TRMT12 Overexpression Lysate (Native)


Western Blot: TRMT12 Overexpression Lysate (Adult Normal) [NBL1-17323] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRMT12.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

TRMT12 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC203982 and based on accession number NM_017956. The protein contains a C-terminal DDK tag.
Homo sapiens tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for TRMT12

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TRMT12 Overexpression Lysate (Native)

  • Alpha-amino-alpha-carboxypropyl transferase TYW2
  • EC 2.1.1
  • EC
  • EC 2.5.1.-
  • FLJ20772
  • homolog of yeast tRNA methyltransferase
  • TRM12
  • tRNA methyltranferase 12 homolog (S. cerevisiae)
  • tRNA methyltranferase 12 homolog
  • tRNA methyltransferase 12 homolog (S. cerevisiae)
  • tRNA-yW synthesizing protein 2
  • tRNA-yW-synthesizing protein 2
  • TYW2tRNA wybutosine-synthesizing protein 2 homolog


Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol TRMT12