human UBE2D4/Ubch5d Protein

• EC 6.3.2.19
• FLJ32004
• HBUCE1
• HBUCE1Ubiquitin carrier protein D4
• Ubch5d
• UBE2D4
• ubiquitin-conjugating enzyme E2 D4
• ubiquitin-conjugating enzyme E2D 4 (putative)
• ubiquitin-conjugating enzyme HBUCE1
• Ubiquitin-protein ligase D4

Background

UBE2D4 belongs to the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human UBE2D4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D4