TetR Antibody [HRP]

• Reactivity: All-NA
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

TetR Antibody [HRP] Summary

• Immunogen: A synthetic peptide made to an internal portion of Escherichia coli TetR protein (between residues 25-75) [UniProt P04483]
• Localization: TetR peptide conserved in all strains of gram negative bacteria
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for TetR Antibody [HRP]

• pSC101 tetR
• Tet Repressor Protein
• Tet Repressor
• TetR
• tetracycline repressor protein

Background

Tetracycline repressor protein class B from transposon Tn10 (TetR) is a transcription regulator that represses the tetracycline resistance element. The affinity for the tetracycline resistance gene (TetA) promoter operator sites are reduced by the binding of tetracycline to TetR. TetR also binds to DNA on its N-terminal region due to its helix-turn-helix structure (HTH tetR-type DNA-binding domain). The tetracycline resistance element repressed by TetR is responsible for the maintenance of genetic antibiotic resistance traits in strains of Escherichia coli (E.coli). These traits confer an advantage in selection to E. coli expressing this antibiotic resistance (PMID: 18039823).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.