TetR Antibody

Images

 
Western Blot: TetR Antibody [NB600-234] - Analysis of TetR using 23 kDa recombinant protein. Note the presence of a dimer.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.15 mg/ml

TetR Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of Escherichia coli TetR protein (between residues 25-75) [UniProt P04483]
Localization
TetR peptide conserved in all strains of gram negative bacteria
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • ELISA 1:100-1:2000
Application Notes
This TetR antibody is useful for Western Blot and ELISA.
Control Peptide
TetR Peptide (NB600-234PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB600-234 in the following applications:

Publications
Read Publication using NB600-234.

Reactivity Notes

Non-species specific. This antibody is used to monitor the regulation of gene expression in mammalian cells. The TetR antibody is not reactive to human or other mammalian proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TetR Antibody

  • pSC101 tetR
  • Tet Repressor Protein
  • Tet Repressor
  • TetR
  • tetracycline repressor protein

Background

Tetracycline repressor TetR (tetracycline repressor protein class B from transposon Tn10) belongs to the Tet/Cam family of bacterial regulators and it controls the expression of Tn10 encoded tetracycline resistance. When tetracycline is not present, TetR recognises its operator sequence, tetO, for repressing tetA expression (resistance gene) and its own expression. Upon entry into the cell, tetracycline binds a pocket within the protein core of TetR and induces a conformational change leading to reorientation of DNA binding heads, which in turn abolishes operator binding for triggering resistance gene expression. TetR repressed tetracycline resistance element is responsible for the maintenance of antibiotic resistance traits in strains of Escherichia coli which confers an advantage in selection to E. coli expressing tetracycline resistance. Moreover, TetR has attracted widespread interest because regulators belonging to this family control the expression of genes which are involved in development, fatty acid metabolism, drug resistance and many other cellular processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TetR Antibody (NB600-234)(1)

Review for TetR Antibody (NB600-234) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-234:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot TetR NB600-234
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reviewed by:
Ashley Barry
WB Human 12/01/2016
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Summary

ApplicationWestern Blot
Sample Testedcombinant cell lines
SpeciesHuman
LotA-1

Product General Protocols

View specific protocols for TetR Antibody (NB600-234): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TetR Antibody (NB600-234) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-234AF350
Alexa Fluor 405 NB600-234AF405
Alexa Fluor 488 NB600-234AF488
Alexa Fluor 532 NB600-234AF532
Alexa Fluor 594 NB600-234AF594
Alexa Fluor 647 NB600-234AF647
Alexa Fluor 700 NB600-234AF700
Alexa Fluor 750 NB600-234AF750
Biotin NB600-234B
DyLight 350 NB600-234UV
DyLight 405 NB600-234V
DyLight 488 NB600-234G
DyLight 550 NB600-234R
DyLight 594 NB600-234DL594
DyLight 650 NB600-234C
DyLight 680 NB600-234FR
DyLight 755 NB600-234IR
FITC NB600-234F
HRP NB600-234H

Additional TetR Products

Array NB600-234

Research Areas for TetR Antibody (NB600-234)

Find related products by research area.

Blogs on TetR

There are no specific blogs for TetR, but you can read our latest blog posts.
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Contact Information

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Calculators

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Recent Reviews

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1
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1
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Ashley Barry
12/01/2016
Application: WB
Species: Human

Bioinformatics

Entrez
Uniprot