TetR Antibody [DyLight 550]

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Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

TetR Antibody [DyLight 550] Summary

Immunogen
A synthetic peptide made to an internal portion of Escherichia coli TetR protein (between residues 25-75) [UniProt P04483]
Localization
TetR peptide conserved in all strains of gram negative bacteria
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
This TetR antibody is useful for Western Blot and ELISA.

Reactivity Notes

Non-species specific. This antibody is used to monitor the regulation of gene expression in mammalian cells. The TetR antibody is not reactive to human or other mammalian proteins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TetR Antibody [DyLight 550]

  • pSC101 tetR
  • Tet Repressor Protein
  • Tet Repressor
  • TetR
  • tetracycline repressor protein

Background

Tetracycline repressor protein class B from transposon Tn10 (TetR) is a transcription regulator that represses the tetracycline resistance element. The affinity for the tetracycline resistance gene (TetA) promoter operator sites are reduced by the binding of tetracycline to TetR. TetR also binds to DNA on its N-terminal region due to its helix-turn-helix structure (HTH tetR-type DNA-binding domain). The tetracycline resistance element repressed by TetR is responsible for the maintenance of genetic antibiotic resistance traits in strains of Escherichia coli (E.coli). These traits confer an advantage in selection to E. coli expressing this antibiotic resistance (PMID: 18039823).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TetR Antibody (NB600-234R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-234AF350
Alexa Fluor 405 NB600-234AF405
Alexa Fluor 488 NB600-234AF488
Alexa Fluor 532 NB600-234AF532
Alexa Fluor 594 NB600-234AF594
Alexa Fluor 647 NB600-234AF647
Alexa Fluor 700 NB600-234AF700
Alexa Fluor 750 NB600-234AF750
Biotin NB600-234B
DyLight 350 NB600-234UV
DyLight 405 NB600-234V
DyLight 488 NB600-234G
DyLight 550 NB600-234R
DyLight 594 NB600-234DL594
DyLight 650 NB600-234C
DyLight 680 NB600-234FR
DyLight 755 NB600-234IR
FITC NB600-234F
HRP NB600-234H

Additional TetR Products

Array NB600-234R

Research Areas for TetR Antibody (NB600-234R)

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Blogs on TetR

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Calculators

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