TLR12 Antibody - BSA Free

Western Blot: TLR12 Antibody [NBP2-24833] - Analysis of TLR12 in male rat reproductive tissue: 1) whole testis, 2) caput, 3) corpus, 4) cauda and 5) vas deferens using this antibody at 4 ug/ml.

Flow (Intracellular): TLR12 Antibody [NBP2-24833] - Analysis using the FITC conjugate of NBP2-24833. Staining of TLR12 in the TLR12 table cell line using this antibody at 1 ug/10^6 cells (green represents vector control, red represents TLR12 antibody).

Western Blot: TLR12 Antibody [NBP2-24833] - Analysis of TLR12 in 20 ug/lane of mouse (A) brain, (B) heart, (C) small intestine, (D) kidney tissue lysate using this antibody at 5 ug/ml.

Flow Cytometry: TLR12 Antibody [NBP2-24833] - Analysis using the FITC conjugate of NBP2-24833. Staining of TLR12 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype control was used for this test.

• Reactivity: Mu, Rt
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

TLR12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TLR12 Antibody - BSA Free (NBP2-24833) is a polyclonal antibody validated for use in WB and Flow. Anti-TLR12 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids of 743-756 (RVWLQGLRGKGDKG) of mouse TLR12.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLR12P
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TLR12 Antibody - BSA Free

• Gm1365
• TLR12
• toll-like receptor 12

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLR11 does not respond to known TLR ligands but instead respond specifically to uropathogenic bacteria. TLR11-deficient mice are highly susceptible to infection of the kidneys by uropathogenic bacteria, indicating that TLR11 may play a protective role in preventing infection of internal organs of the urogenital system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR12 Antibody (NBP2-24833)(2)

Publications using NBP2-24833

• Palladino Michael A, Savarese Michael A, Chapman Jessica L et al. Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa. Am J Reprod Immunol. 2008-12-01 [PMID: 19032616]
• Palladino M A, Johnson T A, Gupta R et al. Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract. Biol Reprod. 2007-06-01 [PMID: 17314314] (WB, Rat)

Bioinformatics

• Gene Symbol: TLR12P